\name{Drerio} \docType{package} \alias{BSgenome.Drerio.UCSC.danRer5-package} \alias{BSgenome.Drerio.UCSC.danRer5} \alias{Drerio} \title{Danio rerio (Zebrafish) full genome (UCSC version danRer5)} \description{ Danio rerio (Zebrafish) full genome as provided by UCSC (danRer5, Jul. 2007) and stored in Biostrings objects. } \details{ } \note{ This BSgenome data package was made from the following source data files: \preformatted{ sequences: danRer5.fa.gz, upstream1000.fa.gz, upstream2000.fa.gz and upstream5000.fa.gz files from http://hgdownload.cse.ucsc.edu/goldenPath/danRer5/bigZips/ AGAPS masks: http://hgdownload.cse.ucsc.edu/goldenPath/danRer5/database/gap.txt.gz RM masks: http://hgdownload.cse.ucsc.edu/goldenPath/danRer5/bigZips/danRer5.fa.out.gz TRF masks: http://hgdownload.cse.ucsc.edu/goldenPath/danRer5/bigZips/danRer5.trf.bed.gz } See \code{?\link[BSgenome]{BSgenomeForge}} and the BSgenomeForge vignette (\code{vignette("BSgenomeForge")}) in the BSgenome software package for how to make a BSgenome data package. } \author{H. Pages} \seealso{ \link[BSgenome]{BSgenome-class}, \link[Biostrings]{DNAString-class}, \code{\link[BSgenome]{available.genomes}}, \link[BSgenome]{BSgenomeForge} } \examples{ Drerio seqlengths(Drerio) Drerio$chr1 # same as Drerio[["chr1"]] if ("AGAPS" \%in\% masknames(Drerio)) { ## Check that the assembly gaps contain only Ns: checkOnlyNsInGaps <- function(seq) { ## Replace all masks by the inverted AGAPS mask masks(seq) <- gaps(masks(seq)["AGAPS"]) unique_letters <- uniqueLetters(seq) if (any(unique_letters != "N")) stop("assembly gaps contain more than just Ns") } ## A message will be printed each time a sequence is removed ## from the cache: options(verbose=TRUE) for (seqname in seqnames(Drerio)) { cat("Checking sequence", seqname, "... ") seq <- Drerio[[seqname]] checkOnlyNsInGaps(seq) cat("OK\n") } } ## See the GenomeSearching vignette in the BSgenome software ## package for some examples of genome-wide motif searching using ## Biostrings and the BSgenome data packages: if (interactive()) vignette("GenomeSearching", package="BSgenome") } \keyword{package} \keyword{data}