\name{read.marrayLayout} \alias{read.marrayLayout} \alias{widget.marrayLayout} \title{Create objects of class marrayLayout} \description{ This function creates objects of class \code{\link{marrayLayout}} to store layout parameters for two-color cDNA microarrays. } \usage{ read.marrayLayout(fname = NULL, ngr, ngc, nsr, nsc, pl.col = NULL, ctl.col = NULL, sub.col = NULL, notes = fname, skip, sep = "\t", quote = "\"", ...) } %- maybe also `usage' for other objects documented here. \arguments{ \item{fname}{the name of the file that stores plate and control information. This is usually a file obtained from a database.} \item{ngr}{the number of rows of grids per image.} \item{ngc}{the number of columns of grids per image.} \item{nsr}{the number of rows of spots per grid.} \item{nsc}{the number of columns of spots per grid.} \item{pl.col}{the column number in \code{fname} that contains plate information.} \item{ctl.col}{the column number in \code{fname} that contains control information.} \item{sub.col}{the column number in \code{fname} that contains full ID information.} \item{notes}{object of class character, vector of explanatory text.} \item{skip}{the number of lines of the data file to skip before beginning to read data.} \item{sep}{the field separator character. Values on each line of the file are separated by this character. The default is to read a tab delimited file.} \item{quote}{the set of quoting characters. By default, this is disable by setting `quote="\""'.} \item{\dots}{further arguments to \code{\link{scan}}.} } \details{} \value{ An object of class \code{\link{marrayLayout}}. } \references{http://www.bioconductor.org/ } \author{Jean Yang \email{yeehwa@stat.berkeley.edu}} \note{} \examples{ datadir <- system.file("swirldata", package="marray") ### Reading in control information from file skip <- grep("Row", readLines(file.path(datadir,"fish.gal"), n=100)) - 1 swirl.layout <- read.marrayLayout(fname=file.path(datadir,"fish.gal"), ngr=4, ngc=4, nsr=22, nsc=24, ctl.col=4, skip=skip) ### Setting control information. swirl.gnames <- read.marrayInfo(file.path(datadir,"fish.gal"), info.id=4:5, labels=5, skip=21) x <- maInfo(swirl.gnames)[,1] y <- rep(0, maNspots(swirl.layout)) y[x == "control"] <- 1 slot(swirl.layout, "maControls") <- as.factor(y) } \keyword{file}% at least one, from doc/KEYWORDS \keyword{connection}