\name{bam2sig} \alias{bam2sig} \title{ bam2sig - encapsulated pipeline of finding significant expression } \description{ Reads BAM files according to annotation and produces output table processed with DESeq negative binomial test. } \usage{ bam2sig(annotlib, covdesc="covdesc", species=NULL, level="gene") } \arguments{ \item{annotlib}{Character table or data frame with colums: chr, start, end, strand, name } \item{covdesc}{Name of the file that includes BAM files (experiment description file) } \item{species}{Species name - needed for .chr.convert function - to match BAM and annotation chromosome names } \item{level}{The level of annotation for calculating the counts: gene, transcript of exon } } \value{ Output table with significant expression results, as from DESeq } \author{ Michal Okoniewski, Anna Lesniewska } \examples{ if (1==0) { all.g <- all.genes(as.vector=F) ss <- sample(1:20000, 10) genes <- as.data.frame(all.g[ss,]) deseqRes <- bam2sig("cassava.db") deseqRes[1:10,] } }