\name{MIR-spike-in} \alias{MIR-spike-in} \alias{MIR_RA} \alias{MIR.RA} \alias{MIR} \docType{data} \title{Data example} \description{ Data from a micro-RNA spike-in experiment, extracted from scanned images using Agilent Feature Extraction Software. } \usage{ data("MIR-spike-in") data("MIR_RA") } \details{ This dataset is derived from a library of synthetic RNA sequences, cor- responding to human mature miRNAs as well as in-house miRNAs with particularly similar sequences hybridized on an Agilent Human miRNA Mi- croarray 2.0. Data consist of a total of 799 miRNA species (excluding control features) for 4 samples organized in two groups A and B. Data, colected with the Agilent Feature Extraction Software, are stored in a RGList object with the following components: - MIR\$G: 'gMeanSignal' - MIR\$Gb: 'gProcessedSignal' - MIR\$gBGMedianSignal: 'gBGMedianSignal' - MIR\$targets 'targets' - MIR\$Row 'Row' - MIR\$Col 'Column' - MIR\$ProbeUID 'Probe ID' - MIR\$genes\$ControlType 'FLAG to specify the sort of feature' - MIR\$genes\$ProbeName 'Probe Name' - MIR\$genes\$GeneName 'microRNA Name' - MIR\$genes\$SystematicName 'microRNA Name' - MIR\$genes\$Description 'Description (not used)' \code{MIR.RA} holds an object of class \code{RA} obtained from using \code{estVC} on the example data. } \references{ Willenbrock H, Salomon J, Barken KIMB, Nielsen FC, Litman T. 2009. Quantitative miRNA expression analysis: Comparing microarrays with next-generation sequencing. RNA 15: 2028-2034. Data available from Genome Expression Omnibus (GEO) database under the series accession number GSE14511 } \author{Stefano Calza} \seealso{ \code{read.mir}, \code{estVC} } \keyword{datasets}