\name{vsn2trsf} \alias{predict,vsn-method} \title{Apply the vsn transformation to data} \description{Apply the vsn transformation to data.} \usage{ \S4method{predict}{vsn}(object, newdata, strata=object@strata, log2scale=TRUE, useDataInFit=FALSE) } \arguments{ \item{object}{An object of class \code{\linkS4class{vsn}} that contains transformation parameters and strata information, typically this is the result of a previous call to \code{vsn2}.} \item{newdata}{Object of class \code{\link[Biobase:class.ExpressionSet]{ExpressionSet}}, \code{\link[Biobase:class.NChannelSet]{NChannelSet}}, \code{\link[affy:AffyBatch-class]{AffyBatch}} (from the \code{affy} package), \code{\link[limma:rglist]{RGList}} (from the \code{limma} package), \code{matrix} or \code{numeric}, with the data to which the fit is to be applied to.} \item{strata}{Optional, a \code{factor} or \code{integer} that aligns with the rows of \code{newdata}; see the \code{strata} argument of \code{\link{vsn2}}.} \item{log2scale}{If \code{TRUE}, the data are returned on the glog scale to base 2, and an overall offset c is added (see \emph{Value} section of the \code{\link{vsn2}} manual page). If \code{FALSE}, the data are returned on the glog scale to base e, and no offset is added.} \item{useDataInFit}{If \code{TRUE}, then no transformation is attempted and the data stored in \code{object} is transferred appropriately into resulting object, which otherwise preserves the class and metadata of \code{newdata}. This option exists to increase performance in constructs like \preformatted{ fit = vsn2(x, ...) nx = predict(fit, newdata=x) } and is used, for example, in the \code{\link{justvsn}} function. } } \value{ An object typically of the same class as \code{newdata}. There are two exceptions: if \code{newdata} is an \code{\link[limma:rglist]{RGList}}, the return value is an \code{\link[Biobase:class.NChannelSet]{NChannelSet}}, and if \code{newdata} is numeric, the return value is a \code{matrix} with 1 column. } \author{Wolfgang Huber} \examples{ data("kidney") ## nb: for random subsampling, the 'subsample' argument of vsn ## provides an easier way to do this fit = vsn2(kidney[sample(nrow(kidney), 500), ]) tn = predict(fit, newdata=exprs(kidney)) }