\name{preprocess} \alias{preprocess} \title{Background correct and Normalize} \description{ This function pre-processes an \code{\link[affy]{AffyBatch}}. } \usage{ preprocess(object,subset=NULL, normalize=TRUE,background=TRUE,background.method="RMA.2",normalize.method="quantile",background.param = list(),normalize.param=list(),verbosity.level=0) } %- maybe also `usage' for other objects documented here. \arguments{ \item{object}{an \code{\link[affy:AffyBatch-class]{AffyBatch}}} \item{subset}{a vector with the names of probesets to be used. If NULL then all probesets are used.} \item{normalize}{logical value. If \code{TRUE} normalize data using quantile normalization} \item{background}{logical value. If \code{TRUE} background correct using RMA background correction} \item{background.method}{name of background method to use.} \item{normalize.method}{name of normalization method to use.} \item{background.param}{list of parameters for background correction methods} \item{normalize.param}{list of parameters for normalization methods} \item{verbosity.level}{An integer specifying how much to print out. Higher values indicate more verbose. A value of 0 will print nothing} } \details{ This function carries out background correction and normalization pre-processing steps. It does not summarize to produce gene expression measures. All the same pre-processing methods supplied by \code{\link{threestep}} are supported by this function. } \value{ An \code{\link[affy]{AffyBatch}} } \author{Ben Bolstad \email{bmb@bmbolstad.com}} \references{Bolstad, BM (2004) \emph{Low Level Analysis of High-density Oligonucleotide Array Data: Background, Normalization and Summarization}. PhD Dissertation. University of California, Berkeley.} \seealso{\code{\link[affy]{expresso}}, \code{\link[affy]{rma}}} \examples{ if (require(affydata)) { data(Dilution) # should be equivalent to the bg and norm of rma() abatch.preprocessed <- preprocess(Dilution) } } \keyword{manip}