\name{imageScreen} \alias{imageScreen} \title{Experiment-wide quality control plot of a cellHTS object} \description{Experiment-wide quality control plot of a scored \code{cellHTS} object.} \usage{ imageScreen(object, ar = 3/5, zrange, map = FALSE, anno) } \arguments{ \item{object}{a \code{\linkS4class{cellHTS}} object that has already been scored (i.e. \code{state(object)['scored']=TRUE}).} \item{ar}{the desired aspect ration for the image plot (i.e. number of columns per number of rows)} \item{zrange}{the range of values to be mapped into the color scale. If missing, \code{zrange} will be set to the range of the score values stored in slot \code{assayData} of \code{object}.} \item{map}{a logical value that determines whether an image map should be created using tooltips to indicate the annotation at each position. It only makes sense to set it to \code{TRUE} when the function is called from \code{writeReport} function, so the default is \code{FALSE}.} \item{anno}{optional input giving the annotation information for the mapping. It should be a vector of the same size as the total number of featured in \code{object}. See details.} } \details{ This function creates an image plot that gives an overview of the whole set of score values stored in slot \code{assayData} of a scored \code{cellHTS} object. When the annotation mapping is performed, by default, \code{anno} is set to: \enumerate{ \item (if \code{object} is annotated) The content of column named \code{GeneSymbol} or named \code{GeneID} (if the former is not available) of the \code{featureData} slot of \code{object}; \item The position within the plate, if \code{object} is not annotated. }%enumerate } \seealso{ \code{\link[cellHTS2:normalizePlates]{normalizePlates}}, \code{\link[cellHTS2:summarizeChannels]{summarizeChannels}}, \code{\link[cellHTS2:scoreReplicates]{scoreReplicates}}, \code{\link[cellHTS2:summarizeReplicates]{summarizeReplicates}}, \code{\link[cellHTS2:Data]{Data}} \code{\link[cellHTS2:writeReport]{writeReport}} } \references{ Boutros, M., Bras, L.P. and Huber, W. (2006) Analysis of cell-based RNAi screens, \emph{Genome Biology} \bold{7}, R66. } \author{Ligia Bras \email{ligia@ebi.ac.uk}} \examples{ data(KcViabSmall) x <- KcViabSmall x <- normalizePlates(x, scale="multiplicative", log=FALSE, method="median", varianceAdjust="none") x <- scoreReplicates(x, sign="-", method="zscore") x <- summarizeReplicates(x, summary="min") imageScreen(x, zrange=c(-5,5)) } \keyword{manip}