Aaron Lun
| Snapshot Date: 2020-10-16 14:40:19 -0400 (Fri, 16 Oct 2020) |
| URL: https://git.bioconductor.org/packages/diffHic |
| Branch: RELEASE_3_11 |
| Last Commit: ca6d240 |
| Last Changed Date: 2020-04-27 14:48:43 -0400 (Mon, 27 Apr 2020) |
| Back to Multiple platform build/check report for BioC 3.11 |
|
This page was generated on 2020-10-17 11:54:40 -0400 (Sat, 17 Oct 2020).
| TO THE DEVELOPERS/MAINTAINERS OF THE diffHic PACKAGE: Please make sure to use the following settings in order to reproduce any error or warning you see on this page. |
| Package 473/1905 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||
| diffHic 1.20.0 Aaron Lun
| malbec2 | Linux (Ubuntu 18.04.4 LTS) / x86_64 | OK | OK | [ OK ] | | ||||||
| tokay2 | Windows Server 2012 R2 Standard / x64 | OK | OK | WARNINGS | OK | | ||||||
| machv2 | macOS 10.14.6 Mojave / x86_64 | OK | OK | OK | OK | |
| Package: diffHic |
| Version: 1.20.0 |
| Command: /home/biocbuild/bbs-3.11-bioc/R/bin/R CMD check --install=check:diffHic.install-out.txt --library=/home/biocbuild/bbs-3.11-bioc/R/library --no-vignettes --timings diffHic_1.20.0.tar.gz |
| StartedAt: 2020-10-17 00:44:35 -0400 (Sat, 17 Oct 2020) |
| EndedAt: 2020-10-17 00:52:38 -0400 (Sat, 17 Oct 2020) |
| EllapsedTime: 483.1 seconds |
| RetCode: 0 |
| Status: OK |
| CheckDir: diffHic.Rcheck |
| Warnings: 0 |
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###
### Running command:
###
### /home/biocbuild/bbs-3.11-bioc/R/bin/R CMD check --install=check:diffHic.install-out.txt --library=/home/biocbuild/bbs-3.11-bioc/R/library --no-vignettes --timings diffHic_1.20.0.tar.gz
###
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* using log directory ‘/home/biocbuild/bbs-3.11-bioc/meat/diffHic.Rcheck’
* using R version 4.0.3 (2020-10-10)
* using platform: x86_64-pc-linux-gnu (64-bit)
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘diffHic/DESCRIPTION’ ... OK
* this is package ‘diffHic’ version ‘1.20.0’
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘diffHic’ can be installed ... OK
* checking installed package size ... NOTE
installed size is 11.6Mb
sub-directories of 1Mb or more:
doc 1.3Mb
libs 9.0Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking line endings in Makefiles ... OK
* checking compilation flags in Makevars ... OK
* checking for GNU extensions in Makefiles ... NOTE
GNU make is a SystemRequirements.
* checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK
* checking use of PKG_*FLAGS in Makefiles ... OK
* checking compiled code ... NOTE
Note: information on .o files is not available
File ‘/home/biocbuild/bbs-3.11-bioc/R/library/diffHic/libs/diffHic.so’:
Found ‘__printf_chk’, possibly from ‘printf’ (C)
Found ‘abort’, possibly from ‘abort’ (C)
Found ‘exit’, possibly from ‘exit’ (C)
Found ‘putchar’, possibly from ‘putchar’ (C)
Found ‘puts’, possibly from ‘printf’ (C), ‘puts’ (C)
Found ‘srand48’, possibly from ‘srand48’ (C)
Found ‘stderr’, possibly from ‘stderr’ (C)
Found ‘stdout’, possibly from ‘stdout’ (C)
Compiled code should not call entry points which might terminate R nor
write to stdout/stderr instead of to the console, nor use Fortran I/O
nor system RNGs. The detected symbols are linked into the code but
might come from libraries and not actually be called.
See ‘Writing portable packages’ in the ‘Writing R Extensions’ manual.
* checking installed files from ‘inst/doc’ ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
normalizeCNV 12.979 0.012 13.042
cutGenome 8.635 0.107 9.345
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
Running ‘test-basic.R’
Comparing ‘test-basic.Rout’ to ‘test-basic.Rout.save’ ...208,210d207
< Warning message:
< In (function (counts = matrix(0, 0, 0), lib.size = colSums(counts), :
< norm factors don't multiply to 1
Running ‘testthat.R’
OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE
Status: 3 NOTEs
See
‘/home/biocbuild/bbs-3.11-bioc/meat/diffHic.Rcheck/00check.log’
for details.
diffHic.Rcheck/00install.out
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###
### Running command:
###
### /home/biocbuild/bbs-3.11-bioc/R/bin/R CMD INSTALL diffHic
###
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* installing to library ‘/home/biocbuild/bbs-3.11-bioc/R/library’
* installing *source* package ‘diffHic’ ...
** using staged installation
** libs
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c binner.cpp -o binner.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c check_input.cpp -o check_input.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c cluster_2d.cpp -o cluster_2d.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c count_background.cpp -o count_background.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c count_connect.cpp -o count_connect.o
count_connect.cpp: In function ‘SEXPREC* count_connect(SEXP, SEXP)’:
count_connect.cpp:57:30: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]
for (size_t odex=0; odex < ncombos; ++odex) {
~~~~~^~~~~~~~~
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c count_patch.cpp -o count_patch.o
count_patch.cpp: In function ‘SEXPREC* count_patch(SEXP, SEXP, SEXP, SEXP, SEXP)’:
count_patch.cpp:45:33: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]
for (size_t vecdex=0; vecdex<ncombos; ++vecdex) {
~~~~~~^~~~~~~~
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c directionality.cpp -o directionality.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c init.cpp -o init.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c iterative_correction.cpp -o iterative_correction.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c neighbors.cpp -o neighbors.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c pair_stats.cpp -o pair_stats.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c quadrant_bg.cpp -o quadrant_bg.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c report_hic_pairs.cpp -o report_hic_pairs.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c trended_filter.cpp -o trended_filter.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.11-bioc/R/include" -DNDEBUG -D_FILE_OFFSET_BITS=64 -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/zlibbioc/include' -I'/home/biocbuild/bbs-3.11-bioc/R/library/Rcpp/include' -I/usr/local/include -fpic -g -O2 -Wall -c utils.cpp -o utils.o
g++ -std=gnu++11 -shared -L/home/biocbuild/bbs-3.11-bioc/R/lib -L/usr/local/lib -o diffHic.so binner.o check_input.o cluster_2d.o count_background.o count_connect.o count_patch.o directionality.o init.o iterative_correction.o neighbors.o pair_stats.o quadrant_bg.o report_hic_pairs.o trended_filter.o utils.o /home/biocbuild/bbs-3.11-bioc/R/library/Rhtslib/usrlib/libhts.a -lcurl -L/home/biocbuild/bbs-3.11-bioc/R/lib -lR
installing to /home/biocbuild/bbs-3.11-bioc/R/library/00LOCK-diffHic/00new/diffHic/libs
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
‘diffHic.Rnw’
** testing if installed package can be loaded from temporary location
** checking absolute paths in shared objects and dynamic libraries
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (diffHic)
diffHic.Rcheck/tests/test-basic.Rout
R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> # These are just placeholders for the real things in inst/tests.
>
> suppressWarnings(suppressPackageStartupMessages(require(diffHic)))
>
> hic.file <- system.file("exdata", "hic_sort.bam", package="diffHic")
> cuts <- readRDS(system.file("exdata", "cuts.rds", package="diffHic"))
> param <- pairParam(fragments=cuts)
>
> # Setting up the parameters
> fout <- "output.h5"
> preparePairs(hic.file, param, file=fout)
$pairs
total marked filtered mapped
32 7 3 22
$same.id
dangling self.circle
4 1
$singles
[1] 2
$chimeras
total mapped multi invalid
12 8 7 5
> head(getPairData(fout, param))
length orientation insert
1 40 1 40
2 80 1 80
3 60 2 54
4 40 3 160
5 20 1 66
6 80 0 114
>
> loadChromos(fout)
anchor1 anchor2
1 chrA chrA
2 chrB chrA
3 chrB chrB
> head(loadData(fout, "chrA", "chrA"))
anchor1.id anchor2.id anchor1.pos anchor2.pos anchor1.len anchor2.len
1 2 1 49 14 -5 10
2 2 1 79 9 -10 10
3 2 1 65 21 10 -10
4 4 1 156 6 -10 -10
5 4 2 141 80 -5 10
6 4 2 154 50 10 10
> head(loadData(fout, "chrA", "chrB"))
anchor1.id anchor2.id anchor1.pos anchor2.pos anchor1.len anchor2.len
1 5 2 19 44 10 -10
2 5 3 14 105 10 -10
3 6 1 65 19 5 10
4 6 2 24 90 -5 5
5 6 3 24 100 -5 -10
6 6 3 24 95 -5 -10
Warning message:
In value[[3L]](cond) : anchor definitions are reversed
>
> # Loading the counts.
> data <- squareCounts(fout, param, width=50, filter=1)
> data
class: InteractionSet
dim: 10 1
metadata(2): param width
assays(1): counts
rownames: NULL
rowData names(0):
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 6
>
> margins <- marginCounts(fout, param, width=50)
> margins
class: RangedSummarizedExperiment
dim: 6 1
metadata(1): param
assays(1): counts
rownames: NULL
rowData names(1): nfrags
colnames: NULL
colData names(1): totals
> totalCounts(fout, param)
[1] 17
>
> regions <- GRanges("chrA", IRanges(c(1, 100, 150), c(20, 140, 160)))
> connectCounts(fout, param, regions=regions, filter=1L)
class: InteractionSet
dim: 2 1
metadata(1): param
assays(1): counts
rownames: NULL
rowData names(0):
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 3
>
> # Checking some values.
> head(getArea(data))
[1] 2208 2304 2208 2208 3312 3174
> head(pairdist(data))
[1] 47 140 93 47 NA NA
>
> anchors(data, type="first")
GRanges object with 10 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 49-94 * | 1
[2] chrA 141-188 * | 1
[3] chrA 141-188 * | 1
[4] chrA 141-188 * | 1
[5] chrB 1-69 * | 2
[6] chrB 1-69 * | 2
[7] chrB 1-69 * | 2
[8] chrB 1-69 * | 2
[9] chrB 70-92 * | 1
[10] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
> anchors(data, type="second")
GRanges object with 10 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 1-48 * | 1
[2] chrA 1-48 * | 1
[3] chrA 49-94 * | 1
[4] chrA 95-140 * | 1
[5] chrA 1-48 * | 1
[6] chrA 49-94 * | 1
[7] chrA 95-140 * | 1
[8] chrB 1-69 * | 2
[9] chrB 1-69 * | 2
[10] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
> assay(data)
[,1]
[1,] 3
[2,] 1
[3,] 2
[4,] 1
[5,] 1
[6,] 2
[7,] 3
[8,] 1
[9,] 2
[10,] 1
> regions(data)
GRanges object with 6 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 1-48 * | 1
[2] chrA 49-94 * | 1
[3] chrA 95-140 * | 1
[4] chrA 141-188 * | 1
[5] chrB 1-69 * | 2
[6] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
>
> data$totals
[1] 17
> colData(data)
DataFrame with 1 row and 1 column
totals
<integer>
1 17
> metadata(data)
$param
Genome contains 7 restriction fragments across 2 chromosomes
No discard regions are specified
No limits on chromosomes for read extraction
No cap on the read pairs per pair of restriction fragments
$width
[1] 50
>
> asDGEList(data)
An object of class "DGEList"
$counts
Sample1
1 3
2 1
3 2
4 1
5 1
6 2
7 3
8 1
9 2
10 1
$samples
group lib.size norm.factors
Sample1 1 17 1
> asDGEList(data, lib.size=20)$samples
group lib.size norm.factors
Sample1 1 20 1
> asDGEList(data, norm.factors=2, group="a")$samples
group lib.size norm.factors
Sample1 a 17 2
Warning message:
In (function (counts = matrix(0, 0, 0), lib.size = colSums(counts), :
norm factors don't multiply to 1
>
> # Playing around with some bin counts.
> stuff <- correctedContact(data)
> head(stuff$truth)
[1] 1.517032e+04 6.123523e-01 6.123523e-01 8.162749e-01 2.041633e-01
[6] 2.041633e-01
>
> data.large <- squareCounts(fout, param, width=100, filter=1)
> boxed <- boxPairs(larger=data.large, smaller=data)
> head(boxed$indices$larger)
[1] 1 2 3 4 5 6
> head(boxed$indices$smaller)
[1] 1 2 2 3 4 4
>
> head(enrichedPairs(data))
class: InteractionSet
dim: 6 1
metadata(2): param width
assays(5): counts quadrant vertical horizontal surrounding
rownames: NULL
rowData names(4): N.quadrant N.vertical N.horizontal N.surrounding
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 6
> head(clusterPairs(data, tol=10)$indices[[1]])
[1] 1 2 2 2 3 3
>
> # End.
>
> unlink(fout)
>
> proc.time()
user system elapsed
17.426 0.540 20.672
diffHic.Rcheck/tests/test-basic.Rout.save
R version 3.5.0 Patched (2018-04-30 r74679) -- "Joy in Playing"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
Natural language support but running in an English locale
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> # These are just placeholders for the real things in inst/tests.
>
> suppressWarnings(suppressPackageStartupMessages(require(diffHic)))
>
> hic.file <- system.file("exdata", "hic_sort.bam", package="diffHic")
> cuts <- readRDS(system.file("exdata", "cuts.rds", package="diffHic"))
> param <- pairParam(fragments=cuts)
>
> # Setting up the parameters
> fout <- "output.h5"
> preparePairs(hic.file, param, file=fout)
$pairs
total marked filtered mapped
32 7 3 22
$same.id
dangling self.circle
4 1
$singles
[1] 2
$chimeras
total mapped multi invalid
12 8 7 5
> head(getPairData(fout, param))
length orientation insert
1 40 1 40
2 80 1 80
3 60 2 54
4 40 3 160
5 20 1 66
6 80 0 114
>
> loadChromos(fout)
anchor1 anchor2
1 chrA chrA
2 chrB chrA
3 chrB chrB
> head(loadData(fout, "chrA", "chrA"))
anchor1.id anchor2.id anchor1.pos anchor2.pos anchor1.len anchor2.len
1 2 1 49 14 -5 10
2 2 1 79 9 -10 10
3 2 1 65 21 10 -10
4 4 1 156 6 -10 -10
5 4 2 141 80 -5 10
6 4 2 154 50 10 10
> head(loadData(fout, "chrA", "chrB"))
anchor1.id anchor2.id anchor1.pos anchor2.pos anchor1.len anchor2.len
1 5 2 19 44 10 -10
2 5 3 14 105 10 -10
3 6 1 65 19 5 10
4 6 2 24 90 -5 5
5 6 3 24 100 -5 -10
6 6 3 24 95 -5 -10
Warning message:
In value[[3L]](cond) : anchor definitions are reversed
>
> # Loading the counts.
> data <- squareCounts(fout, param, width=50, filter=1)
> data
class: InteractionSet
dim: 10 1
metadata(2): param width
assays(1): counts
rownames: NULL
rowData names(0):
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 6
>
> margins <- marginCounts(fout, param, width=50)
> margins
class: RangedSummarizedExperiment
dim: 6 1
metadata(1): param
assays(1): counts
rownames: NULL
rowData names(1): nfrags
colnames: NULL
colData names(1): totals
> totalCounts(fout, param)
[1] 17
>
> regions <- GRanges("chrA", IRanges(c(1, 100, 150), c(20, 140, 160)))
> connectCounts(fout, param, regions=regions, filter=1L)
class: InteractionSet
dim: 2 1
metadata(1): param
assays(1): counts
rownames: NULL
rowData names(0):
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 3
>
> # Checking some values.
> head(getArea(data))
[1] 2208 2304 2208 2208 3312 3174
> head(pairdist(data))
[1] 47 140 93 47 NA NA
>
> anchors(data, type="first")
GRanges object with 10 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 49-94 * | 1
[2] chrA 141-188 * | 1
[3] chrA 141-188 * | 1
[4] chrA 141-188 * | 1
[5] chrB 1-69 * | 2
[6] chrB 1-69 * | 2
[7] chrB 1-69 * | 2
[8] chrB 1-69 * | 2
[9] chrB 70-92 * | 1
[10] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
> anchors(data, type="second")
GRanges object with 10 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 1-48 * | 1
[2] chrA 1-48 * | 1
[3] chrA 49-94 * | 1
[4] chrA 95-140 * | 1
[5] chrA 1-48 * | 1
[6] chrA 49-94 * | 1
[7] chrA 95-140 * | 1
[8] chrB 1-69 * | 2
[9] chrB 1-69 * | 2
[10] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
> assay(data)
[,1]
[1,] 3
[2,] 1
[3,] 2
[4,] 1
[5,] 1
[6,] 2
[7,] 3
[8,] 1
[9,] 2
[10,] 1
> regions(data)
GRanges object with 6 ranges and 1 metadata column:
seqnames ranges strand | nfrags
<Rle> <IRanges> <Rle> | <integer>
[1] chrA 1-48 * | 1
[2] chrA 49-94 * | 1
[3] chrA 95-140 * | 1
[4] chrA 141-188 * | 1
[5] chrB 1-69 * | 2
[6] chrB 70-92 * | 1
-------
seqinfo: 2 sequences from an unspecified genome
>
> data$totals
[1] 17
> colData(data)
DataFrame with 1 row and 1 column
totals
<integer>
1 17
> metadata(data)
$param
Genome contains 7 restriction fragments across 2 chromosomes
No discard regions are specified
No limits on chromosomes for read extraction
No cap on the read pairs per pair of restriction fragments
$width
[1] 50
>
> asDGEList(data)
An object of class "DGEList"
$counts
Sample1
1 3
2 1
3 2
4 1
5 1
6 2
7 3
8 1
9 2
10 1
$samples
group lib.size norm.factors
Sample1 1 17 1
> asDGEList(data, lib.size=20)$samples
group lib.size norm.factors
Sample1 1 20 1
> asDGEList(data, norm.factors=2, group="a")$samples
group lib.size norm.factors
Sample1 a 17 2
>
> # Playing around with some bin counts.
> stuff <- correctedContact(data)
> head(stuff$truth)
[1] 1.517032e+04 6.123523e-01 6.123523e-01 8.162749e-01 2.041633e-01
[6] 2.041633e-01
>
> data.large <- squareCounts(fout, param, width=100, filter=1)
> boxed <- boxPairs(larger=data.large, smaller=data)
> head(boxed$indices$larger)
[1] 1 2 3 4 5 6
> head(boxed$indices$smaller)
[1] 1 2 2 3 4 4
>
> head(enrichedPairs(data))
class: InteractionSet
dim: 6 1
metadata(2): param width
assays(5): counts quadrant vertical horizontal surrounding
rownames: NULL
rowData names(4): N.quadrant N.vertical N.horizontal N.surrounding
colnames: NULL
colData names(1): totals
type: ReverseStrictGInteractions
regions: 6
> head(clusterPairs(data, tol=10)$indices[[1]])
[1] 1 2 2 2 3 3
>
> # End.
>
> unlink(fout)
>
> proc.time()
user system elapsed
5.571 0.168 5.757
diffHic.Rcheck/tests/testthat.Rout
R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(diffHic)
Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colnames, dirname, do.call,
duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
tapply, union, unique, unsplit, which, which.max, which.min
Loading required package: S4Vectors
Attaching package: 'S4Vectors'
The following object is masked from 'package:base':
expand.grid
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: InteractionSet
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: DelayedArray
Loading required package: matrixStats
Attaching package: 'matrixStats'
The following objects are masked from 'package:Biobase':
anyMissing, rowMedians
Attaching package: 'DelayedArray'
The following objects are masked from 'package:matrixStats':
colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges
The following objects are masked from 'package:base':
aperm, apply, rowsum
>
> test_check("diffHic")
══ testthat results ═══════════════════════════════════════════════════════════
[ OK: 427 | SKIPPED: 0 | WARNINGS: 0 | FAILED: 0 ]
>
> proc.time()
user system elapsed
67.181 0.894 71.108
diffHic.Rcheck/diffHic-Ex.timings
| name | user | system | elapsed | |
| DNaseHiC | 0.258 | 0.000 | 0.258 | |
| annotatePairs | 1.223 | 0.000 | 1.224 | |
| boxPairs | 1.021 | 0.048 | 1.069 | |
| clusterPairs | 0.915 | 0.000 | 0.950 | |
| compartmentalize | 0.684 | 0.004 | 0.688 | |
| connectCounts | 1.789 | 0.013 | 1.825 | |
| consolidatePairs | 0.628 | 0.000 | 0.628 | |
| correctedContact | 0.409 | 0.000 | 0.412 | |
| cutGenome | 8.635 | 0.107 | 9.345 | |
| diClusters | 2.800 | 0.000 | 3.061 | |
| diffHicUsersGuide | 0.001 | 0.000 | 0.001 | |
| domainDirections | 0.204 | 0.000 | 0.204 | |
| enrichedPairs | 0.735 | 0.000 | 0.837 | |
| extractPatch | 0.813 | 0.004 | 0.818 | |
| filterDiag | 0.191 | 0.004 | 0.229 | |
| filterPeaks | 0.369 | 0.000 | 0.373 | |
| filters | 0.371 | 0.004 | 0.378 | |
| getArea | 0.163 | 0.000 | 0.201 | |
| getPairData | 0.051 | 0.000 | 0.051 | |
| loadData | 0.066 | 0.000 | 0.066 | |
| marginCounts | 0.785 | 0.000 | 0.842 | |
| mergeCMs | 0.830 | 0.000 | 1.133 | |
| mergePairs | 0.164 | 0.008 | 0.172 | |
| neighborCounts | 0.168 | 0.000 | 0.167 | |
| normalizeCNV | 12.979 | 0.012 | 13.042 | |
| pairParam | 0.16 | 0.00 | 0.16 | |
| plotDI | 0.754 | 0.000 | 0.753 | |
| plotPlaid | 2.315 | 0.008 | 2.323 | |
| preparePairs | 0.231 | 0.000 | 0.231 | |
| prunePairs | 0.275 | 0.008 | 0.283 | |
| readMTX2IntSet | 0.322 | 0.000 | 0.321 | |
| savePairs | 0.049 | 0.000 | 0.049 | |
| squareCounts | 0.719 | 0.004 | 0.724 | |
| totalCounts | 0.424 | 0.004 | 0.428 | |