\name{SolexaSet-class} \docType{class} \alias{SolexaSet-class} % constructors \alias{SolexaSet} \alias{SolexaSet,character-method} % methods \alias{detail,SolexaSet-method} \alias{laneNames,SolexaSet-method} \alias{laneNames,AnnotatedDataFrame-method} \alias{show,SolexaSet-method} % transforming methods \alias{readAligned,SolexaSet-method} \title{"SolexaSet" coordinating Solexa output locations with sample annotations} \description{ This class coordinates the file hierarchy produced by the Solexa `pipeline' with annotation data contained in an \code{\link[Biobase:class.AnnotatedDataFrame]{AnnotatedDataFrame}} (defined in the \pkg{Biobase} package). } \section{Objects from the Class}{ Objects can be created from the constructor: \code{SolexaSet(path, ...)}. \describe{ \item{path}{A \code{character(1)} vector giving the fully-qualified path to the root of the directory hierarchy associated with each Solexa flow cell, or an object of class \code{SolexaPath} (see \code{\linkS4class{SolexaPath}} for this method).} \item{...}{Additional arguments, especially \code{laneDescription}, an \code{\link[Biobase:class.AnnotatedDataFrame]{AnnotatedDataFrame}} describing the content of each of the 8 lanes in the Solexa flow cell.} } } \section{Slots}{ \code{SolexaSet} has the following slots: \describe{ \item{\code{solexaPath}:}{Object of class \code{"SolexaPath"}.} \item{\code{laneDescription}:}{Object of class \code{"AnnotatedDataFrame"}, containing information about the samples in each lane of the flow cell.} } } \section{Extends}{ Class \code{"\linkS4class{.Solexa}"}, directly. Class \code{"\linkS4class{.ShortReadBase}"}, by class ".Solexa", distance 2. } \section{Methods}{ \describe{ \item{solexaPath}{\code{signature(object = "SolexaSet")}: Return the directory paths present when this object was created as a \code{\linkS4class{SolexaPath}}.} \item{laneNames}{\code{signature(object = "SolexaSet")}: Return the names of each lane in the flow cell, currently names are simply \code{1:8}. } \item{show}{\code{signature(object = "SolexaSet")}: Briefly summarize the experiment path and lane description of the Solexa set. } \item{detail}{\code{signature(object = "SolexaSet")}: Provide additional detail on the Solexa set, including the content of \code{solexaPath} and the \code{pData} and \code{varMetadata} of \code{laneDescription}.} } Methods transforming \code{SolexaSet} objects include: \describe{ \item{readAligned}{ \code{signature(dirPath = "SolexaSet", pattern = ".*_export.txt", run, ..., filter=srFilter())}: Use \code{analysisPath(solexaPath(dirPath))[run]} as the directory path(s) and \code{pattern=".*_export.txt"} as the pattern for discovering Eland-aligned reads in the Solexa 'export' file format. Note that the default method reads \emph{all} aligned read files into a single object; often one will want to specify a pattern for each lane. Use an object of \code{\linkS4class{SRFilter}} to select specific chromosomes, strands, etc.} } } \author{Martin Morgan} \examples{ showClass("SolexaSet") showMethods(class="SolexaSet", where=getNamespace("ShortRead")) ## construct a SolexaSet sf <- system.file("extdata", package="ShortRead") df <- data.frame(Sample=c("Sample 1", "Sample 2", "Sample 3", "Sample 4", "Center-wide control", "Sample 6", "Sample 7", "Sample 8"), Genome=c(rep("hg18", 4), "phi_plus_SNPs.txt", rep("hg18", 3))) dfMeta <- data.frame(labelDescription=c("Type of sample", "Alignment genome")) adf <- new("AnnotatedDataFrame", data=df, varMetadata=dfMeta) SolexaSet(sf, adf) } \keyword{classes}