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CHECK report for PureCN on tokay1

This page was generated on 2020-04-15 12:26:37 -0400 (Wed, 15 Apr 2020).

Package 1330/1823HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
PureCN 1.16.0
Markus Riester
Snapshot Date: 2020-04-14 16:46:13 -0400 (Tue, 14 Apr 2020)
URL: https://git.bioconductor.org/packages/PureCN
Branch: RELEASE_3_10
Last Commit: d5c94ec
Last Changed Date: 2019-10-29 17:25:55 -0400 (Tue, 29 Oct 2019)
malbec1 Linux (Ubuntu 18.04.4 LTS) / x86_64  OK  OK  OK UNNEEDED, same version exists in internal repository
tokay1 Windows Server 2012 R2 Standard / x64  OK  OK [ OK ] OK UNNEEDED, same version exists in internal repository
merida1 OS X 10.11.6 El Capitan / x86_64  OK  OK  OK  OK UNNEEDED, same version exists in internal repository

Summary

Package: PureCN
Version: 1.16.0
Command: C:\Users\biocbuild\bbs-3.10-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:PureCN.install-out.txt --library=C:\Users\biocbuild\bbs-3.10-bioc\R\library --no-vignettes --timings PureCN_1.16.0.tar.gz
StartedAt: 2020-04-15 05:44:21 -0400 (Wed, 15 Apr 2020)
EndedAt: 2020-04-15 06:17:26 -0400 (Wed, 15 Apr 2020)
EllapsedTime: 1985.1 seconds
RetCode: 0
Status:  OK  
CheckDir: PureCN.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   C:\Users\biocbuild\bbs-3.10-bioc\R\bin\R.exe CMD check --force-multiarch --install=check:PureCN.install-out.txt --library=C:\Users\biocbuild\bbs-3.10-bioc\R\library --no-vignettes --timings PureCN_1.16.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory 'C:/Users/biocbuild/bbs-3.10-bioc/meat/PureCN.Rcheck'
* using R version 3.6.3 (2020-02-29)
* using platform: x86_64-w64-mingw32 (64-bit)
* using session charset: ISO8859-1
* using option '--no-vignettes'
* checking for file 'PureCN/DESCRIPTION' ... OK
* checking extension type ... Package
* this is package 'PureCN' version '1.16.0'
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking whether package 'PureCN' can be installed ... OK
* checking installed package size ... NOTE
  installed size is  5.8Mb
  sub-directories of 1Mb or more:
    data      1.1Mb
    extdata   3.8Mb
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* loading checks for arch 'i386'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* loading checks for arch 'x64'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of 'data' directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking files in 'vignettes' ... OK
* checking examples ...
** running examples for arch 'i386' ... OK
Examples with CPU or elapsed time > 5s
                               user system elapsed
callAmplificationsInLowPurity 46.54   0.07   46.63
segmentationPSCBS             28.81   0.24   29.14
filterIntervals               22.16   0.06   22.27
runAbsoluteCN                 16.87   0.05   16.89
segmentationHclust            15.67   0.09   15.72
findFocal                      8.66   0.05    8.86
segmentationCBS                8.52   0.00    8.51
annotateTargets                7.95   0.30    9.42
processMultipleSamples         6.71   0.08    7.37
** running examples for arch 'x64' ... OK
Examples with CPU or elapsed time > 5s
                               user system elapsed
callAmplificationsInLowPurity 45.57   0.06   45.65
filterIntervals               29.56   0.05   29.65
segmentationPSCBS             28.48   0.34   28.91
runAbsoluteCN                 19.75   0.03   19.78
segmentationHclust            13.08   0.03   13.11
segmentationCBS                9.33   0.02    9.35
findFocal                      8.75   0.00    8.75
processMultipleSamples         6.97   0.00    6.96
annotateTargets                5.56   0.23    5.80
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
** running tests for arch 'i386' ...
  Running 'testthat.R'
 OK
** running tests for arch 'x64' ...
  Running 'testthat.R'
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in 'inst/doc' ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 1 NOTE
See
  'C:/Users/biocbuild/bbs-3.10-bioc/meat/PureCN.Rcheck/00check.log'
for details.

Installation output

PureCN.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   C:\cygwin\bin\curl.exe -O https://malbec1.bioconductor.org/BBS/3.10/bioc/src/contrib/PureCN_1.16.0.tar.gz && rm -rf PureCN.buildbin-libdir && mkdir PureCN.buildbin-libdir && C:\Users\biocbuild\bbs-3.10-bioc\R\bin\R.exe CMD INSTALL --merge-multiarch --build --library=PureCN.buildbin-libdir PureCN_1.16.0.tar.gz && C:\Users\biocbuild\bbs-3.10-bioc\R\bin\R.exe CMD INSTALL PureCN_1.16.0.zip && rm PureCN_1.16.0.tar.gz PureCN_1.16.0.zip
###
##############################################################################
##############################################################################


  % Total    % Received % Xferd  Average Speed   Time    Time     Time  Current
                                 Dload  Upload   Total   Spent    Left  Speed

  0     0    0     0    0     0      0      0 --:--:-- --:--:-- --:--:--     0
100 2957k  100 2957k    0     0  8343k      0 --:--:-- --:--:-- --:--:-- 8424k

install for i386

* installing *source* package 'PureCN' ...
** using staged installation
** R
** data
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
  converting help for package 'PureCN'
    finding HTML links ... done
    PureCN-defunct                          html  
    PureCN-deprecated                       html  
    annotateTargets                         html  
    bootstrapResults                        html  
    calculateBamCoverageByInterval          html  
    calculateGCContentByInterval            html  
    calculateIntervalWeights                html  
    calculateLogRatio                       html  
    calculateMappingBiasVcf                 html  
    calculatePowerDetectSomatic             html  
    calculateTangentNormal                  html  
    callAlterations                         html  
    callAlterationsFromSegmentation         html  
    callAmplificationsInLowPurity           html  
    callCIN                                 html  
    callLOH                                 html  
    callMutationBurden                      html  
    centromeres                             html  
    correctCoverageBias                     html  
    createCurationFile                      html  
    createNormalDatabase                    html  
    createTargetWeights                     html  
    filterIntervals                         html  
    filterTargets                           html  
    filterVcfBasic                          html  
    filterVcfMuTect                         html  
    filterVcfMuTect2                        html  
    findFocal                               html  
    getSexFromCoverage                      html  
    getSexFromVcf                           html  
    plotAbs                                 html  
    poolCoverage                            html  
    predictSomatic                          html  
    preprocessIntervals                     html  
    processMultipleSamples                  html  
    purecn.DNAcopy.bdry                     html  
    purecn.example.output                   html  
    readCoverageFile                        html  
    readCurationFile                        html  
    readLogRatioFile                        html  
    readSegmentationFile                    html  
    runAbsoluteCN                           html  
    segmentationCBS                         html  
    segmentationHclust                      html  
    segmentationPSCBS                       html  
    setMappingBiasVcf                       html  
    setPriorVcf                             html  
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path

install for x64

* installing *source* package 'PureCN' ...
** testing if installed package can be loaded
* MD5 sums
packaged installation of 'PureCN' as PureCN_1.16.0.zip
* DONE (PureCN)
* installing to library 'C:/Users/biocbuild/bbs-3.10-bioc/R/library'
package 'PureCN' successfully unpacked and MD5 sums checked

Tests output

PureCN.Rcheck/tests_i386/testthat.Rout


R version 3.6.3 (2020-02-29) -- "Holding the Windsock"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: i386-w64-mingw32/i386 (32-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(PureCN)
Loading required package: DNAcopy
Loading required package: VariantAnnotation
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which, which.max, which.min

Loading required package: GenomeInfoDb
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:base':

    expand.grid

Loading required package: IRanges

Attaching package: 'IRanges'

The following object is masked from 'package:grDevices':

    windows

Loading required package: GenomicRanges
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: 'matrixStats'

The following objects are masked from 'package:Biobase':

    anyMissing, rowMedians

Loading required package: BiocParallel

Attaching package: 'DelayedArray'

The following objects are masked from 'package:matrixStats':

    colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges

The following objects are masked from 'package:base':

    aperm, apply, rowsum

Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit


Attaching package: 'VariantAnnotation'

The following object is masked from 'package:base':

    tabulate

> 
> test_check("PureCN")
WARN [2020-04-15 05:57:46] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
WARN [2020-04-15 05:57:46] Chromosome naming style of txdb file (UCSC) was different from interval file (NCBI).
WARN [2020-04-15 05:57:50] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
FATAL [2020-04-15 05:57:54] tumor.coverage.file and interval.file do not align. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] Need either f or purity and ploidy. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] f not in expected range. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] coverage not in expected range (>=2) 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] purity not in expected range. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] ploidy not in expected range. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:57:54] cell.fraction not in expected range. 

FATAL [2020-04-15 05:57:54]  

FATAL [2020-04-15 05:57:54] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:57:54] parameters (PureCN 1.16.0). 

INFO [2020-04-15 05:57:59] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 05:57:59] You are likely not using the correct baits file!
WARN [2020-04-15 05:57:59] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 05:57:59] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 05:57:59] Processing on-target regions...
INFO [2020-04-15 05:58:00] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 05:58:00] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 05:58:02] Tumor/normal noise ratio: 19.793
WARN [2020-04-15 05:58:02] Extensive noise in tumor compared to normals.
FATAL [2020-04-15 05:59:09] pvalue.cutoff not within expected range or format. 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:09] pvalue.cutoff not within expected range or format. 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:09] percentile.cutoff not in expected range (0 to 100). 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:09] percentile.cutoff not in expected range (0 to 100). 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:09] purity not within expected range or format. 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:09] purity not within expected range or format. 

FATAL [2020-04-15 05:59:09]  

FATAL [2020-04-15 05:59:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:09] parameters (PureCN 1.16.0). 

[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] CouldINFO [2020-04-15 05:59:12] ------------------------------------------------------------
INFO [2020-04-15 05:59:12] PureCN 1.16.0
INFO [2020-04-15 05:59:12] ------------------------------------------------------------
INFO [2020-04-15 05:59:12] Loading coverage files...
INFO [2020-04-15 05:59:13] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 05:59:13] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 05:59:13] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 05:59:13] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 05:59:13] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 05:59:13] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 05:59:13] No normalDB provided. Provide one for better results.
INFO [2020-04-15 05:59:13] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 05:59:13] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 05:59:13] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 05:59:13] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 05:59:13] Loading VCF...
INFO [2020-04-15 05:59:13] Found 127 variants in VCF file.
INFO [2020-04-15 05:59:14] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 05:59:14] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 05:59:14] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 05:59:15] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 05:59:16] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 05:59:16] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 05:59:16] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 05:59:16] 1.0% of targets contain variants.
INFO [2020-04-15 05:59:16] Removing 2 variants outside intervals.
INFO [2020-04-15 05:59:16] Found SOMATIC annotation in VCF.
INFO [2020-04-15 05:59:16] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 05:59:16] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 05:59:16] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 05:59:17] Sample sex: ?
INFO [2020-04-15 05:59:17] Segmenting data...
INFO [2020-04-15 05:59:17] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 05:59:17] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 05:59:19] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 05:59:19] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 05:59:19] Using 123 variants.
INFO [2020-04-15 05:59:19] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 05:59:19] 2D-grid search of purity and ploidy...
INFO [2020-04-15 05:59:22] Local optima: 0.63/1.9, 0.4/2.2, 0.5/2
INFO [2020-04-15 05:59:22] Testing local optimum 1/3 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 05:59:24] Testing local optimum 2/3 at purity 0.40 and total ploidy 2.20...
INFO [2020-04-15 05:59:26] Testing local optimum 3/3 at purity 0.50 and total ploidy 2.00...
INFO [2020-04-15 05:59:27] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 05:59:27] Fitting variants for local optimum 1/3...
INFO [2020-04-15 05:59:27] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 05:59:28] Optimized purity: 0.65
INFO [2020-04-15 05:59:28] Fitting variants for local optimum 2/3...
INFO [2020-04-15 05:59:28] Fitting variants for purity 0.40, tumor ploidy 2.34 and contamination 0.01.
INFO [2020-04-15 05:59:30] Optimized purity: 0.40
INFO [2020-04-15 05:59:30] Done.
INFO [2020-04-15 05:59:30] ------------------------------------------------------------
FATAL [2020-04-15 05:59:32] exclude not a GRanges object. 

FATAL [2020-04-15 05:59:32]  

FATAL [2020-04-15 05:59:32] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:32] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:32] callable not a GRanges object. 

FATAL [2020-04-15 05:59:32]  

FATAL [2020-04-15 05:59:32] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:32] parameters (PureCN 1.16.0). 

WARN [2020-04-15 05:59:45] tumor.coverage.file and interval.file do not align.
FATAL [2020-04-15 05:59:48] No gc_bias column in interval.file. 

FATAL [2020-04-15 05:59:48]  

FATAL [2020-04-15 05:59:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:48] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 05:59:49] Provided coverage is zero, most likely due to a corrupt BAM file. 

FATAL [2020-04-15 05:59:49]  

FATAL [2020-04-15 05:59:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:49] parameters (PureCN 1.16.0). 

INFO [2020-04-15 05:59:57] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:57] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:57] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:58] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:58] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:58] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
FATAL [2020-04-15 05:59:58] Purity or Ploidy not numeric or in expected range. 

FATAL [2020-04-15 05:59:58]  

FATAL [2020-04-15 05:59:58] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:58] parameters (PureCN 1.16.0). 

INFO [2020-04-15 05:59:58] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
INFO [2020-04-15 05:59:58] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.rds...
FATAL [2020-04-15 05:59:59] 'Failed' column in 

FATAL [2020-04-15 05:59:59] C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c9075357.csv 

FATAL [2020-04-15 05:59:59] not logical(1). 

FATAL [2020-04-15 05:59:59]  

FATAL [2020-04-15 05:59:59] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 05:59:59] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:00:00] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:00:00] You are likely not using the correct baits file!
WARN [2020-04-15 06:00:00] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:00] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:00] Processing on-target regions...
INFO [2020-04-15 06:00:01] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:00:01] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:00:06] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:00:06] You are likely not using the correct baits file!
WARN [2020-04-15 06:00:06] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:06] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:06] Processing on-target regions...
INFO [2020-04-15 06:00:07] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:00:07] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:00:12] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:00:12] You are likely not using the correct baits file!
WARN [2020-04-15 06:00:12] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:12] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:12] Processing on-target regions...
INFO [2020-04-15 06:00:13] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:00:13] Removing 1 intervals with zero coverage in more than 3% of normalDB.
WARN [2020-04-15 06:00:14] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:14] Sample sex: NA
WARN [2020-04-15 06:00:15] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:15] Sample sex: NA
INFO [2020-04-15 06:00:16] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:00:16] You are likely not using the correct baits file!
WARN [2020-04-15 06:00:17] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:17] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:00:17] Length of normal.coverage.files and sex different 

FATAL [2020-04-15 06:00:17]  

FATAL [2020-04-15 06:00:17] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:17] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:00:22] Target intervals were not sorted.
INFO [2020-04-15 06:00:22] 560 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:00:22] You are likely not using the correct baits file!
WARN [2020-04-15 06:00:22] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:22] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:22] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:22] Processing on-target regions...
INFO [2020-04-15 06:00:22] Removing 978 intervals with low coverage in normalDB.
INFO [2020-04-15 06:00:22] Removing 11 intervals with zero coverage in more than 3% of normalDB.
FATAL [2020-04-15 06:00:25] tumor.coverage.file and normalDB do not align. 

FATAL [2020-04-15 06:00:25]  

FATAL [2020-04-15 06:00:25] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:25] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:00:25] At least 2 normal.coverage.files required. 

FATAL [2020-04-15 06:00:25]  

FATAL [2020-04-15 06:00:25] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:25] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:00:30] ------------------------------------------------------------
INFO [2020-04-15 06:00:30] PureCN 1.16.0
INFO [2020-04-15 06:00:30] ------------------------------------------------------------
INFO [2020-04-15 06:00:30] Loading coverage files...
INFO [2020-04-15 06:00:31] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:00:31] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:31] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:31] Removing 228 intervals with missing log.ratio.
FATAL [2020-04-15 06:00:31] normalDB incompatible with this PureCN version. Please re-run 

FATAL [2020-04-15 06:00:31] NormalDB.R. 

FATAL [2020-04-15 06:00:31]  

FATAL [2020-04-15 06:00:31] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:31] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:00:31] normalDB incompatible with this PureCN version. Please re-run 

FATAL [2020-04-15 06:00:31] NormalDB.R. 

FATAL [2020-04-15 06:00:31]  

FATAL [2020-04-15 06:00:31] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:31] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:00:32] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:33] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:33] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:33] Removing 16 low quality variants with BQ < 25.
INFO [2020-04-15 06:00:33] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:34] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:35] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:35] Removing 6 blacklisted variants.
INFO [2020-04-15 06:00:35] Removing 16 low quality variants with BQ < 25.
INFO [2020-04-15 06:00:35] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:36] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:36] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:37] Removing 16 low quality variants with BQ < 25.
WARN [2020-04-15 06:00:37] MuTect stats file lacks contig and position columns.
INFO [2020-04-15 06:00:37] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:38] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:38] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:38] Removing 16 low quality variants with BQ < 25.
WARN [2020-04-15 06:00:39] MuTect stats file and VCF file do not align perfectly. Will remove 2325 unmatched variants.
WARN [2020-04-15 06:00:39] MuTect stats file lacks failure_reasons column. Keeping all variants listed in stats file.
INFO [2020-04-15 06:00:39] Removing 0 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:40] Removing 0 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:40] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:00:40] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:41] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:41] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:42] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:00:42] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:00:42] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:00:43] Found 11 variants in VCF file.
WARN [2020-04-15 06:00:44] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2020-04-15 06:00:44] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:00:49] Found 11 variants in VCF file.
WARN [2020-04-15 06:00:50] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2020-04-15 06:00:50] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:00:52] Found 11 variants in VCF file.
WARN [2020-04-15 06:00:52] vcf.file has no DB or POP_AF info field for membership in germline databases. Guessing it based on available dbSNP ID.
INFO [2020-04-15 06:00:53] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:00:54] Found 1000 variants in VCF file.
INFO [2020-04-15 06:00:54] Removing 2 triallelic sites.
WARN [2020-04-15 06:00:54] DP FORMAT field contains NAs. Removing 44 variants.
INFO [2020-04-15 06:00:54] 954 (100.0%) variants annotated as likely germline (DB INFO flag).
WARN [2020-04-15 06:00:57] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:00:57] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:00:57] Mean coverages: chrX: 108.58, chrY: 108.58, chr1-22: 104.87.
INFO [2020-04-15 06:00:57] Mean coverages: chrX: 108.58, chrY: 2.17, chr1-22: 104.87.
INFO [2020-04-15 06:00:57] Mean coverages: chrX: 108.58, chrY: 5.17, chr1-22: 104.87.
 not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the headINFO [2020-04-15 06:00:58] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:00:58] No germline variants in VCF.
FATAL [2020-04-15 06:00:58] No solution with id hello 

FATAL [2020-04-15 06:00:58]  

FATAL [2020-04-15 06:00:58] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:58] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:00:58] No solution with id 100 

FATAL [2020-04-15 06:00:58]  

FATAL [2020-04-15 06:00:58] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:00:58] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:01:00] all.data and w have different lengths. 

FATAL [2020-04-15 06:01:00]  

FATAL [2020-04-15 06:01:00] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:01:00] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:01:02] No mappability scores provided.
WARN [2020-04-15 06:01:02] No reptiming scores provided.
INFO [2020-04-15 06:01:02] Calculating GC-content...
WARN [2020-04-15 06:01:02] No mappability scores provided.
WARN [2020-04-15 06:01:02] No reptiming scores provided.
INFO [2020-04-15 06:01:02] Calculating GC-content...
INFO [2020-04-15 06:01:02] Removing 1 targets overlapping with exclude.
WARN [2020-04-15 06:01:02] No mappability scores provided.
WARN [2020-04-15 06:01:02] No reptiming scores provided.
INFO [2020-04-15 06:01:02] Calculating GC-content...
WARN [2020-04-15 06:01:03] Found small target regions (< 100bp). Will resize them.
FATAL [2020-04-15 06:01:03] off.target.padding must be negative. 

FATAL [2020-04-15 06:01:03]  

FATAL [2020-04-15 06:01:03] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:01:03] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:01:03] Interval coordinates should start at 1, not at 0 

FATAL [2020-04-15 06:01:03]  

FATAL [2020-04-15 06:01:03] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:01:03] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:01:03] Found small target regions (< 100bp). Will resize them.
FATAL [2020-04-15 06:01:04] No off-target regions after filtering for mappability and 

FATAL [2020-04-15 06:01:04] off.target.padding 

FATAL [2020-04-15 06:01:04]  

FATAL [2020-04-15 06:01:04] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:01:04] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:01:04] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:01:04] No mappability scores provided.
INFO [2020-04-15 06:01:04] Calculating GC-content...
WARN [2020-04-15 06:01:04] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:01:05] Averaging reptiming into bins of size 200...
WARN [2020-04-15 06:01:05] No mappability scores provided.
INFO [2020-04-15 06:01:05] Calculating GC-content...
WARN [2020-04-15 06:01:05] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:01:05] Splitting 1 large targets to an average width of 200.
WARN [2020-04-15 06:01:05] No mappability scores provided.
WARN [2020-04-15 06:01:05] No reptiming scores provided.
INFO [2020-04-15 06:01:05] Calculating GC-content...
WARN [2020-04-15 06:01:06] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:01:06] No mappability scores provided.
WARN [2020-04-15 06:01:06] No reptiming scores provided.
INFO [2020-04-15 06:01:06] Calculating GC-content...
INFO [2020-04-15 06:01:06] Tiling off-target regions to an average width of 200000.
WARN [2020-04-15 06:01:07] No mappability scores provided.
WARN [2020-04-15 06:01:07] No reptiming scores provided.
INFO [2020-04-15 06:01:07] Calculating GC-content...
WARN [2020-04-15 06:01:07] Intervals contain off-target regions. Will not change intervals.
WARN [2020-04-15 06:01:07] No mappability scores provided.
WARN [2020-04-15 06:01:07] No reptiming scores provided.
INFO [2020-04-15 06:01:07] Calculating GC-content...
WARN [2020-04-15 06:01:07] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:01:07] No reptiming scores provided.
INFO [2020-04-15 06:01:07] Calculating GC-content...
WARN [2020-04-15 06:01:09] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:01:09] 1 intervals without mappability score (1 on-target).
INFO [2020-04-15 06:01:09] Removing 1 intervals with low mappability score (<0.50).
WARN [2020-04-15 06:01:09] No reptiming scores provided.
INFO [2020-04-15 06:01:09] Calculating GC-content...
WARN [2020-04-15 06:01:09] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:01:10] Tiling off-target regions to an average width of 200000.
WARN [2020-04-15 06:01:10] No reptiming scores provided.
INFO [2020-04-15 06:01:10] Calculating GC-content...
WARN [2020-04-15 06:01:11] No mappability scores provided.
WARN [2020-04-15 06:01:11] No reptiming scores provided.
INFO [2020-04-15 06:01:11] Calculating GC-content...
WARN [2020-04-15 06:01:11] Chromosome naming style of interval file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:01:11] No mappability scores provided.
WARN [2020-04-15 06:01:11] No reptiming scores provided.
INFO [2020-04-15 06:01:11] Calculating GC-content...
FATAL [2020-04-15 06:01:11] Chromosome naming style of interval file unknown, should be UCSC. 

FATAL [2020-04-15 06:01:11]  

FATAL [2020-04-15 06:01:11] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:01:11] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:01:11] Chromosome naming style of interval file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:01:11] Chromosome naming style of mappability file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:01:11] No reptiming scores provided.
INFO [2020-04-15 06:01:11] Calculating GC-content...
WARN [2020-04-15 06:01:12] Found small target regions (< 60bp). Will resize them.
WARN [2020-04-15 06:01:12] No mappability scores provided.
WARN [2020-04-15 06:01:12] No reptiming scores provided.
INFO [2020-04-15 06:01:12] Calculating GC-content...
WARN [2020-04-15 06:01:12] Found small target regions (< 60bp). Will drop them.
WARN [2020-04-15 06:01:12] No mappability scores provided.
WARN [2020-04-15 06:01:12] No reptiming scores provided.
INFO [2020-04-15 06:01:12] Calculating GC-content...
WARN [2020-04-15 06:01:12] Found small target regions (< 200bp). Will resize them.
WARN [2020-04-15 06:01:12] No mappability scores provided.
WARN [2020-04-15 06:01:12] No reptiming scores provided.
INFO [2020-04-15 06:01:12] Calculating GC-content...
INFO [2020-04-15 06:01:13] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:01:13] You are likely not using the correct baits file!
WARN [2020-04-15 06:01:13] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:01:13] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:01:13] Processing on-target regions...
INFO [2020-04-15 06:01:13] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:01:13] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:01:17] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p 
multipcf finished for chromosome arm 1q 
multipcf finished for chromosome arm 2p 
multipcf finished for chromosome arm 2q 
multipcf finished for chromosome arm 3p 
multipcf finished for chromosome arm 3q 
multipcf finished for chromosome arm 4p 
multipcf finished for chromosome arm 4q 
multipcf finished for chromosome arm 5p 
multipcf finished for chromosome arm 5q 
multipcf finished for chromosome arm 6p 
multipcf finished for chromosome arm 6q 
multipcf finished for chromosome arm 7p 
multipcf finished for chromosome arm 7q 
multipcf finished for chromosome arm 8p 
multipcf finished for chromosome arm 8q 
multipcf finished for chromosome arm 9p 
multipcf finished for chromosome arm 9q 
multipcf finished for chromosome arm 10p 
multipcf finished for chromosome arm 10q 
multipcf finished for chromosome arm 11p 
multipcf finished for chromosome arm 11q 
multipcf finished for chromosome arm 12p 
multipcf finished for chromosome arm 12q 
multipcf finished for chromosome arm 13q 
multipcf finished for chromosome arm 14q 
multipcf finished for chromosome arm 15q 
multipcf finished for chromosome arm 16p 
multipcf finished for chromosome arm 16q 
multipcf finished for chromosome arm 17p 
multipcf finished for chromosome arm 17q 
multipcf finished for chromosome arm 18p 
multipcf finished for chromosome arm 18q 
multipcf finished for chromosome arm 19p 
multipcf finished for chromosome arm 19q 
multipcf finished for chromosome arm 20p 
multipcf finished for chromosome arm 20q 
multipcf finished for chromosome arm 21q 
multipcf finished for chromosome arm 22q 
INFO [2020-04-15 06:01:19] ------------------------------------------------------------
INFO [2020-04-15 06:01:19] PureCN 1.16.0
INFO [2020-04-15 06:01:19] ------------------------------------------------------------
INFO [2020-04-15 06:01:19] Loading coverage files...
INFO [2020-04-15 06:01:19] seg.file and normal.coverage.file provided. Using both.
INFO [2020-04-15 06:01:19] Mean target coverages: 112X (tumor) 105X (normal).
WARN [2020-04-15 06:01:19] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:01:19] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:01:19] Removing 199 intervals with missing log.ratio.
INFO [2020-04-15 06:01:19] Using 9850 intervals (9850 on-target, 0 off-target).
INFO [2020-04-15 06:01:19] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:01:19] Loading VCF...
er line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2INFO [2020-04-15 06:01:20] Found 127 variants in VCF file.
INFO [2020-04-15 06:01:20] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:01:20] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:01:20] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:01:20] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:01:21] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:01:21] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:01:21] Total size of targeted genomic region: 1.68Mb (2.66Mb with 50bp padding).
INFO [2020-04-15 06:01:21] 1.0% of targets contain variants.
INFO [2020-04-15 06:01:21] Removing 0 variants outside intervals.
INFO [2020-04-15 06:01:21] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:01:21] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:01:21] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2020-04-15 06:01:21] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:01:21] Sample sex: ?
INFO [2020-04-15 06:01:21] Segmenting data...
INFO [2020-04-15 06:01:21] Loaded provided segmentation file file308c32f32bc0.seg (format DNAcopy).
INFO [2020-04-15 06:01:21] Re-centering provided segment means (offset 0.0110).
INFO [2020-04-15 06:01:21] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:01:22] Found 59 segments with median size of 30.15Mb.
INFO [2020-04-15 06:01:22] Using 125 variants.
INFO [2020-04-15 06:01:22] Mean standard deviation of log-ratios: 0.34
INFO [2020-04-15 06:01:22] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:01:23] Local optima: 0.6/1.9, 0.4/2.1
INFO [2020-04-15 06:01:23] Testing local optimum 1/2 at purity 0.60 and total ploidy 1.90...
INFO [2020-04-15 06:01:24] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:01:25] Recalibrating log-ratios...
INFO [2020-04-15 06:01:25] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:01:25] Recalibrating log-ratios...
INFO [2020-04-15 06:01:25] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:01:25] Recalibrating log-ratios...
INFO [2020-04-15 06:01:25] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:01:26] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:01:26] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:01:26] Fitting variants for purity 0.60, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:27] Fitting variants for purity 0.40, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:28] Fitting variants for purity 0.45, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:29] Fitting variants for purity 0.50, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:31] Fitting variants for purity 0.55, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:32] Fitting variants for purity 0.65, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:33] Fitting variants for purity 0.70, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:01:34] Optimized purity: 0.65
INFO [2020-04-15 06:01:34] Done.
INFO [2020-04-15 06:01:34] ------------------------------------------------------------
INFO [2020-04-15 06:01:37] Loaded provided segmentation file example_seg.txt (format DNAcopy).
INFO [2020-04-15 06:01:37] Re-centering provided segment means (offset -0.0033).
INFO [2020-04-15 06:01:37] Loaded provided segmentation file example_gatk4_modelfinal.seg.gz (format GATK4).
WARN [2020-04-15 06:01:37] Expecting numeric chromosome names in seg.file, assuming file is properly sorted.
INFO [2020-04-15 06:01:37] Re-centering provided segment means (offset -0.0037).
INFO [2020-04-15 06:01:38] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:01:38] You are likely not using the correct baits file!
WARN [2020-04-15 06:01:38] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:01:38] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:01:38] Processing on-target regions...
INFO [2020-04-15 06:01:38] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:01:38] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:01:40] ------------------------------------------------------------
INFO [2020-04-15 06:01:40] PureCN 1.16.0
INFO [2020-04-15 06:01:40] ------------------------------------------------------------
INFO [2020-04-15 06:01:41] Using BiocParallel for parallel optimization.
INFO [2020-04-15 06:01:41] Loading coverage files...
INFO [2020-04-15 06:01:42] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:01:42] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:01:42] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:01:42] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:01:42] Removing 705 intervals excluded in normalDB.
INFO [2020-04-15 06:01:42] normalDB provided. Setting minimum coverage for segmentation to 0.0015X.
INFO [2020-04-15 06:01:42] Using 9116 intervals (9116 on-target, 0 off-target).
INFO [2020-04-15 06:01:42] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:01:42] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:01:42] Sample sex: ?
INFO [2020-04-15 06:01:42] Segmenting data...
INFO [2020-04-15 06:01:42] Interval weights found, will use weighted CBS.
INFO [2020-04-15 06:01:42] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:01:42] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:01:44] Found 48 segments with median size of 31.71Mb.
INFO [2020-04-15 06:01:44] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:01:44] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:01:44] Local optima: 0.62/1.9, 0.38/2.2, 0.31/1.9, 0.27/2, 0.78/2
INFO [2020-04-15 06:02:40] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:02:40] Skipping 3 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:02:40] Done.
INFO [2020-04-15 06:02:40] ------------------------------------------------------------
INFO [2020-04-15 06:02:40] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\Rtmpe0WONQ\file308c2def63c.rds...
FATAL [2020-04-15 06:02:40] runAbsoluteCN was run without a VCF file. 

FATAL [2020-04-15 06:02:40]  

FATAL [2020-04-15 06:02:40] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:40] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:02:40] runAbsoluteCN was run without a VCF file. 

FATAL [2020-04-15 06:02:40]  

FATAL [2020-04-15 06:02:40] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:40] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:40] ------------------------------------------------------------
INFO [2020-04-15 06:02:40] PureCN 1.16.0
INFO [2020-04-15 06:02:40] ------------------------------------------------------------
INFO [2020-04-15 06:02:40] Loading coverage files...
FATAL [2020-04-15 06:02:41] Need a normal coverage file if log.ratio and seg.file are not provided. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
FATAL [2020-04-15 06:02:41] min.ploidy or max.ploidy not within expected range. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
FATAL [2020-04-15 06:02:41] min.ploidy or max.ploidy not within expected range. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
FATAL [2020-04-15 06:02:41] test.num.copy not within expected range. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
WARN [2020-04-15 06:02:41] test.num.copy outside recommended range.
FATAL [2020-04-15 06:02:41] max.non.clonal not within expected range or format. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
WARN [2020-04-15 06:02:41] test.num.copy outside recommended range.
FATAL [2020-04-15 06:02:41] max.non.clonal not within expected range or format. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
FATAL [2020-04-15 06:02:41] test.purity not within expected range. 

FATAL [2020-04-15 06:02:41]  

FATAL [2020-04-15 06:02:41] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:41] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] PureCN 1.16.0
INFO [2020-04-15 06:02:41] ------------------------------------------------------------
INFO [2020-04-15 06:02:41] Loading coverage files...
FATAL [2020-04-15 06:02:42] Tumor and normal are identical. This won't give any meaningful results 

FATAL [2020-04-15 06:02:42] and I'm stopping here. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] Loading coverage files...
FATAL [2020-04-15 06:02:42] Length of log.ratio different from tumor coverage. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] prior.purity must have the same length as test.purity. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] min.gof not within expected range or format. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] prior.purity not within expected range or format. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] prior.purity must add to 1. Sum is 1.5 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] max.homozygous.loss not within expected range or format. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] prior.K not within expected range or format. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] prior.contamination not within expected range or format. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] Iterations not in the expected range from 10 to 250. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
FATAL [2020-04-15 06:02:42] Iterations not in the expected range from 10 to 250. 

FATAL [2020-04-15 06:02:42]  

FATAL [2020-04-15 06:02:42] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:42] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] PureCN 1.16.0
INFO [2020-04-15 06:02:42] ------------------------------------------------------------
INFO [2020-04-15 06:02:42] Loading coverage files...
FATAL [2020-04-15 06:02:43] Missing tumor.coverage.file requires seg.file or log.ratio and 

FATAL [2020-04-15 06:02:43] interval.file. 

FATAL [2020-04-15 06:02:43]  

FATAL [2020-04-15 06:02:43] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:43] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:43] ------------------------------------------------------------
INFO [2020-04-15 06:02:43] PureCN 1.16.0
INFO [2020-04-15 06:02:43] ------------------------------------------------------------
INFO [2020-04-15 06:02:43] ------------------------------------------------------------
INFO [2020-04-15 06:02:43] PureCN 1.16.0
INFO [2020-04-15 06:02:43] ------------------------------------------------------------
INFO [2020-04-15 06:02:43] Loading coverage files...
FATAL [2020-04-15 06:02:44] Interval files in normal and tumor different. 

FATAL [2020-04-15 06:02:44]  

FATAL [2020-04-15 06:02:44] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:44] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:44] ------------------------------------------------------------
INFO [2020-04-15 06:02:44] PureCN 1.16.0
INFO [2020-04-15 06:02:44] ------------------------------------------------------------
INFO [2020-04-15 06:02:44] Loading coverage files...
INFO [2020-04-15 06:02:45] Mean target coverages: 0X (tumor) 99X (normal).
WARN [2020-04-15 06:02:45] Large difference in coverage of tumor and normal.
FATAL [2020-04-15 06:02:45] No finite intervals. 

FATAL [2020-04-15 06:02:45]  

FATAL [2020-04-15 06:02:45] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:45] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:45] ------------------------------------------------------------
INFO [2020-04-15 06:02:45] PureCN 1.16.0
INFO [2020-04-15 06:02:45] ------------------------------------------------------------
INFO [2020-04-15 06:02:45] Loading coverage files...
INFO [2020-04-15 06:02:45] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:02:46] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:02:46] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:02:46] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:02:46] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:02:46] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:02:46] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:02:46] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:02:46] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:02:46] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:02:46] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:02:46] Loading VCF...
2,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""INFO [2020-04-15 06:02:46] Found 127 variants in VCF file.
INFO [2020-04-15 06:02:46] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:02:46] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:02:47] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:02:47] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:02:48] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
Error in FileForFormat(con) : Format 'txt' unsupported
FATAL [2020-04-15 06:02:48] Could not import snp.blacklist 

FATAL [2020-04-15 06:02:48] C:/Users/biocbuild/bbs-3.10-bioc/R/library/PureCN/extdata/example_normal.txt:Error 

FATAL [2020-04-15 06:02:48] in FileForFormat(con) : Format 'txt' unsupported 

FATAL [2020-04-15 06:02:48]  

FATAL [2020-04-15 06:02:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:02:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:02:48] ------------------------------------------------------------
INFO [2020-04-15 06:02:48] PureCN 1.16.0
INFO [2020-04-15 06:02:48] ------------------------------------------------------------
INFO [2020-04-15 06:02:48] Loading coverage files...
INFO [2020-04-15 06:02:48] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:02:49] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:02:49] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:02:49] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:02:49] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:02:49] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:02:49] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:02:49] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:02:49] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:02:49] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:02:49] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:02:49] Loading VCF...
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_INFO [2020-04-15 06:02:49] Found 127 variants in VCF file.
INFO [2020-04-15 06:02:49] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:02:49] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:02:49] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:02:50] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:02:51] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:02:51] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:02:51] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:02:51] 1.0% of targets contain variants.
INFO [2020-04-15 06:02:51] Removing 2 variants outside intervals.
INFO [2020-04-15 06:02:51] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:02:51] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:02:51] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:02:51] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:02:51] Sample sex: ?
INFO [2020-04-15 06:02:51] Segmenting data...
INFO [2020-04-15 06:02:51] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:02:51] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:02:54] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:02:54] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:02:54] Using 123 variants.
INFO [2020-04-15 06:02:54] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:02:54] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:02:56] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2020-04-15 06:02:56] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:02:58] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:02:58] Recalibrating log-ratios...
INFO [2020-04-15 06:02:58] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:02:59] Recalibrating log-ratios...
INFO [2020-04-15 06:02:59] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:00] Recalibrating log-ratios...
INFO [2020-04-15 06:03:00] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:00] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:01] Recalibrating log-ratios...
INFO [2020-04-15 06:03:01] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:01] Recalibrating log-ratios...
INFO [2020-04-15 06:03:01] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:02] Recalibrating log-ratios...
INFO [2020-04-15 06:03:02] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:02] Skipping 2 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:03:02] Fitting variants for local optimum 1/3...
INFO [2020-04-15 06:03:02] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:03:04] Optimized purity: 0.65
INFO [2020-04-15 06:03:04] Done.
INFO [2020-04-15 06:03:04] ------------------------------------------------------------
parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parINFO [2020-04-15 06:03:04] ------------------------------------------------------------
INFO [2020-04-15 06:03:04] PureCN 1.16.0
INFO [2020-04-15 06:03:04] ------------------------------------------------------------
INFO [2020-04-15 06:03:04] Loading coverage files...
INFO [2020-04-15 06:03:05] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:03:05] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:03:05] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:03:05] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:03:05] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:03:05] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:03:05] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:03:05] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:03:05] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:03:05] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:03:05] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:03:05] Loading VCF...
INFO [2020-04-15 06:03:05] Found 127 variants in VCF file.
WARN [2020-04-15 06:03:05] DP FORMAT field contains NAs. Removing 3 variants.
WARN [2020-04-15 06:03:05] DB INFO flag contains NAs
INFO [2020-04-15 06:03:05] 119 (96.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:03:06] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:03:06] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:03:08] Removing 1 variants with AF < 0.030 or AF >= 0.970 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:03:08] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:03:08] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:03:08] 1.0% of targets contain variants.
INFO [2020-04-15 06:03:08] Setting somatic prior probabilities for dbSNP hits to 0.000500 or to 0.500000 otherwise.
INFO [2020-04-15 06:03:08] Excluding 5 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:03:08] Sample sex: ?
INFO [2020-04-15 06:03:08] Segmenting data...
INFO [2020-04-15 06:03:08] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:03:08] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:03:10] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:03:10] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:03:10] Using 123 variants.
INFO [2020-04-15 06:03:10] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:03:10] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:03:11] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2020-04-15 06:03:11] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:03:13] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:13] Recalibrating log-ratios...
INFO [2020-04-15 06:03:13] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:13] Recalibrating log-ratios...
INFO [2020-04-15 06:03:13] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:14] Recalibrating log-ratios...
INFO [2020-04-15 06:03:14] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:03:14] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:15] Recalibrating log-ratios...
INFO [2020-04-15 06:03:15] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:15] Recalibrating log-ratios...
INFO [2020-04-15 06:03:15] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:16] Recalibrating log-ratios...
INFO [2020-04-15 06:03:16] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:03:16] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:03:16] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:03:16] Fitting variants for local optimum 1/3...
INFO [2020-04-15 06:03:16] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:03:18] Optimized purity: 0.65
INFO [2020-04-15 06:03:18] Done.
INFO [2020-04-15 06:03:18] ------------------------------------------------------------
INFO [2020-04-15 06:03:18] ------------------------------------------------------------
INFO [2020-04-15 06:03:18] PureCN 1.16.0
INFO [2020-04-15 06:03:18] ------------------------------------------------------------
INFO [2020-04-15 06:03:18] Loading coverage files...
INFO [2020-04-15 06:03:19] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:03:19] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:03:19] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:03:19] No Gene column in interval.file. You won't get gene-level calls.
INFO [2020-04-15 06:03:19] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:03:20] Removing 15 low/high GC targets.
INFO [2020-04-15 06:03:20] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:03:20] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:03:20] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:03:20] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:03:20] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:03:20] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:03:20] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:03:20] Loading VCF...
se the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=INFO [2020-04-15 06:03:20] Found 127 variants in VCF file.
INFO [2020-04-15 06:03:20] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:03:20] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:03:21] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:03:21] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:03:22] Removing 1 variants with AF < 0.030 or AF >= Inf or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:03:22] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:03:22] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:03:22] 1.0% of targets contain variants.
INFO [2020-04-15 06:03:22] Removing 2 variants outside intervals.
INFO [2020-04-15 06:03:22] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:03:22] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:03:23] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:03:23] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:03:23] Sample sex: ?
INFO [2020-04-15 06:03:23] Segmenting data...
INFO [2020-04-15 06:03:23] Using unweighted PSCBS.
INFO [2020-04-15 06:03:23] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:04:37] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:04:37] Found 72 segments with median size of 26.71Mb.
INFO [2020-04-15 06:04:37] Using 123 variants.
INFO [2020-04-15 06:04:37] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:04:37] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:04:37] Local optima: 0.62/1.9, 0.38/2.2, 0.31/1.9, 0.27/2, 0.78/2
INFO [2020-04-15 06:04:37] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:04:37] Recalibrating log-ratios...
INFO [2020-04-15 06:04:37] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:04:38] Recalibrating log-ratios...
INFO [2020-04-15 06:04:38] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:04:38] Recalibrating log-ratios...
INFO [2020-04-15 06:04:38] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:04:38] Testing local optimum 2/5 at purity 0.38 and total ploidy 2.20...
INFO [2020-04-15 06:04:40] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:04:41] Recalibrating log-ratios...
INFO [2020-04-15 06:04:41] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:04:41] Recalibrating log-ratios...
INFO [2020-04-15 06:04:41] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:04:42] Recalibrating log-ratios...
INFO [2020-04-15 06:04:42] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:04:42] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:04:43] Recalibrating log-ratios...
INFO [2020-04-15 06:04:43] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:04:43] Recalibrating log-ratios...
INFO [2020-04-15 06:04:43] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:04:43] Recalibrating log-ratios...
INFO [2020-04-15 06:04:43] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:04:44] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:04:44] Recalibrating log-ratios...
INFO [2020-04-15 06:04:44] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:04:45] Recalibrating log-ratios...
INFO [2020-04-15 06:04:45] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:04:45] Recalibrating log-ratios...
INFO [2020-04-15 06:04:45] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:04:45] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:04:45] Skipping 3 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:04:45] Fitting variants for local optimum 2/5...
INFO [2020-04-15 06:04:45] Fitting variants for purity 0.40, tumor ploidy 2.55 and contamination 0.01.
INFO [2020-04-15 06:04:47] Optimized purity: 0.40
INFO [2020-04-15 06:04:47] Done.
INFO [2020-04-15 06:04:47] ------------------------------------------------------------
FATAL [2020-04-15 06:04:47] This function requires gene-level calls. Please add a column 'Gene' 

FATAL [2020-04-15 06:04:47] containing gene symbols to the interval.file. 

FATAL [2020-04-15 06:04:47]  

FATAL [2020-04-15 06:04:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:04:47] parameters (PureCN 1.16.0). 

<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=INFO [2020-04-15 06:04:48] ------------------------------------------------------------
INFO [2020-04-15 06:04:48] PureCN 1.16.0
INFO [2020-04-15 06:04:48] ------------------------------------------------------------
INFO [2020-04-15 06:04:48] Loading coverage files...
INFO [2020-04-15 06:04:48] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:04:48] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:04:48] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:04:48] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:04:48] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:04:48] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:04:48] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:04:48] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:04:48] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:04:48] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:04:48] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:04:48] Loading VCF...
INFO [2020-04-15 06:04:48] Found 127 variants in VCF file.
INFO [2020-04-15 06:04:49] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
FATAL [2020-04-15 06:04:49] Different chromosome names in coverage and VCF. 

FATAL [2020-04-15 06:04:49]  

FATAL [2020-04-15 06:04:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:04:49] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:04:49] ------------------------------------------------------------
INFO [2020-04-15 06:04:49] PureCN 1.16.0
INFO [2020-04-15 06:04:49] ------------------------------------------------------------
INFO [2020-04-15 06:04:49] Loading coverage files...
INFO [2020-04-15 06:04:49] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:04:49] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:04:49] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:04:49] tumor.coverage.file and interval.file do not align. 

FATAL [2020-04-15 06:04:49]  

FATAL [2020-04-15 06:04:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:04:49] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:05:21] ------------------------------------------------------------
INFO [2020-04-15 06:05:21] PureCN 1.16.0
INFO [2020-04-15 06:05:21] ------------------------------------------------------------
INFO [2020-04-15 06:05:56] Loading coverage files...
INFO [2020-04-15 06:05:56] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:05:57] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:05:57] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:05:58] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:05:58] Removing 15 low/high GC targets.
INFO [2020-04-15 06:05:58] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:05:58] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:05:58] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:05:58] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:05:58] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:05:58] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:05:58] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:05:58] Loading VCF...
INFO [2020-04-15 06:05:58] Found 127 variants in VCF file.
INFO [2020-04-15 06:05:58] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:05:58] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:05:58] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:05:58] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:05:59] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:05:59] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:05:59] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:05:59] 1.0% of targets contain variants.
INFO [2020-04-15 06:05:59] Removing 2 variants outside intervals.
INFO [2020-04-15 06:05:59] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:05:59] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:05:59] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:05:59] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:05:59] Sample sex: ?
INFO [2020-04-15 06:05:59] Segmenting data...
INFO [2020-04-15 06:05:59] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:05:59] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:06:00] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:06:01] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:06:01] Using 123 variants.
INFO [2020-04-15 06:06:01] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:06:01] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:06:01] Local optima: 0.63/1.9, 0.4/2.1
INFO [2020-04-15 06:06:01] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 06:06:02] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:03] Recalibrating log-ratios...
INFO [2020-04-15 06:06:03] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:03] Recalibrating log-ratios...
INFO [2020-04-15 06:06:03] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:03] Recalibrating log-ratios...
INFO [2020-04-15 06:06:03] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:04] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:06:04] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:06:04] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:06:05] Optimized purity: 0.65
INFO [2020-04-15 06:06:05] Done.
INFO [2020-04-15 06:06:05] ------------------------------------------------------------
INFO [2020-04-15 06:06:05] ------------------------------------------------------------
INFO [2020-04-15 06:06:05] PureCN 1.16.0
INFO [2020-04-15 06:06:05] ------------------------------------------------------------
INFO [2020-04-15 06:06:05] Loading coverage files...
INFO [2020-04-15 06:06:05] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:06:05] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:06:05] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:06:06] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:06:06] Removing 15 low/high GC targets.
INFO [2020-04-15 06:06:06] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:06:06] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:06:06] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:06:06] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:06:06] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:06:06] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:06:06] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:06:06] Loading VCF...
INFO [2020-04-15 06:06:06] Found 127 variants in VCF file.
INFO [2020-04-15 06:06:06] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:06:06] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:06:07] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:06:07] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:06:07] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:06:07] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:06:07] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:06:07] 1.0% of targets contain variants.
INFO [2020-04-15 06:06:07] Removing 2 variants outside intervals.
INFO [2020-04-15 06:06:07] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:06:07] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:06:07] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:06:07] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:06:07] Sample sex: ?
INFO [2020-04-15 06:06:07] Segmenting data...
INFO [2020-04-15 06:06:08] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:06:08] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:06:09] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:06:09] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:06:09] Using 123 variants.
INFO [2020-04-15 06:06:09] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:06:09] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:06:10] Local optima: 0.63/1.9, 0.4/2.1
INFO [2020-04-15 06:06:10] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 06:06:11] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:11] Recalibrating log-ratios...
INFO [2020-04-15 06:06:11] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:12] Recalibrating log-ratios...
INFO [2020-04-15 06:06:12] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:12] Recalibrating log-ratios...
INFO [2020-04-15 06:06:12] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:06:12] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:06:12] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:06:13] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:06:14] Optimized purity: 0.65
INFO [2020-04-15 06:06:14] Done.
INFO [2020-04-15 06:06:14] ------------------------------------------------------------
FATAL [2020-04-15 06:06:56] chr1 not valid chromosome name(s). Valid names are: 

FATAL [2020-04-15 06:06:56] 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22 

FATAL [2020-04-15 06:06:56]  

FATAL [2020-04-15 06:06:56] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:06:56] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:07:02] ------------------------------------------------------------
INFO [2020-04-15 06:07:02] PureCN 1.16.0
INFO [2020-04-15 06:07:02] ------------------------------------------------------------
INFO [2020-04-15 06:07:02] Loading coverage files...
WARN [2020-04-15 06:07:02] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
WARN [2020-04-15 06:07:02] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:02] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:07:02] Removing 10 intervals with missing log.ratio.
INFO [2020-04-15 06:07:02] Using 10039 intervals (10039 on-target, 0 off-target).
INFO [2020-04-15 06:07:02] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:07:02] Loading VCF...
90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
INFO [2020-04-15 06:07:02] Found 127 variants in VCF file.
INFO [2020-04-15 06:07:02] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:07:03] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:07:03] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:07:03] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:07:03] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:07:03] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:07:03] Total size of targeted genomic region: 1.71Mb (2.70Mb with 50bp padding).
INFO [2020-04-15 06:07:04] 1.0% of targets contain variants.
INFO [2020-04-15 06:07:04] Removing 0 variants outside intervals.
INFO [2020-04-15 06:07:04] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:07:04] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:07:04] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2020-04-15 06:07:04] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:07:04] Sample sex: ?
INFO [2020-04-15 06:07:04] Segmenting data...
INFO [2020-04-15 06:07:04] Loaded provided segmentation file example_seg.txt (format DNAcopy).
WARN [2020-04-15 06:07:04] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
INFO [2020-04-15 06:07:04] Re-centering provided segment means (offset -0.0033).
INFO [2020-04-15 06:07:04] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:07:04] Setting undo.SD parameter to 0.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:07:04] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:07:04] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:07:04] Using 125 variants.
INFO [2020-04-15 06:07:04] Mean standard deviation of log-ratios: 0.40
INFO [2020-04-15 06:07:04] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:07:06] Local optima: 0.6/1.9, 0.4/2.1
INFO [2020-04-15 06:07:06] Testing local optimum 1/2 at purity 0.60 and total ploidy 1.90...
INFO [2020-04-15 06:07:07] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:07:07] Recalibrating log-ratios...
INFO [2020-04-15 06:07:07] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:07:07] Recalibrating log-ratios...
INFO [2020-04-15 06:07:07] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:07:08] Recalibrating log-ratios...
INFO [2020-04-15 06:07:08] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:07:08] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:07:08] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:07:08] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:07:09] Optimized purity: 0.65
INFO [2020-04-15 06:07:09] Done.
INFO [2020-04-15 06:07:09] ------------------------------------------------------------
WARN [2020-04-15 06:07:10] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:10] Allosome coverage missing, cannot determine sex.
Setting multi-figure configuration
FATAL [2020-04-15 06:07:20] Segmentation file expected with colnames ID, chrom, loc.start, loc.end, 

FATAL [2020-04-15 06:07:20] num.mark, seg.mean 

FATAL [2020-04-15 06:07:20]  

FATAL [2020-04-15 06:07:20] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:07:20] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:07:20] seg.file contains multiple samples and sampleid missing. 

FATAL [2020-04-15 06:07:20]  

FATAL [2020-04-15 06:07:20] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:07:20] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:07:21] seg.file contains multiple samples and sampleid does not match any. 

FATAL [2020-04-15 06:07:21]  

FATAL [2020-04-15 06:07:21] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:07:21] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:07:21] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:21] Allosome coverage missing, cannot determine sex.
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could noWARN [2020-04-15 06:07:30] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:30] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:30] Provided sampleid (example_tumor.txt) does not match Sample1 found in segmentation.
WARN [2020-04-15 06:07:35] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:35] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:37] No normalDB provided. Provide one for better results.
t parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line:WARN [2020-04-15 06:07:45] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:45] Allosome coverage missing, cannot determine sex.
 ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assWARN [2020-04-15 06:07:48] Provided sampleid (Sample.1) does not match Sample1 found in segmentation.
Setting multi-figure configuration
embly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19WARN [2020-04-15 06:07:57] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:58] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:07:58] No normalDB provided. Provide one for better results.
WARN [2020-04-15 06:07:58] Sampleid looks like a normal in VCF, not like a tumor.
WARN [2020-04-15 06:08:08] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:08] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:08:08] normalDB not a valid normalDB object. Use createNormalDatabase to 

FATAL [2020-04-15 06:08:08] create one. 

FATAL [2020-04-15 06:08:08]  

FATAL [2020-04-15 06:08:08] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:08:08] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:08:09] You are likely not using the correct baits file!
WARN [2020-04-15 06:08:09] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:09] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:11] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:11] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:08:11] normalDB appears to be empty. 

FATAL [2020-04-15 06:08:11]  

FATAL [2020-04-15 06:08:11] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:08:11] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:08:12] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:12] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:08:13] Intervals in coverage and interval.file have conflicting on/off-target annotation.
"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_linWARN [2020-04-15 06:08:48] normal.panel.vcf.file is deprecated, use mapping.bias.file instead.
e] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "#FATAL [2020-04-15 06:09:03] The normal.panel.vcf.file contains only a single sample. 

FATAL [2020-04-15 06:09:03]  

FATAL [2020-04-15 06:09:03] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:03] parameters (PureCN 1.16.0). 

== testthat results  ===========================================================
[ OK: 334 | SKIPPED: 0 | WARNINGS: 10 | FAILED: 0 ]
> 
> proc.time()
   user  system elapsed 
 464.04    4.53  698.35 
#contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">

PureCN.Rcheck/tests_x64/testthat.Rout


R version 3.6.3 (2020-02-29) -- "Holding the Windsock"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(PureCN)
Loading required package: DNAcopy
Loading required package: VariantAnnotation
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which, which.max, which.min

Loading required package: GenomeInfoDb
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:base':

    expand.grid

Loading required package: IRanges

Attaching package: 'IRanges'

The following object is masked from 'package:grDevices':

    windows

Loading required package: GenomicRanges
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: 'matrixStats'

The following objects are masked from 'package:Biobase':

    anyMissing, rowMedians

Loading required package: BiocParallel

Attaching package: 'DelayedArray'

The following objects are masked from 'package:matrixStats':

    colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges

The following objects are masked from 'package:base':

    aperm, apply, rowsum

Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit


Attaching package: 'VariantAnnotation'

The following object is masked from 'package:base':

    tabulate

> 
> test_check("PureCN")
WARN [2020-04-15 06:09:23] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
WARN [2020-04-15 06:09:23] Chromosome naming style of txdb file (UCSC) was different from interval file (NCBI).
WARN [2020-04-15 06:09:26] Attempted adding gene symbols to intervals. Heuristics have been used to pick symbols for overlapping genes.
FATAL [2020-04-15 06:09:29] tumor.coverage.file and interval.file do not align. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] Need either f or purity and ploidy. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] f not in expected range. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] coverage not in expected range (>=2) 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] purity not in expected range. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] ploidy not in expected range. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:09:29] cell.fraction not in expected range. 

FATAL [2020-04-15 06:09:29]  

FATAL [2020-04-15 06:09:29] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:09:29] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:09:32] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:09:32] You are likely not using the correct baits file!
WARN [2020-04-15 06:09:32] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:09:32] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:09:32] Processing on-target regions...
INFO [2020-04-15 06:09:32] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:09:32] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:09:35] Tumor/normal noise ratio: 19.793
WARN [2020-04-15 06:09:35] Extensive noise in tumor compared to normals.
FATAL [2020-04-15 06:10:30] pvalue.cutoff not within expected range or format. 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:30] pvalue.cutoff not within expected range or format. 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:30] percentile.cutoff not in expected range (0 to 100). 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:30] percentile.cutoff not in expected range (0 to 100). 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:30] purity not within expected range or format. 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:30] purity not within expected range or format. 

FATAL [2020-04-15 06:10:30]  

FATAL [2020-04-15 06:10:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:30] parameters (PureCN 1.16.0). 

[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] CouldINFO [2020-04-15 06:10:33] ------------------------------------------------------------
INFO [2020-04-15 06:10:33] PureCN 1.16.0
INFO [2020-04-15 06:10:33] ------------------------------------------------------------
INFO [2020-04-15 06:10:33] Loading coverage files...
INFO [2020-04-15 06:10:33] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:10:33] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:10:33] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:10:33] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:10:33] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:10:33] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:10:33] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:10:33] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:10:33] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:10:33] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:10:33] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:10:33] Loading VCF...
INFO [2020-04-15 06:10:33] Found 127 variants in VCF file.
INFO [2020-04-15 06:10:33] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:10:33] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:10:34] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:10:34] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:10:34] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:10:34] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:10:35] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:10:35] 1.0% of targets contain variants.
INFO [2020-04-15 06:10:35] Removing 2 variants outside intervals.
INFO [2020-04-15 06:10:35] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:10:35] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:10:35] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:10:35] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:10:35] Sample sex: ?
INFO [2020-04-15 06:10:35] Segmenting data...
INFO [2020-04-15 06:10:35] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:10:35] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:10:36] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:10:36] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:10:36] Using 123 variants.
INFO [2020-04-15 06:10:36] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:10:36] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:10:38] Local optima: 0.63/1.9, 0.4/2.2, 0.5/2
INFO [2020-04-15 06:10:38] Testing local optimum 1/3 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 06:10:40] Testing local optimum 2/3 at purity 0.40 and total ploidy 2.20...
INFO [2020-04-15 06:10:41] Testing local optimum 3/3 at purity 0.50 and total ploidy 2.00...
INFO [2020-04-15 06:10:42] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:10:42] Fitting variants for local optimum 1/3...
INFO [2020-04-15 06:10:43] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:10:44] Optimized purity: 0.65
INFO [2020-04-15 06:10:44] Fitting variants for local optimum 2/3...
INFO [2020-04-15 06:10:44] Fitting variants for purity 0.40, tumor ploidy 2.34 and contamination 0.01.
INFO [2020-04-15 06:10:45] Optimized purity: 0.40
INFO [2020-04-15 06:10:45] Done.
INFO [2020-04-15 06:10:45] ------------------------------------------------------------
FATAL [2020-04-15 06:10:48] exclude not a GRanges object. 

FATAL [2020-04-15 06:10:48]  

FATAL [2020-04-15 06:10:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:48] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:10:48] callable not a GRanges object. 

FATAL [2020-04-15 06:10:48]  

FATAL [2020-04-15 06:10:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:10:48] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:10:59] tumor.coverage.file and interval.file do not align.
FATAL [2020-04-15 06:11:02] No gc_bias column in interval.file. 

FATAL [2020-04-15 06:11:02]  

FATAL [2020-04-15 06:11:02] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:02] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:11:02] Provided coverage is zero, most likely due to a corrupt BAM file. 

FATAL [2020-04-15 06:11:02]  

FATAL [2020-04-15 06:11:02] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:02] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:11:08] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:08] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:08] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:08] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:09] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:09] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
FATAL [2020-04-15 06:11:09] Purity or Ploidy not numeric or in expected range. 

FATAL [2020-04-15 06:11:09]  

FATAL [2020-04-15 06:11:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:09] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:11:09] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
INFO [2020-04-15 06:11:09] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.rds...
FATAL [2020-04-15 06:11:09] 'Failed' column in 

FATAL [2020-04-15 06:11:09] C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f4326b2fa9.csv 

FATAL [2020-04-15 06:11:09] not logical(1). 

FATAL [2020-04-15 06:11:09]  

FATAL [2020-04-15 06:11:09] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:09] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:11:10] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:11:10] You are likely not using the correct baits file!
WARN [2020-04-15 06:11:10] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:10] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:10] Processing on-target regions...
INFO [2020-04-15 06:11:10] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:11:10] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:11:14] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:11:14] You are likely not using the correct baits file!
WARN [2020-04-15 06:11:14] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:14] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:14] Processing on-target regions...
INFO [2020-04-15 06:11:15] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:11:15] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:11:18] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:11:18] You are likely not using the correct baits file!
WARN [2020-04-15 06:11:18] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:18] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:18] Processing on-target regions...
INFO [2020-04-15 06:11:19] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:11:19] Removing 1 intervals with zero coverage in more than 3% of normalDB.
WARN [2020-04-15 06:11:20] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:20] Sample sex: NA
WARN [2020-04-15 06:11:21] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:21] Sample sex: NA
INFO [2020-04-15 06:11:22] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:11:22] You are likely not using the correct baits file!
WARN [2020-04-15 06:11:22] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:22] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:11:22] Length of normal.coverage.files and sex different 

FATAL [2020-04-15 06:11:22]  

FATAL [2020-04-15 06:11:22] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:22] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:11:28] Target intervals were not sorted.
INFO [2020-04-15 06:11:28] 560 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:11:28] You are likely not using the correct baits file!
WARN [2020-04-15 06:11:28] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:28] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:28] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:28] Processing on-target regions...
INFO [2020-04-15 06:11:28] Removing 978 intervals with low coverage in normalDB.
INFO [2020-04-15 06:11:28] Removing 11 intervals with zero coverage in more than 3% of normalDB.
FATAL [2020-04-15 06:11:33] tumor.coverage.file and normalDB do not align. 

FATAL [2020-04-15 06:11:33]  

FATAL [2020-04-15 06:11:33] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:33] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:11:33] At least 2 normal.coverage.files required. 

FATAL [2020-04-15 06:11:33]  

FATAL [2020-04-15 06:11:33] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:33] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:11:37] ------------------------------------------------------------
INFO [2020-04-15 06:11:37] PureCN 1.16.0
INFO [2020-04-15 06:11:37] ------------------------------------------------------------
INFO [2020-04-15 06:11:37] Loading coverage files...
INFO [2020-04-15 06:11:38] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:11:38] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:38] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:38] Removing 228 intervals with missing log.ratio.
FATAL [2020-04-15 06:11:38] normalDB incompatible with this PureCN version. Please re-run 

FATAL [2020-04-15 06:11:38] NormalDB.R. 

FATAL [2020-04-15 06:11:38]  

FATAL [2020-04-15 06:11:38] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:38] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:11:38] normalDB incompatible with this PureCN version. Please re-run 

FATAL [2020-04-15 06:11:38] NormalDB.R. 

FATAL [2020-04-15 06:11:38]  

FATAL [2020-04-15 06:11:38] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:38] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:11:38] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:39] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:39] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:39] Removing 16 low quality variants with BQ < 25.
INFO [2020-04-15 06:11:40] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:40] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:41] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:41] Removing 6 blacklisted variants.
INFO [2020-04-15 06:11:41] Removing 16 low quality variants with BQ < 25.
INFO [2020-04-15 06:11:41] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:42] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:42] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:42] Removing 16 low quality variants with BQ < 25.
WARN [2020-04-15 06:11:42] MuTect stats file lacks contig and position columns.
INFO [2020-04-15 06:11:42] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:43] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:43] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:43] Removing 16 low quality variants with BQ < 25.
WARN [2020-04-15 06:11:43] MuTect stats file and VCF file do not align perfectly. Will remove 2325 unmatched variants.
WARN [2020-04-15 06:11:43] MuTect stats file lacks failure_reasons column. Keeping all variants listed in stats file.
INFO [2020-04-15 06:11:43] Removing 0 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:44] Removing 0 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:44] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:11:44] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:45] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:45] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:45] Removing 22 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:11:46] Initial testing for significant sample cross-contamination: unlikely
INFO [2020-04-15 06:11:46] Removing 48 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:11:46] Found 11 variants in VCF file.
WARN [2020-04-15 06:11:47] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2020-04-15 06:11:47] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:11:51] Found 11 variants in VCF file.
WARN [2020-04-15 06:11:52] vcf.file has no DB info field for membership in germline databases. Found and used valid population allele frequency > 0.001000 instead.
INFO [2020-04-15 06:11:52] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:11:53] Found 11 variants in VCF file.
WARN [2020-04-15 06:11:53] vcf.file has no DB or POP_AF info field for membership in germline databases. Guessing it based on available dbSNP ID.
INFO [2020-04-15 06:11:53] 1 (9.1%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:11:54] Found 1000 variants in VCF file.
INFO [2020-04-15 06:11:54] Removing 2 triallelic sites.
WARN [2020-04-15 06:11:54] DP FORMAT field contains NAs. Removing 44 variants.
INFO [2020-04-15 06:11:54] 954 (100.0%) variants annotated as likely germline (DB INFO flag).
WARN [2020-04-15 06:11:56] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:11:56] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:11:56] Mean coverages: chrX: 108.58, chrY: 108.58, chr1-22: 104.87.
INFO [2020-04-15 06:11:57] Mean coverages: chrX: 108.58, chrY: 2.17, chr1-22: 104.87.
INFO [2020-04-15 06:11:57] Mean coverages: chrX: 108.58, chrY: 5.17, chr1-22: 104.87.
 not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the headINFO [2020-04-15 06:11:57] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:11:57] No germline variants in VCF.
FATAL [2020-04-15 06:11:58] No solution with id hello 

FATAL [2020-04-15 06:11:58]  

FATAL [2020-04-15 06:11:58] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:58] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:11:58] No solution with id 100 

FATAL [2020-04-15 06:11:58]  

FATAL [2020-04-15 06:11:58] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:58] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:11:59] all.data and w have different lengths. 

FATAL [2020-04-15 06:11:59]  

FATAL [2020-04-15 06:11:59] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:11:59] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:12:00] No mappability scores provided.
WARN [2020-04-15 06:12:00] No reptiming scores provided.
INFO [2020-04-15 06:12:00] Calculating GC-content...
WARN [2020-04-15 06:12:01] No mappability scores provided.
WARN [2020-04-15 06:12:01] No reptiming scores provided.
INFO [2020-04-15 06:12:01] Calculating GC-content...
INFO [2020-04-15 06:12:01] Removing 1 targets overlapping with exclude.
WARN [2020-04-15 06:12:01] No mappability scores provided.
WARN [2020-04-15 06:12:01] No reptiming scores provided.
INFO [2020-04-15 06:12:01] Calculating GC-content...
WARN [2020-04-15 06:12:01] Found small target regions (< 100bp). Will resize them.
FATAL [2020-04-15 06:12:01] off.target.padding must be negative. 

FATAL [2020-04-15 06:12:01]  

FATAL [2020-04-15 06:12:01] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:12:01] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:12:02] Interval coordinates should start at 1, not at 0 

FATAL [2020-04-15 06:12:02]  

FATAL [2020-04-15 06:12:02] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:12:02] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:12:02] Found small target regions (< 100bp). Will resize them.
FATAL [2020-04-15 06:12:02] No off-target regions after filtering for mappability and 

FATAL [2020-04-15 06:12:02] off.target.padding 

FATAL [2020-04-15 06:12:02]  

FATAL [2020-04-15 06:12:02] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:12:02] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:12:02] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:12:02] No mappability scores provided.
INFO [2020-04-15 06:12:02] Calculating GC-content...
WARN [2020-04-15 06:12:03] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:12:03] Averaging reptiming into bins of size 200...
WARN [2020-04-15 06:12:03] No mappability scores provided.
INFO [2020-04-15 06:12:03] Calculating GC-content...
WARN [2020-04-15 06:12:03] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:12:03] Splitting 1 large targets to an average width of 200.
WARN [2020-04-15 06:12:03] No mappability scores provided.
WARN [2020-04-15 06:12:03] No reptiming scores provided.
INFO [2020-04-15 06:12:03] Calculating GC-content...
WARN [2020-04-15 06:12:03] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:12:03] No mappability scores provided.
WARN [2020-04-15 06:12:03] No reptiming scores provided.
INFO [2020-04-15 06:12:03] Calculating GC-content...
INFO [2020-04-15 06:12:04] Tiling off-target regions to an average width of 200000.
WARN [2020-04-15 06:12:04] No mappability scores provided.
WARN [2020-04-15 06:12:04] No reptiming scores provided.
INFO [2020-04-15 06:12:04] Calculating GC-content...
WARN [2020-04-15 06:12:04] Intervals contain off-target regions. Will not change intervals.
WARN [2020-04-15 06:12:04] No mappability scores provided.
WARN [2020-04-15 06:12:04] No reptiming scores provided.
INFO [2020-04-15 06:12:04] Calculating GC-content...
WARN [2020-04-15 06:12:04] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:12:05] No reptiming scores provided.
INFO [2020-04-15 06:12:05] Calculating GC-content...
WARN [2020-04-15 06:12:06] Found small target regions (< 100bp). Will resize them.
WARN [2020-04-15 06:12:06] 1 intervals without mappability score (1 on-target).
INFO [2020-04-15 06:12:06] Removing 1 intervals with low mappability score (<0.50).
WARN [2020-04-15 06:12:06] No reptiming scores provided.
INFO [2020-04-15 06:12:06] Calculating GC-content...
WARN [2020-04-15 06:12:06] Found small target regions (< 100bp). Will resize them.
INFO [2020-04-15 06:12:06] Tiling off-target regions to an average width of 200000.
WARN [2020-04-15 06:12:07] No reptiming scores provided.
INFO [2020-04-15 06:12:07] Calculating GC-content...
WARN [2020-04-15 06:12:08] No mappability scores provided.
WARN [2020-04-15 06:12:08] No reptiming scores provided.
INFO [2020-04-15 06:12:08] Calculating GC-content...
WARN [2020-04-15 06:12:08] Chromosome naming style of interval file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:12:08] No mappability scores provided.
WARN [2020-04-15 06:12:08] No reptiming scores provided.
INFO [2020-04-15 06:12:08] Calculating GC-content...
FATAL [2020-04-15 06:12:08] Chromosome naming style of interval file unknown, should be UCSC. 

FATAL [2020-04-15 06:12:08]  

FATAL [2020-04-15 06:12:08] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:12:08] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:12:08] Chromosome naming style of interval file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:12:08] Chromosome naming style of mappability file (NCBI) was different from reference (UCSC).
WARN [2020-04-15 06:12:08] No reptiming scores provided.
INFO [2020-04-15 06:12:08] Calculating GC-content...
WARN [2020-04-15 06:12:09] Found small target regions (< 60bp). Will resize them.
WARN [2020-04-15 06:12:09] No mappability scores provided.
WARN [2020-04-15 06:12:09] No reptiming scores provided.
INFO [2020-04-15 06:12:09] Calculating GC-content...
WARN [2020-04-15 06:12:09] Found small target regions (< 60bp). Will drop them.
WARN [2020-04-15 06:12:09] No mappability scores provided.
WARN [2020-04-15 06:12:09] No reptiming scores provided.
INFO [2020-04-15 06:12:09] Calculating GC-content...
WARN [2020-04-15 06:12:09] Found small target regions (< 200bp). Will resize them.
WARN [2020-04-15 06:12:09] No mappability scores provided.
WARN [2020-04-15 06:12:09] No reptiming scores provided.
INFO [2020-04-15 06:12:09] Calculating GC-content...
INFO [2020-04-15 06:12:10] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:12:10] You are likely not using the correct baits file!
WARN [2020-04-15 06:12:10] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:12:10] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:12:10] Processing on-target regions...
INFO [2020-04-15 06:12:11] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:12:11] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:12:15] Interval weights found, but currently not supported by copynumber. Will simply exclude intervals with low weight.
multipcf finished for chromosome arm 1p 
multipcf finished for chromosome arm 1q 
multipcf finished for chromosome arm 2p 
multipcf finished for chromosome arm 2q 
multipcf finished for chromosome arm 3p 
multipcf finished for chromosome arm 3q 
multipcf finished for chromosome arm 4p 
multipcf finished for chromosome arm 4q 
multipcf finished for chromosome arm 5p 
multipcf finished for chromosome arm 5q 
multipcf finished for chromosome arm 6p 
multipcf finished for chromosome arm 6q 
multipcf finished for chromosome arm 7p 
multipcf finished for chromosome arm 7q 
multipcf finished for chromosome arm 8p 
multipcf finished for chromosome arm 8q 
multipcf finished for chromosome arm 9p 
multipcf finished for chromosome arm 9q 
multipcf finished for chromosome arm 10p 
multipcf finished for chromosome arm 10q 
multipcf finished for chromosome arm 11p 
multipcf finished for chromosome arm 11q 
multipcf finished for chromosome arm 12p 
multipcf finished for chromosome arm 12q 
multipcf finished for chromosome arm 13q 
multipcf finished for chromosome arm 14q 
multipcf finished for chromosome arm 15q 
multipcf finished for chromosome arm 16p 
multipcf finished for chromosome arm 16q 
multipcf finished for chromosome arm 17p 
multipcf finished for chromosome arm 17q 
multipcf finished for chromosome arm 18p 
multipcf finished for chromosome arm 18q 
multipcf finished for chromosome arm 19p 
multipcf finished for chromosome arm 19q 
multipcf finished for chromosome arm 20p 
multipcf finished for chromosome arm 20q 
multipcf finished for chromosome arm 21q 
multipcf finished for chromosome arm 22q 
INFO [2020-04-15 06:12:17] ------------------------------------------------------------
INFO [2020-04-15 06:12:17] PureCN 1.16.0
INFO [2020-04-15 06:12:17] ------------------------------------------------------------
INFO [2020-04-15 06:12:17] Loading coverage files...
INFO [2020-04-15 06:12:17] seg.file and normal.coverage.file provided. Using both.
INFO [2020-04-15 06:12:17] Mean target coverages: 112X (tumor) 105X (normal).
WARN [2020-04-15 06:12:17] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:12:17] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:12:17] Removing 199 intervals with missing log.ratio.
INFO [2020-04-15 06:12:17] Using 9850 intervals (9850 on-target, 0 off-target).
INFO [2020-04-15 06:12:17] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:12:17] Loading VCF...
er line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2INFO [2020-04-15 06:12:18] Found 127 variants in VCF file.
INFO [2020-04-15 06:12:18] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:12:18] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:12:18] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:12:18] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:12:19] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:12:19] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:12:19] Total size of targeted genomic region: 1.68Mb (2.66Mb with 50bp padding).
INFO [2020-04-15 06:12:19] 1.0% of targets contain variants.
INFO [2020-04-15 06:12:19] Removing 0 variants outside intervals.
INFO [2020-04-15 06:12:19] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:12:19] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:12:19] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2020-04-15 06:12:19] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:12:19] Sample sex: ?
INFO [2020-04-15 06:12:19] Segmenting data...
INFO [2020-04-15 06:12:19] Loaded provided segmentation file file5f476a64e5d.seg (format DNAcopy).
INFO [2020-04-15 06:12:19] Re-centering provided segment means (offset 0.0110).
INFO [2020-04-15 06:12:19] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:12:19] Found 59 segments with median size of 30.15Mb.
INFO [2020-04-15 06:12:20] Using 125 variants.
INFO [2020-04-15 06:12:20] Mean standard deviation of log-ratios: 0.34
INFO [2020-04-15 06:12:20] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:12:21] Local optima: 0.6/1.9, 0.4/2.1
INFO [2020-04-15 06:12:21] Testing local optimum 1/2 at purity 0.60 and total ploidy 1.90...
INFO [2020-04-15 06:12:22] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:12:22] Recalibrating log-ratios...
INFO [2020-04-15 06:12:22] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:12:23] Recalibrating log-ratios...
INFO [2020-04-15 06:12:23] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:12:23] Recalibrating log-ratios...
INFO [2020-04-15 06:12:23] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:12:23] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:12:23] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:12:23] Fitting variants for purity 0.60, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:25] Fitting variants for purity 0.40, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:26] Fitting variants for purity 0.45, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:27] Fitting variants for purity 0.50, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:29] Fitting variants for purity 0.55, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:31] Fitting variants for purity 0.65, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:32] Fitting variants for purity 0.70, tumor ploidy 1.76 and contamination 0.01.
INFO [2020-04-15 06:12:34] Optimized purity: 0.65
INFO [2020-04-15 06:12:34] Done.
INFO [2020-04-15 06:12:34] ------------------------------------------------------------
INFO [2020-04-15 06:12:36] Loaded provided segmentation file example_seg.txt (format DNAcopy).
INFO [2020-04-15 06:12:37] Re-centering provided segment means (offset -0.0033).
INFO [2020-04-15 06:12:37] Loaded provided segmentation file example_gatk4_modelfinal.seg.gz (format GATK4).
WARN [2020-04-15 06:12:37] Expecting numeric chromosome names in seg.file, assuming file is properly sorted.
INFO [2020-04-15 06:12:37] Re-centering provided segment means (offset -0.0037).
INFO [2020-04-15 06:12:37] 576 on-target bins with low coverage in all samples.
WARN [2020-04-15 06:12:37] You are likely not using the correct baits file!
WARN [2020-04-15 06:12:37] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:12:37] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:12:38] Processing on-target regions...
INFO [2020-04-15 06:12:38] Removing 930 intervals with low coverage in normalDB.
INFO [2020-04-15 06:12:38] Removing 1 intervals with zero coverage in more than 3% of normalDB.
INFO [2020-04-15 06:12:40] ------------------------------------------------------------
INFO [2020-04-15 06:12:40] PureCN 1.16.0
INFO [2020-04-15 06:12:40] ------------------------------------------------------------
INFO [2020-04-15 06:12:41] Using BiocParallel for parallel optimization.
INFO [2020-04-15 06:12:41] Loading coverage files...
INFO [2020-04-15 06:12:42] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:12:42] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:12:42] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:12:42] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:12:42] Removing 705 intervals excluded in normalDB.
INFO [2020-04-15 06:12:42] normalDB provided. Setting minimum coverage for segmentation to 0.0015X.
INFO [2020-04-15 06:12:42] Using 9116 intervals (9116 on-target, 0 off-target).
INFO [2020-04-15 06:12:42] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:12:42] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:12:42] Sample sex: ?
INFO [2020-04-15 06:12:42] Segmenting data...
INFO [2020-04-15 06:12:43] Interval weights found, will use weighted CBS.
INFO [2020-04-15 06:12:43] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:12:43] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:12:45] Found 48 segments with median size of 31.71Mb.
INFO [2020-04-15 06:12:45] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:12:45] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:12:45] Local optima: 0.62/1.9, 0.38/2.2, 0.31/1.9, 0.27/2, 0.78/2
INFO [2020-04-15 06:13:47] Skipping 2 solutions that converged to the same optima.
INFO [2020-04-15 06:13:47] Skipping 2 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:13:47] Done.
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] Reading C:\Users\biocbuild\bbs-3.10-bioc\tmpdir\RtmpK8QKMn\file5f453294b35.rds...
FATAL [2020-04-15 06:13:47] runAbsoluteCN was run without a VCF file. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:13:47] runAbsoluteCN was run without a VCF file. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] Loading coverage files...
FATAL [2020-04-15 06:13:47] Need a normal coverage file if log.ratio and seg.file are not provided. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
FATAL [2020-04-15 06:13:47] min.ploidy or max.ploidy not within expected range. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
FATAL [2020-04-15 06:13:47] min.ploidy or max.ploidy not within expected range. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
FATAL [2020-04-15 06:13:47] test.num.copy not within expected range. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
WARN [2020-04-15 06:13:47] test.num.copy outside recommended range.
FATAL [2020-04-15 06:13:47] max.non.clonal not within expected range or format. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
WARN [2020-04-15 06:13:47] test.num.copy outside recommended range.
FATAL [2020-04-15 06:13:47] max.non.clonal not within expected range or format. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
FATAL [2020-04-15 06:13:47] test.purity not within expected range. 

FATAL [2020-04-15 06:13:47]  

FATAL [2020-04-15 06:13:47] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:47] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] PureCN 1.16.0
INFO [2020-04-15 06:13:47] ------------------------------------------------------------
INFO [2020-04-15 06:13:47] Loading coverage files...
FATAL [2020-04-15 06:13:48] Tumor and normal are identical. This won't give any meaningful results 

FATAL [2020-04-15 06:13:48] and I'm stopping here. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] Loading coverage files...
FATAL [2020-04-15 06:13:48] Length of log.ratio different from tumor coverage. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] prior.purity must have the same length as test.purity. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] min.gof not within expected range or format. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] prior.purity not within expected range or format. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] prior.purity must add to 1. Sum is 1.5 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] max.homozygous.loss not within expected range or format. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] prior.K not within expected range or format. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] prior.contamination not within expected range or format. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] Iterations not in the expected range from 10 to 250. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
FATAL [2020-04-15 06:13:48] Iterations not in the expected range from 10 to 250. 

FATAL [2020-04-15 06:13:48]  

FATAL [2020-04-15 06:13:48] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:48] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] PureCN 1.16.0
INFO [2020-04-15 06:13:48] ------------------------------------------------------------
INFO [2020-04-15 06:13:48] Loading coverage files...
FATAL [2020-04-15 06:13:49] Missing tumor.coverage.file requires seg.file or log.ratio and 

FATAL [2020-04-15 06:13:49] interval.file. 

FATAL [2020-04-15 06:13:49]  

FATAL [2020-04-15 06:13:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:49] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] PureCN 1.16.0
INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] PureCN 1.16.0
INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] Loading coverage files...
FATAL [2020-04-15 06:13:49] Interval files in normal and tumor different. 

FATAL [2020-04-15 06:13:49]  

FATAL [2020-04-15 06:13:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:49] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] PureCN 1.16.0
INFO [2020-04-15 06:13:49] ------------------------------------------------------------
INFO [2020-04-15 06:13:49] Loading coverage files...
INFO [2020-04-15 06:13:50] Mean target coverages: 0X (tumor) 99X (normal).
WARN [2020-04-15 06:13:50] Large difference in coverage of tumor and normal.
FATAL [2020-04-15 06:13:50] No finite intervals. 

FATAL [2020-04-15 06:13:50]  

FATAL [2020-04-15 06:13:50] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:50] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:50] ------------------------------------------------------------
INFO [2020-04-15 06:13:50] PureCN 1.16.0
INFO [2020-04-15 06:13:50] ------------------------------------------------------------
INFO [2020-04-15 06:13:50] Loading coverage files...
INFO [2020-04-15 06:13:50] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:13:50] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:13:50] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:13:50] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:13:50] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:13:50] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:13:50] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:13:50] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:13:50] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:13:50] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:13:50] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:13:50] Loading VCF...
2,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""INFO [2020-04-15 06:13:51] Found 127 variants in VCF file.
INFO [2020-04-15 06:13:51] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:13:51] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:13:51] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:13:51] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:13:52] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
Error in FileForFormat(con) : Format 'txt' unsupported
FATAL [2020-04-15 06:13:52] Could not import snp.blacklist 

FATAL [2020-04-15 06:13:52] C:/Users/biocbuild/bbs-3.10-bioc/R/library/PureCN/extdata/example_normal.txt:Error 

FATAL [2020-04-15 06:13:52] in FileForFormat(con) : Format 'txt' unsupported 

FATAL [2020-04-15 06:13:52]  

FATAL [2020-04-15 06:13:52] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:13:52] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:13:52] ------------------------------------------------------------
INFO [2020-04-15 06:13:52] PureCN 1.16.0
INFO [2020-04-15 06:13:52] ------------------------------------------------------------
INFO [2020-04-15 06:13:52] Loading coverage files...
INFO [2020-04-15 06:13:52] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:13:52] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:13:52] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:13:52] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:13:52] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:13:52] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:13:52] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:13:52] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:13:52] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:13:52] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:13:52] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:13:52] Loading VCF...
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_INFO [2020-04-15 06:13:53] Found 127 variants in VCF file.
INFO [2020-04-15 06:13:53] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:13:53] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:13:53] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:13:53] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:13:54] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:13:54] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:13:54] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:13:54] 1.0% of targets contain variants.
INFO [2020-04-15 06:13:54] Removing 2 variants outside intervals.
INFO [2020-04-15 06:13:54] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:13:54] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:13:54] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:13:54] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:13:54] Sample sex: ?
INFO [2020-04-15 06:13:54] Segmenting data...
INFO [2020-04-15 06:13:54] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:13:54] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:13:56] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:13:56] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:13:56] Using 123 variants.
INFO [2020-04-15 06:13:56] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:13:56] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:13:57] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2020-04-15 06:13:57] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:13:58] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:13:59] Recalibrating log-ratios...
INFO [2020-04-15 06:13:59] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:13:59] Recalibrating log-ratios...
INFO [2020-04-15 06:13:59] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:00] Recalibrating log-ratios...
INFO [2020-04-15 06:14:00] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:00] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:00] Recalibrating log-ratios...
INFO [2020-04-15 06:14:00] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:02] Recalibrating log-ratios...
INFO [2020-04-15 06:14:02] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:02] Recalibrating log-ratios...
INFO [2020-04-15 06:14:02] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:02] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:14:02] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:14:02] Fitting variants for local optimum 1/3...
INFO [2020-04-15 06:14:02] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:14:04] Optimized purity: 0.65
INFO [2020-04-15 06:14:04] Done.
INFO [2020-04-15 06:14:04] ------------------------------------------------------------
parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parINFO [2020-04-15 06:14:04] ------------------------------------------------------------
INFO [2020-04-15 06:14:04] PureCN 1.16.0
INFO [2020-04-15 06:14:04] ------------------------------------------------------------
INFO [2020-04-15 06:14:04] Loading coverage files...
INFO [2020-04-15 06:14:05] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:14:05] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:14:05] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:14:05] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:14:05] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:14:05] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:14:05] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:14:05] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:14:05] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:14:05] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:14:05] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:14:05] Loading VCF...
INFO [2020-04-15 06:14:05] Found 127 variants in VCF file.
WARN [2020-04-15 06:14:05] DP FORMAT field contains NAs. Removing 3 variants.
WARN [2020-04-15 06:14:05] DB INFO flag contains NAs
INFO [2020-04-15 06:14:05] 119 (96.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:14:05] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:14:05] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:14:06] Removing 1 variants with AF < 0.030 or AF >= 0.970 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:14:06] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:14:06] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:14:06] 1.0% of targets contain variants.
INFO [2020-04-15 06:14:06] Setting somatic prior probabilities for dbSNP hits to 0.000500 or to 0.500000 otherwise.
INFO [2020-04-15 06:14:06] Excluding 5 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:14:06] Sample sex: ?
INFO [2020-04-15 06:14:06] Segmenting data...
INFO [2020-04-15 06:14:06] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:14:07] Setting undo.SD parameter to 1.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:14:08] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:14:08] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:14:08] Using 123 variants.
INFO [2020-04-15 06:14:08] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:14:08] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:14:10] Local optima: 0.62/1.9, 0.3/1.9, 0.32/2
INFO [2020-04-15 06:14:10] Testing local optimum 1/3 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:14:11] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:11] Recalibrating log-ratios...
INFO [2020-04-15 06:14:11] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:11] Recalibrating log-ratios...
INFO [2020-04-15 06:14:12] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:12] Recalibrating log-ratios...
INFO [2020-04-15 06:14:12] Testing local optimum 2/3 at purity 0.30 and total ploidy 1.90...
INFO [2020-04-15 06:14:12] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:13] Recalibrating log-ratios...
INFO [2020-04-15 06:14:13] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:13] Recalibrating log-ratios...
INFO [2020-04-15 06:14:13] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:13] Recalibrating log-ratios...
INFO [2020-04-15 06:14:13] Testing local optimum 3/3 at purity 0.32 and total ploidy 2.00...
INFO [2020-04-15 06:14:14] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:14:14] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:14:14] Fitting variants for local optimum 1/3...
INFO [2020-04-15 06:14:14] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:14:15] Optimized purity: 0.65
INFO [2020-04-15 06:14:15] Done.
INFO [2020-04-15 06:14:15] ------------------------------------------------------------
INFO [2020-04-15 06:14:15] ------------------------------------------------------------
INFO [2020-04-15 06:14:15] PureCN 1.16.0
INFO [2020-04-15 06:14:15] ------------------------------------------------------------
INFO [2020-04-15 06:14:15] Loading coverage files...
INFO [2020-04-15 06:14:16] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:14:16] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:14:16] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:14:16] No Gene column in interval.file. You won't get gene-level calls.
INFO [2020-04-15 06:14:16] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:14:16] Removing 15 low/high GC targets.
INFO [2020-04-15 06:14:16] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:14:16] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:14:16] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:14:16] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:14:16] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:14:16] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:14:16] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:14:16] Loading VCF...
se the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=INFO [2020-04-15 06:14:17] Found 127 variants in VCF file.
INFO [2020-04-15 06:14:17] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:14:17] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:14:17] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:14:17] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:14:18] Removing 1 variants with AF < 0.030 or AF >= Inf or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:14:18] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:14:18] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:14:18] 1.0% of targets contain variants.
INFO [2020-04-15 06:14:18] Removing 2 variants outside intervals.
INFO [2020-04-15 06:14:18] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:14:18] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:14:18] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:14:18] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:14:18] Sample sex: ?
INFO [2020-04-15 06:14:18] Segmenting data...
INFO [2020-04-15 06:14:19] Using unweighted PSCBS.
INFO [2020-04-15 06:14:19] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:14:38] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:14:38] Found 70 segments with median size of 28.20Mb.
INFO [2020-04-15 06:14:38] Using 123 variants.
INFO [2020-04-15 06:14:38] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:14:38] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:14:38] Local optima: 0.62/1.9, 0.38/2.2, 0.31/1.9, 0.27/2, 0.78/2
INFO [2020-04-15 06:14:38] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:14:39] Recalibrating log-ratios...
INFO [2020-04-15 06:14:39] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:14:39] Recalibrating log-ratios...
INFO [2020-04-15 06:14:39] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:14:39] Recalibrating log-ratios...
INFO [2020-04-15 06:14:39] Testing local optimum 1/5 at purity 0.62 and total ploidy 1.90...
INFO [2020-04-15 06:14:40] Testing local optimum 2/5 at purity 0.38 and total ploidy 2.20...
INFO [2020-04-15 06:14:42] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:14:43] Recalibrating log-ratios...
INFO [2020-04-15 06:14:43] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:14:43] Recalibrating log-ratios...
INFO [2020-04-15 06:14:43] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:14:43] Recalibrating log-ratios...
INFO [2020-04-15 06:14:43] Testing local optimum 3/5 at purity 0.31 and total ploidy 1.90...
INFO [2020-04-15 06:14:44] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:14:44] Recalibrating log-ratios...
INFO [2020-04-15 06:14:45] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:14:45] Recalibrating log-ratios...
INFO [2020-04-15 06:14:45] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:14:45] Recalibrating log-ratios...
INFO [2020-04-15 06:14:45] Testing local optimum 4/5 at purity 0.27 and total ploidy 2.00...
INFO [2020-04-15 06:14:46] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:14:46] Recalibrating log-ratios...
INFO [2020-04-15 06:14:46] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:14:47] Recalibrating log-ratios...
INFO [2020-04-15 06:14:47] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:14:47] Recalibrating log-ratios...
INFO [2020-04-15 06:14:47] Testing local optimum 5/5 at purity 0.78 and total ploidy 2.00...
INFO [2020-04-15 06:14:47] Skipping 1 solutions that converged to the same optima.
INFO [2020-04-15 06:14:47] Skipping 3 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:14:47] Fitting variants for local optimum 2/5...
INFO [2020-04-15 06:14:47] Fitting variants for purity 0.40, tumor ploidy 2.55 and contamination 0.01.
INFO [2020-04-15 06:14:49] Optimized purity: 0.40
INFO [2020-04-15 06:14:49] Done.
INFO [2020-04-15 06:14:49] ------------------------------------------------------------
FATAL [2020-04-15 06:14:49] This function requires gene-level calls. Please add a column 'Gene' 

FATAL [2020-04-15 06:14:49] containing gene symbols to the interval.file. 

FATAL [2020-04-15 06:14:49]  

FATAL [2020-04-15 06:14:49] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:14:49] parameters (PureCN 1.16.0). 

<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=INFO [2020-04-15 06:14:50] ------------------------------------------------------------
INFO [2020-04-15 06:14:50] PureCN 1.16.0
INFO [2020-04-15 06:14:50] ------------------------------------------------------------
INFO [2020-04-15 06:14:50] Loading coverage files...
INFO [2020-04-15 06:14:51] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:14:51] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:14:51] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:14:51] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:14:51] Removing 22 small (< 5bp) intervals.
INFO [2020-04-15 06:14:51] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:14:51] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:14:51] Removing 233 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:14:51] Using 9547 intervals (9547 on-target, 0 off-target).
INFO [2020-04-15 06:14:51] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:14:51] No interval.file provided. Cannot check if data was GC-normalized. Was it?
INFO [2020-04-15 06:14:51] Loading VCF...
INFO [2020-04-15 06:14:51] Found 127 variants in VCF file.
INFO [2020-04-15 06:14:51] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
FATAL [2020-04-15 06:14:51] Different chromosome names in coverage and VCF. 

FATAL [2020-04-15 06:14:51]  

FATAL [2020-04-15 06:14:51] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:14:51] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:14:51] ------------------------------------------------------------
INFO [2020-04-15 06:14:51] PureCN 1.16.0
INFO [2020-04-15 06:14:51] ------------------------------------------------------------
INFO [2020-04-15 06:14:51] Loading coverage files...
INFO [2020-04-15 06:14:51] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:14:51] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:14:51] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:14:51] tumor.coverage.file and interval.file do not align. 

FATAL [2020-04-15 06:14:51]  

FATAL [2020-04-15 06:14:51] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:14:51] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:14:52] ------------------------------------------------------------
INFO [2020-04-15 06:14:52] PureCN 1.16.0
INFO [2020-04-15 06:14:52] ------------------------------------------------------------
INFO [2020-04-15 06:14:52] Loading coverage files...
INFO [2020-04-15 06:14:52] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:14:52] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:14:52] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:14:53] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:14:53] Removing 15 low/high GC targets.
INFO [2020-04-15 06:14:53] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:14:53] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:14:53] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:14:53] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:14:53] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:14:53] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:14:53] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:14:53] Loading VCF...
INFO [2020-04-15 06:14:53] Found 127 variants in VCF file.
INFO [2020-04-15 06:14:53] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:14:54] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:14:54] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:14:54] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:14:54] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:14:54] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:14:55] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:14:55] 1.0% of targets contain variants.
INFO [2020-04-15 06:14:55] Removing 2 variants outside intervals.
INFO [2020-04-15 06:14:55] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:14:55] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:14:55] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:14:55] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:14:55] Sample sex: ?
INFO [2020-04-15 06:14:55] Segmenting data...
INFO [2020-04-15 06:14:55] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:14:55] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:14:56] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:14:56] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:14:56] Using 123 variants.
INFO [2020-04-15 06:14:56] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:14:56] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:14:57] Local optima: 0.63/1.9, 0.4/2.1
INFO [2020-04-15 06:14:57] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 06:14:58] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:14:59] Recalibrating log-ratios...
INFO [2020-04-15 06:14:59] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:14:59] Recalibrating log-ratios...
INFO [2020-04-15 06:14:59] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:00] Recalibrating log-ratios...
INFO [2020-04-15 06:15:00] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:00] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:15:00] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:15:00] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:15:01] Optimized purity: 0.65
INFO [2020-04-15 06:15:01] Done.
INFO [2020-04-15 06:15:01] ------------------------------------------------------------
INFO [2020-04-15 06:15:01] ------------------------------------------------------------
INFO [2020-04-15 06:15:01] PureCN 1.16.0
INFO [2020-04-15 06:15:01] ------------------------------------------------------------
INFO [2020-04-15 06:15:02] Loading coverage files...
INFO [2020-04-15 06:15:02] Mean target coverages: 112X (tumor) 99X (normal).
WARN [2020-04-15 06:15:02] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:02] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:15:03] Removing 228 intervals with missing log.ratio.
INFO [2020-04-15 06:15:03] Removing 15 low/high GC targets.
INFO [2020-04-15 06:15:03] Removing 21 small (< 5bp) intervals.
INFO [2020-04-15 06:15:03] Removing 19 intervals with low total coverage in normal (< 150.00 reads).
WARN [2020-04-15 06:15:03] No normalDB provided. Provide one for better results.
INFO [2020-04-15 06:15:03] Removing 232 low coverage (< 15.0000X) intervals.
INFO [2020-04-15 06:15:03] Using 9534 intervals (9534 on-target, 0 off-target).
INFO [2020-04-15 06:15:03] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:15:03] AT/GC dropout: 1.03 (tumor), 1.03 (normal). 
INFO [2020-04-15 06:15:03] Loading VCF...
INFO [2020-04-15 06:15:03] Found 127 variants in VCF file.
INFO [2020-04-15 06:15:03] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:15:03] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:15:03] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:15:04] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:15:04] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:15:04] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:15:04] Total size of targeted genomic region: 1.63Mb (2.58Mb with 50bp padding).
INFO [2020-04-15 06:15:04] 1.0% of targets contain variants.
INFO [2020-04-15 06:15:04] Removing 2 variants outside intervals.
INFO [2020-04-15 06:15:04] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:15:04] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:15:04] Found SOMATIC annotation in VCF. Setting mapping bias to 0.973.
INFO [2020-04-15 06:15:04] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:15:05] Sample sex: ?
INFO [2020-04-15 06:15:05] Segmenting data...
INFO [2020-04-15 06:15:05] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:15:05] Setting undo.SD parameter to 1.000000.
INFO [2020-04-15 06:15:06] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:15:06] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:15:06] Using 123 variants.
INFO [2020-04-15 06:15:06] Mean standard deviation of log-ratios: 0.37
INFO [2020-04-15 06:15:06] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:15:07] Local optima: 0.63/1.9, 0.4/2.1
INFO [2020-04-15 06:15:07] Testing local optimum 1/2 at purity 0.63 and total ploidy 1.90...
INFO [2020-04-15 06:15:08] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:09] Recalibrating log-ratios...
INFO [2020-04-15 06:15:09] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:09] Recalibrating log-ratios...
INFO [2020-04-15 06:15:09] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:09] Recalibrating log-ratios...
INFO [2020-04-15 06:15:09] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:10] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:15:10] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:15:10] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:15:12] Optimized purity: 0.65
INFO [2020-04-15 06:15:12] Done.
INFO [2020-04-15 06:15:12] ------------------------------------------------------------
FATAL [2020-04-15 06:15:12] chr1 not valid chromosome name(s). Valid names are: 

FATAL [2020-04-15 06:15:12] 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22 

FATAL [2020-04-15 06:15:12]  

FATAL [2020-04-15 06:15:12] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:15:12] parameters (PureCN 1.16.0). 

INFO [2020-04-15 06:15:12] ------------------------------------------------------------
INFO [2020-04-15 06:15:12] PureCN 1.16.0
INFO [2020-04-15 06:15:12] ------------------------------------------------------------
INFO [2020-04-15 06:15:12] Loading coverage files...
WARN [2020-04-15 06:15:12] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
WARN [2020-04-15 06:15:12] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:12] Allosome coverage missing, cannot determine sex.
INFO [2020-04-15 06:15:12] Removing 10 intervals with missing log.ratio.
INFO [2020-04-15 06:15:12] Using 10039 intervals (10039 on-target, 0 off-target).
INFO [2020-04-15 06:15:12] No off-target intervals. If this is hybrid-capture data, consider adding them.
INFO [2020-04-15 06:15:12] Loading VCF...
90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
INFO [2020-04-15 06:15:12] Found 127 variants in VCF file.
INFO [2020-04-15 06:15:12] 127 (100.0%) variants annotated as likely germline (DB INFO flag).
INFO [2020-04-15 06:15:13] LIB-02240e4 is tumor in VCF file.
INFO [2020-04-15 06:15:13] No homozygous variants in VCF, provide unfiltered VCF.
INFO [2020-04-15 06:15:13] Removing 1 non heterozygous (in matched normal) germline SNPs.
INFO [2020-04-15 06:15:14] Removing 1 variants with AF < 0.030 or AF >= 1.000 or less than 4 supporting reads or depth < 15.
INFO [2020-04-15 06:15:14] Removing 0 low quality variants with BQ < 25.
INFO [2020-04-15 06:15:14] Total size of targeted genomic region: 1.71Mb (2.70Mb with 50bp padding).
INFO [2020-04-15 06:15:14] 1.0% of targets contain variants.
INFO [2020-04-15 06:15:14] Removing 0 variants outside intervals.
INFO [2020-04-15 06:15:14] Found SOMATIC annotation in VCF.
INFO [2020-04-15 06:15:14] Setting somatic prior probabilities for somatic variants to 0.999000 or to 0.000100 otherwise.
INFO [2020-04-15 06:15:14] Found SOMATIC annotation in VCF. Setting mapping bias to 0.976.
INFO [2020-04-15 06:15:14] Excluding 0 novel or poor quality variants from segmentation.
INFO [2020-04-15 06:15:14] Sample sex: ?
INFO [2020-04-15 06:15:14] Segmenting data...
INFO [2020-04-15 06:15:14] Loaded provided segmentation file example_seg.txt (format DNAcopy).
WARN [2020-04-15 06:15:14] Provided sampleid (Sample2) does not match Sample1 found in segmentation.
INFO [2020-04-15 06:15:14] Re-centering provided segment means (offset -0.0033).
INFO [2020-04-15 06:15:14] Loading pre-computed boundaries for DNAcopy...
INFO [2020-04-15 06:15:14] Setting undo.SD parameter to 0.000000.
Setting multi-figure configuration
INFO [2020-04-15 06:15:15] Setting prune.hclust.h parameter to 0.200000.
INFO [2020-04-15 06:15:15] Found 54 segments with median size of 24.88Mb.
INFO [2020-04-15 06:15:15] Using 125 variants.
INFO [2020-04-15 06:15:15] Mean standard deviation of log-ratios: 0.40
INFO [2020-04-15 06:15:15] 2D-grid search of purity and ploidy...
INFO [2020-04-15 06:15:16] Local optima: 0.6/1.9, 0.4/2.1
INFO [2020-04-15 06:15:16] Testing local optimum 1/2 at purity 0.60 and total ploidy 1.90...
INFO [2020-04-15 06:15:17] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:17] Recalibrating log-ratios...
INFO [2020-04-15 06:15:17] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:18] Recalibrating log-ratios...
INFO [2020-04-15 06:15:18] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:18] Recalibrating log-ratios...
INFO [2020-04-15 06:15:18] Testing local optimum 2/2 at purity 0.40 and total ploidy 2.10...
INFO [2020-04-15 06:15:19] Skipping 1 solutions exceeding max.non.clonal (0.20).
INFO [2020-04-15 06:15:19] Fitting variants for local optimum 1/2...
INFO [2020-04-15 06:15:19] Fitting variants for purity 0.65, tumor ploidy 1.74 and contamination 0.01.
INFO [2020-04-15 06:15:21] Optimized purity: 0.65
INFO [2020-04-15 06:15:21] Done.
INFO [2020-04-15 06:15:21] ------------------------------------------------------------
WARN [2020-04-15 06:15:21] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:21] Allosome coverage missing, cannot determine sex.
Setting multi-figure configuration
FATAL [2020-04-15 06:15:31] Segmentation file expected with colnames ID, chrom, loc.start, loc.end, 

FATAL [2020-04-15 06:15:31] num.mark, seg.mean 

FATAL [2020-04-15 06:15:31]  

FATAL [2020-04-15 06:15:31] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:15:31] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:15:32] seg.file contains multiple samples and sampleid missing. 

FATAL [2020-04-15 06:15:32]  

FATAL [2020-04-15 06:15:32] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:15:32] parameters (PureCN 1.16.0). 

FATAL [2020-04-15 06:15:32] seg.file contains multiple samples and sampleid does not match any. 

FATAL [2020-04-15 06:15:32]  

FATAL [2020-04-15 06:15:32] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:15:32] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:15:34] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:34] Allosome coverage missing, cannot determine sex.
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could noWARN [2020-04-15 06:15:44] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:44] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:44] Provided sampleid (example_tumor.txt) does not match Sample1 found in segmentation.
WARN [2020-04-15 06:15:49] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:49] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:50] No normalDB provided. Provide one for better results.
t parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line:WARN [2020-04-15 06:15:59] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:15:59] Allosome coverage missing, cannot determine sex.
 ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assWARN [2020-04-15 06:16:02] Provided sampleid (Sample.1) does not match Sample1 found in segmentation.
Setting multi-figure configuration
embly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19WARN [2020-04-15 06:16:14] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:14] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:14] No normalDB provided. Provide one for better results.
WARN [2020-04-15 06:16:15] Sampleid looks like a normal in VCF, not like a tumor.
WARN [2020-04-15 06:16:26] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:26] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:16:26] normalDB not a valid normalDB object. Use createNormalDatabase to 

FATAL [2020-04-15 06:16:26] create one. 

FATAL [2020-04-15 06:16:26]  

FATAL [2020-04-15 06:16:26] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:16:26] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:16:26] You are likely not using the correct baits file!
WARN [2020-04-15 06:16:26] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:27] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:30] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:30] Allosome coverage missing, cannot determine sex.
FATAL [2020-04-15 06:16:30] normalDB appears to be empty. 

FATAL [2020-04-15 06:16:30]  

FATAL [2020-04-15 06:16:30] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:16:30] parameters (PureCN 1.16.0). 

WARN [2020-04-15 06:16:31] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:31] Allosome coverage missing, cannot determine sex.
WARN [2020-04-15 06:16:31] Intervals in coverage and interval.file have conflicting on/off-target annotation.
"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_linWARN [2020-04-15 06:17:05] normal.panel.vcf.file is deprecated, use mapping.bias.file instead.
e] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr1,length=249250621,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr1,length=249250621,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr2,length=243199373,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr2,length=243199373,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr3,length=198022430,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr3,length=198022430,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr4,length=191154276,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr4,length=191154276,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr5,length=180915260,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr5,length=180915260,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr6,length=171115067,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr6,length=171115067,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr7,length=159138663,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr7,length=159138663,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr8,length=146364022,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr8,length=146364022,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr9,length=141213431,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr9,length=141213431,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr10,length=135534747,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr10,length=135534747,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr11,length=135006516,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr11,length=135006516,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr12,length=133851895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr12,length=133851895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr13,length=115169878,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr13,length=115169878,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr14,length=107349540,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr14,length=107349540,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr15,length=102531392,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr15,length=102531392,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr16,length=90354753,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr16,length=90354753,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr17,length=81195210,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr17,length=81195210,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr18,length=78077248,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr18,length=78077248,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr19,length=59128983,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr19,length=59128983,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr20,length=63025520,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr20,length=63025520,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chr21,length=48129895,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr21,length=48129895,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "#FATAL [2020-04-15 06:17:20] The normal.panel.vcf.file contains only a single sample. 

FATAL [2020-04-15 06:17:20]  

FATAL [2020-04-15 06:17:20] This is most likely a user error due to invalid input data or 

FATAL [2020-04-15 06:17:20] parameters (PureCN 1.16.0). 

== testthat results  ===========================================================
[ OK: 334 | SKIPPED: 0 | WARNINGS: 10 | FAILED: 0 ]
> 
> proc.time()
   user  system elapsed 
 430.01    3.06  495.06 
#contig=<ID=chr22,length=51304566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chr22,length=51304566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrX,length=155270560,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrX,length=155270560,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrY,length=59373566,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrY,length=59373566,assembly=""hg19"">
[E::bcf_hdr_parse_line] Could not parse the header line: "##contig=<ID=chrM,length=16571,assembly=""hg19"">"
[W::bcf_hdr_parse] Could not parse header line: ##contig=<ID=chrM,length=16571,assembly=""hg19"">

Example timings

PureCN.Rcheck/examples_i386/PureCN-Ex.timings

nameusersystemelapsed
annotateTargets7.950.309.42
bootstrapResults1.440.161.60
calculateBamCoverageByInterval0.390.010.86
calculateLogRatio0.850.161.67
calculateMappingBiasVcf3.190.083.58
calculatePowerDetectSomatic2.030.512.54
calculateTangentNormal4.200.254.58
callAlterations0.430.020.44
callAlterationsFromSegmentation2.980.193.39
callAmplificationsInLowPurity46.54 0.0746.63
callCIN0.360.040.39
callLOH0.460.030.48
callMutationBurden1.570.091.79
centromeres000
correctCoverageBias1.820.031.85
createCurationFile0.530.030.56
createNormalDatabase3.20.03.2
filterIntervals22.16 0.0622.27
filterVcfBasic0.860.000.86
filterVcfMuTect0.880.000.88
filterVcfMuTect20.870.000.87
findFocal8.660.058.86
getSexFromCoverage0.220.000.22
getSexFromVcf0.320.000.32
plotAbs0.440.080.52
poolCoverage0.490.130.61
predictSomatic0.730.070.81
preprocessIntervals0.670.020.72
processMultipleSamples6.710.087.37
readCoverageFile0.230.000.24
readCurationFile0.420.060.48
readLogRatioFile0.030.000.05
readSegmentationFile0.020.000.02
runAbsoluteCN16.87 0.0516.89
segmentationCBS8.520.008.51
segmentationHclust15.67 0.0915.72
segmentationPSCBS28.81 0.2429.14
setMappingBiasVcf0.270.000.27
setPriorVcf0.230.000.23

PureCN.Rcheck/examples_x64/PureCN-Ex.timings

nameusersystemelapsed
annotateTargets5.560.235.80
bootstrapResults0.840.050.89
calculateBamCoverageByInterval0.270.000.27
calculateLogRatio0.640.000.64
calculateMappingBiasVcf3.530.014.66
calculatePowerDetectSomatic1.430.001.43
calculateTangentNormal3.740.023.75
callAlterations0.200.080.28
callAlterationsFromSegmentation1.780.111.89
callAmplificationsInLowPurity45.57 0.0645.65
callCIN0.320.080.40
callLOH0.540.040.58
callMutationBurden1.600.071.67
centromeres0.000.010.02
correctCoverageBias2.300.002.29
createCurationFile0.440.050.49
createNormalDatabase3.970.003.97
filterIntervals29.56 0.0529.65
filterVcfBasic0.970.000.97
filterVcfMuTect1.010.001.02
filterVcfMuTect20.970.000.97
findFocal8.750.008.75
getSexFromCoverage0.240.000.23
getSexFromVcf0.390.000.39
plotAbs0.400.070.49
poolCoverage0.610.000.61
predictSomatic0.820.020.84
preprocessIntervals0.780.000.79
processMultipleSamples6.970.006.96
readCoverageFile0.270.000.27
readCurationFile0.340.030.38
readLogRatioFile0.030.000.03
readSegmentationFile0.020.000.01
runAbsoluteCN19.75 0.0319.78
segmentationCBS9.330.029.35
segmentationHclust13.08 0.0313.11
segmentationPSCBS28.48 0.3428.91
setMappingBiasVcf0.290.000.28
setPriorVcf0.250.000.25