\name{Dmelanogaster} \docType{package} \alias{BSgenome.Dmelanogaster.UCSC.dm2-package} \alias{BSgenome.Dmelanogaster.UCSC.dm2} \alias{Dmelanogaster} \title{Drosophila melanogaster (Fly) full genome (UCSC version dm2)} \description{ Drosophila melanogaster (Fly) full genome as provided by UCSC (dm2, Apr. 2004) and stored in Biostrings objects. } \details{ } \note{ This BSgenome data package was made from the following source data files: \preformatted{ sequences: chromFa.zip, upstream1000.fa.gz, upstream2000.fa.gz, upstream5000.fa.gz from http://hgdownload.cse.ucsc.edu/goldenPath/dm2/bigZips/ AGAPS masks: all the chr*_gap.txt.gz files from ftp://hgdownload.cse.ucsc.edu/goldenPath/dm2/database/ RM masks: http://hgdownload.cse.ucsc.edu/goldenPath/dm2/bigZips/chromOut.zip TRF masks: http://hgdownload.cse.ucsc.edu/goldenPath/dm2/bigZips/chromTrf.zip } See \code{?\link[BSgenome]{BSgenomeForge}} and the BSgenomeForge vignette (\code{vignette("BSgenomeForge")}) in the BSgenome software package for how to make a BSgenome data package. } \author{H. Pages} \seealso{ \link[BSgenome]{BSgenome-class}, \link[Biostrings]{DNAString-class}, \code{\link[BSgenome]{available.genomes}}, \link[BSgenome]{BSgenomeForge} } \examples{ Dmelanogaster seqlengths(Dmelanogaster) Dmelanogaster$chr2L # same as Dmelanogaster[["chr2L"]] if ("AGAPS" \%in\% masknames(Dmelanogaster)) { ## Check that the assembly gaps contain only Ns: checkOnlyNsInGaps <- function(seq) { ## Replace all masks by the inverted AGAPS mask masks(seq) <- gaps(masks(seq)["AGAPS"]) unique_letters <- uniqueLetters(seq) if (any(unique_letters != "N")) stop("assembly gaps contain more than just Ns") } ## A message will be printed each time a sequence is removed ## from the cache: options(verbose=TRUE) for (seqname in seqnames(Dmelanogaster)) { cat("Checking sequence", seqname, "... ") seq <- Dmelanogaster[[seqname]] checkOnlyNsInGaps(seq) cat("OK\n") } } ## See the GenomeSearching vignette in the BSgenome software ## package for some examples of genome-wide motif searching using ## Biostrings and the BSgenome data packages: if (interactive()) vignette("GenomeSearching", package="BSgenome") } \keyword{package} \keyword{data}