1 Basics

1.1 Install chevreulProcess

R is an open-source statistical environment which can be easily modified to enhance its functionality via packages. chevreulProcess is a R package available via the Bioconductor repository for packages. R can be installed on any operating system from CRAN after which you can install chevreulProcess by using the following commands in your R session:

if (!requireNamespace("BiocManager", quietly = TRUE)) {
    install.packages("BiocManager")
}

BiocManager::install("chevreulProcess")

1.2 Required knowledge

The chevreulProcess package is designed for single-cell RNA sequencing data. The functions included within this package are derived from other packages that have implemented the infrastructure needed for RNA-seq data processing and analysis. Packages that have been instrumental in the development of chevreulProcess include, Biocpkg("SummarizedExperiment") and Biocpkg("scater").

1.3 Asking for help

R and Bioconductor have a steep learning curve so it is critical to learn where to ask for help. The Bioconductor support site is the main resource for getting help: remember to use the chevreulProcess tag and check the older posts.

2 Quick start to using chevreulProcess

The chevreulProcess package contains functions to preprocess, cluster, visualize, and perform other analyses on scRNA-seq data. It also contains a shiny app for easy visualization and analysis of scRNA data.

chvereul uses SingelCellExperiment (SCE) object type (from SingleCellExperiment) to store expression and other metadata from single-cell experiments.

This package features functions capable of:

  • Performing Clustering at a range of resolutions and Dimensional reduction of Raw Sequencing Data.
  • Visualizing scRNA data using different plotting functions.
  • Integration of multiple datasets for consistent analyses.
  • Cell cycle state regression and labeling.

library("chevreulProcess")

# Load the data
library(chevreuldata)
chevreul_sce <- human_gene_transcript_sce()
chevreul_sce
#> class: SingleCellExperiment 
#> dim: 9740 883 
#> metadata(2): markers experiment
#> assays(3): counts logcounts scaledata
#> rownames(9740): 5-8S-rRNA A2M-AS1 ... HHIP-AS1 AC117490.2
#> rowData names(0):
#> colnames(883): ds20181001-0001 ds20181001-0002 ... ds20181001-1039
#>   ds20181001-1040
#> colData names(49): orig.ident nCount_gene ... nFeature_transcript ident
#> reducedDimNames(2): PCA UMAP
#> mainExpName: gene
#> altExpNames(1): transcript

R session information.

#> R Under development (unstable) (2024-10-21 r87258)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 24.04.1 LTS
#> 
#> Matrix products: default
#> BLAS:   /home/biocbuild/bbs-3.21-bioc/R/lib/libRblas.so 
#> LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.12.0
#> 
#> locale:
#>  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
#>  [3] LC_TIME=en_GB              LC_COLLATE=C              
#>  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
#>  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
#>  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
#> [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
#> 
#> time zone: America/New_York
#> tzcode source: system (glibc)
#> 
#> attached base packages:
#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
#> [8] base     
#> 
#> other attached packages:
#>  [1] chevreuldata_0.99.16        ExperimentHub_2.15.0       
#>  [3] AnnotationHub_3.15.0        BiocFileCache_2.15.0       
#>  [5] dbplyr_2.5.0                chevreulProcess_0.99.5     
#>  [7] scater_1.35.0               ggplot2_3.5.1              
#>  [9] scuttle_1.17.0              SingleCellExperiment_1.29.1
#> [11] SummarizedExperiment_1.37.0 Biobase_2.67.0             
#> [13] GenomicRanges_1.59.1        GenomeInfoDb_1.43.1        
#> [15] IRanges_2.41.1              S4Vectors_0.45.2           
#> [17] BiocGenerics_0.53.3         generics_0.1.3             
#> [19] MatrixGenerics_1.19.0       matrixStats_1.4.1          
#> [21] BiocStyle_2.35.0           
#> 
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#>   [3] bitops_1.0-9               filelock_1.0.3            
#>   [5] tibble_3.2.1               EnsDb.Mmusculus.v79_2.99.0
#>   [7] graph_1.85.0               XML_3.99-0.17             
#>   [9] lifecycle_1.0.4            edgeR_4.5.0               
#>  [11] globals_0.16.3             lattice_0.22-6            
#>  [13] ensembldb_2.31.0           magrittr_2.0.3            
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#>  [75] pkgconfig_2.0.3            gtable_0.3.6              
#>  [77] blob_1.2.4                 XVector_0.47.0            
#>  [79] htmltools_0.5.8.1          bookdown_0.41             
#>  [81] GSEABase_1.69.0            ProtGenerics_1.39.0       
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#>  [89] rjson_0.2.23               curl_6.0.1                
#>  [91] cachem_1.1.0               GlobalOptions_0.1.2       
#>  [93] stringr_1.5.1              BiocVersion_3.21.1        
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#>  [97] AnnotationDbi_1.69.0       restfulr_0.0.15           
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#> [129] hms_1.1.3                  sparseMatrixStats_1.19.0  
#> [131] bit64_4.5.2                future_1.34.0             
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#> [135] igraph_2.1.1               memoise_2.0.1             
#> [137] bslib_0.8.0                bit_4.5.0                 
#> [139] EnrichmentBrowser_2.37.0