% --- Source file: man/seq.mask.Rd --- \name{plotProbe} \alias{plotProbe} \title{ Plot probes } \description{ Pairwise plot probes of a probeset. } \usage{ plotProbe(affy,probeset,probe=NA,probeXY=NA,scan=TRUE,ind,exmask="none",seqmask="none",names=FALSE) } \arguments{ \item{affy}{ An object of class \code{AffyBatch}. } \item{probeset}{Probe set name (Affymetrix ID).} \item{probe}{ Number of the main probe. } \item{probeXY}{ If probe is NA the x and y coordinates of the main probe can be given in the format 'x.y'. } \item{scan}{ If scan is 'TRUE', each probewise comparison of the probe against all other probes in this probeset will be performed seperately. If scan is 'FALSE', all plots will be plotted in one layout. The layout has 3 columns. If the number of remaining probes that the probe should be compared with is not a multiple of 3, the number of probes will be reduced to the next lower multiple of 3. } \item{ind}{ Numeric vector, with values 1 and 2, definining group assignement for samples in \code{affy}. } \item{exmask}{ Optional: an expression mask object for this affy batch. Data frame with probe information, for example first element of the output of function \code{mask}. Should contain: column 1: probe x-coordinate, column 2:probe y coordinate, column 3: probeset, column 4: quality score: values to based filtering on, probes with values smaller than cutoff are discarded. } \item{seqmask}{ Optional: a sequence mask object for this mask. } \item{names}{ If 'TRUE' , the sample names are plotted to identify each individual. } } \details{ The function \code{plotProbe} plots single probe against all other probes of its probe set. The information from the expression based mask, the sequence based mask and the test for the two plotted probes is shown. } \references{ Dannemann et al, {The effects of probe binding affinity differences on gene expression measurements and how to deal with them}. Bioinformatics 2009 } %% to do \author{ Michael Dannemann } \seealso{ \code{\link{mask}, \link{overlapExprExtMasks}, \link{prepareMaskedAffybatch}}} \examples{ data(exmask) data(AffyBatch) ## plot for one probe comparisons with other probes of the probeset ## for a random probeset availableProbesets <- as.character(unique(exmask$probes[,4])) availableProbesets ## scan the plots \dontrun{plotProbe(affy=newAffyBatch,probeset=availableProbesets[22],probe=5,scan=TRUE,ind=rep(1:2,each=10),exmask=exmask$probes,seqmask=sequenceMask)} ## scan with names=TRUE \dontrun{plotProbe(affy=newAffyBatch,probeset=availableProbesets[22],probe=5,scan=TRUE,ind=rep(1:2,each=10),exmask=exmask$probes,seqmask=sequenceMask,names=TRUE)} ## plot with given x y information \dontrun{plotProbe(affy=newAffyBatch,probeset=availableProbesets[22],probeXY="313.415",scan=TRUE,ind=rep(1:2,each=10),exmask=exmask$probes,seqmask=sequenceMask,names=TRUE)} ## all plots in one layout plotProbe(affy=newAffyBatch,probeset=availableProbesets[22],probe=5,scan=FALSE,ind=rep(1:2,each=10),exmask=exmask$probes,seqmask=sequenceMask)} \keyword{internal}