Be careful. If the wrong drive is removed, the VM will no longer boot. If you are concerned, clone the VM first before proceeding. # Power off the VM. # <code>VirtualBox</code> → <code>click a VM</code> → <code>Settings</code> → <code>Storage</code> → <code>click on DVD device symbol</code> → <code>click on disk removal symbol</code> # VirtualBox will ask: <blockquote>Are you sure you want to delete the optical drive?</blockquote> <blockquote>You will not be able to insert any optical disks or ISO images or install the Guest Additions without it!</blockquote> 4. <code>Click "Remove"</code>