version 0.1: (2015-04-20) - Original release version 0.1.1: (2015-04-21) - Improved documentation version 0.1.2: (2015-04-23) - In 'BASiCS_Sim': change 'sum(phi)==n' by 'all.equal(sum(phi),n)' version 0.1.3: (2015-05-18) - 'plotBASiCSDispVsExp': 'log="xy"' argument added - 'BASiCS_Data': internal checks added (pre-filter of samples and transcripts) - 'BASiCS_MCMC' c++ code: Rcpp::checkUserInterrupt() added for fast user-requested interruption of MCMC sampler - S4 method 'plot' for 'BASiCS_Chain' signature: argument 'Column' replaced by 'Gene' and 'Cell' - Replacement of 'plotBASiCSNormPhi' and 'plotBASiCSNormS' by S4 method 'plot' ('BASiCS_Summary' signature) - S4 method 'plot' for 'BASiCS_Summary' signature: does not include plots for all model parameters - S4 method 'plot' for 'BASiCS_Summary' signature: does not include scatterplots of gene-specific and cell-specific model parameters (replacing 'plotBASiCSExpVsDisp') - 'BASiCS_VarianceDecomp' has been made visible to users (former 'HiddenVarDecomp' function) and output does now allow gene annotation. - 'BASiCS_Filter' function added. - Vignette has been updated according to the changes above. Instructions for installation were added. - 'BASiCS_Filter' function added. - Dependency to R (>= 3.1.0) has been added. version 0.1.4: (2015-05-21) - 'BASiCS_Denoise' function added. It produces a table of normalised and denoised expression counts (after removing the effect of technical variation). version 0.1.5: (2015-05-27) - Small typo in 'BASiCS_VarThresholdSearchHVG' and 'BASiCS_VarThresholdSearchLVG' has been fixed. version 0.1.6: (2015-06-01) - Replacement of parameter-specific 'displayChain's functions by a generic method displayChainBASiCS - Replacement of parameter-specific 'displaySummary's functions by a generic method displaySummaryBASiCS - In 'BASiCS_DetectHVG' and 'BASiCS_DetectLVG': constrain added to that prob >= 0.5 is required to detect HVG and LVG version 0.2.0: (2015-06-24) - New parametrization for mRNA content size factors $\phi_j$ that improves mixing of the MCMC algorithm (using adaptive Dirichlet proposals) - Updated vignette version 0.2.1: (2015-07-23) - Argument 'GeneNames' has been added to functions 'BASiCS_VarianceDecomp', 'BASiCS_DetectHVG', 'BASiCS_DetectLVG' so that users can specify gene labels or names that will be used for these functions's output. version 0.3.0: (2015-07-31) - New slot BacthInfo in 'BASiCS_Data' class - Batch-speficic technical variability parameters are allowed - 'BASiCS_VarianceDecomp' modified to accommodate batch membership (including graphical output) version 0.3.1: (2015-09-17) - New slot GeneNames in 'BASiCS_Data' class - Changes in the constructor `newBASiCS_Data` to allow easier construction of `BASiCS_Data` objects - Minor changes to some functions' output to use the new GeneNames slot of 'BASiCS_Data' class version 0.3.2: (2015-10-23) - Minor changes to the output of `BASiCS_MCMC` function (colnames of the elements related to the parameter $\theta$) - Addition of extra optional parameter `ls.phi0` to `BASiCS_MCMC` function. This is helpful on situations where the default value led to slow mixing to the chains related to the normalising constants $\phi_j$'s. version 0.3.3: (2015-11-05) - Same method as before but improved performance of C++ code version 0.3.4: (2015-11-11) - Fix of example code of 'newBASiCS_Data' function. Thanks to Simon Andrews for pointing out this issue. version 0.3.5: (2015-11-27) - Optional argument `PriorDelta` added to `BASiCS_MCMC` function, to allow optional use of `gamma` or `log-normal` priors for delta. - In `BASiCS_MCMC` function, change of default value of `s2.mu` to 0.5. Allows better shrinkage in situations where a gene has zero counts across all cells. - In `BASiCS_Data` class. Count matrices where a gene has zero counts across all cells are now allowed. However, a warning is returned in such case. Only use such matrices when running differential expression results. version 0.4.0: (2015-12-21) - Preliminary functions for differential expression analyses (mean and over-dispersion) have been added. version 0.4.1: (2016-02-05) - Fixed bug in vignette (usage of `newBASiCS_Data` function). Same bug has been fixed in documentation of class `BASiCS_Data`. - Optional argument 'Start' added to `BASiCS_MCMC` function to allow running chains with a variety of user-defined starting point. In general, we do not advise to use this argument as the default option has been tuned to facilitate convergence. - Updated documentation of 'BASiCS_DetectHVG' and 'BASiCS_VarianceDecomp' function (removing 'GeneNames' argument) - Updated documentation for 'BASiCS_DV_TestDE' function. - Missing @description sections added to several .Rd files. version 0.5.0: (2016-03-03) - Extended package description to include comparisons between pre-specified populations of cells - `BASiCS_DV_Data`, `BASiCS_DV_Chain` and `BASiCS_DV_Summary` classes replaced by `BASiCS_D_Data`, `BASiCS_D_Chain` and `BASiCS_D_Summary` classes, respectively. - Slot 'offset' added to `BASiCS_D_Chain` and `BASiCS_D_Summary` classes. - Slot 'probHPD' added to `BASiCS_D_Summary` class. - Creation of `CombineBASiCS_Data` function to combine 2 independent `BASiCS_Data` objects into one `BASiCS_D_Data` object. - Offset value added to the input of `show` method for `BASiCS_D_Chain` and `BASiCS_D_Summary` classes. - Creation of `CombineBASiCS_Chain` function to combine 2 independent `BASiCS_Chain` objects into one `BASiCS_D_Chain` object. - 'GeneNames' slot added to `BASiCS_Data` class version 0.5.1: (2016-03-07) - In function `BASiCS_D_TestDE`. Probability threshold set to 0.95 when EFDR fails to calibrate (simulations under the null) version 0.5.2: (2016-03-07) - In function `BASiCS_D_TestDE`. Probability threshold set to 0.90 when EFDR fails to calibrate (simulations under the null) - Fixed version number in description file. version 0.5.3: (2016-03-09) - Slots `BatchInfoTest` and `BatchInfoRef` added to `BASiCS_D_Data` class. - `newBASiCS_D_Data` function modified to incorporate `BatchInfoTest` and `BatchInfoRef` slots. - `CombineBASiCS_Data` function modified to incorporate `BatchInfoTest` and `BatchInfoRef` slots. - `show` method for `BASiCS_D_Data` class modified to incorporate `BatchInfoTest` and `BatchInfoRef` slots. - `GeneNames` argument missing in `makeExampleBASiCS_D_Data` has been added. - Slots `thetaTest` and `thetaRef` of `BASiCS_D_Chain` objects modified to accept matrices (necessary to allow multiple batches). version 0.5.4: (2016-04-15) - Minor fixes to the documentation files - Argument `GenesSelect` was added to function `BASiCS_D_TestDE` to allow user-defined lists of genes to be included in the comparison between cell types. version 0.5.5: (2016-04-18) - Small fix when checking the validity of a `BASiCS_D_Data` object. Thanks to Nils Eling. - Fixed issue with documentation files. version 0.5.6: (2016-04-19) - Fixed message in `BASiCS_D_Test` function. This does not affect functionality. Only the message that is printed in the console. version 0.5.7: (2016-04-27) - Welcome message added version 0.5.8: (2016-05-18) - After profiling of c++ functions, minor edits to improve memory usage. These do not affect user interface - Small change in funtion 'makeExampleBASiCS_Data' to avoid 'NOTICE' message version 0.5.9: (2016-05-19) - Dependency to 'scran' added - 'HiddenBASiCS_MCMC_Start' modified to use 'scran' estimates as starting values. This allows faster convergence. - Updated 'README.md' file to build and access vignette during installation version 0.5.10: (2016-05-20) - Updated bug in 'HiddenBASiCS_MCMC_Start' function (thanks to Joanna Dreux for noticing this issue) version 0.5.11: (2016-05-24) - 'importMethodsFrom(scran, computeSumFactors)' added to NAMESPACE version 0.6.1: (2016-05-25) - Argument 'WithSpikes' added to 'makeExampleBASiCS_Data', generating an example data with no spikes - Modifications to validity checks of 'BASiCS_Data' class to allow no spikes case (SpikeInput = 1) - 'show' methods for 'BASiCS_Data' class modified to deal with no spike case - Checks for 'BASiCS_Data' object modified to require: either spikes or batches - 'BASiCS_MCMC' modified for no spikes case (if/else statement only, not yet functional) - 'HiddenBASiCS_MCMC_Start' modified for no spikes case - Function 'HiddenBASiCS_MCMCcppNoSpikes' added. Update for 'phi' is pending. - Small vignette file for no spikes case added version 0.6.2: (2016-05-31) - Template for 'phiUpdateNoSpikes' has been added. - Minor change in 'phiUpdate' for all 'HiddenBASiCS_MCMCcpp' functions (to avoid re-writing phi0) - Faster adaptation steps for 'HiddenBASiCS_MCMCcppBatch' 'HiddenBASiCS_MCMCcppNoSpikes' - 'HiddenBASiCS_MCMCcppNoSpikes' completed for testing stage (not ready for users) version 0.6.3: (2016-05-31) - Small update in wellcome message. version 0.6.4: (2016-06-01) - Safety checks added to 'phiUpdateNoSpikes' version 0.6.5: (2016-06-8) - Modified identifiability constrains for no spikes case. version 0.6.6: (2016-07-27) - First working version of no spikes case (constrained mu's) version 0.6.7: (2016-07-28) - Minor changes in the validity checks of 'BASiCS_D_Data' objects version 0.6.8: (2016-07-28) - Sequential updates for mu in MCMC (no spikes case only) version 0.6.9: (2016-08-01) - Dirichlet-based proposals for mu in MCMC (no spikes case only) version 0.7.0: (2016-08-02) - Sequential updates for mu in MCMC (no spikes case only) version 0.7.1: (2016-08-05) - Random reference in sequential updates for mu (no spikes case only) version 0.7.2: (2016-08-05) - Same as v 0.7.2 but more efficient version 0.7.3: (2016-08-08) - Reference set to be the gene closest to the average (no spikes case only) version 0.7.4: (2016-08-09) - Modified constrain to include 'expressed' genes only (no spikes case only) version 0.7.5: (2016-08-10) - Modified constrain to include 'expressed' genes only (no spikes case only) version 0.7.6: (2016-08-11) - As v 0.7.5 but with extra argument for constrain limit (no spikes case only) version 0.7.7: (2016-08-12) - ConstrainType = 3 implemented (no spikes case only) version 0.7.8: (2016-08-15) - ConstrainType = 4 implemented (no spikes case only) version 0.7.9: (2016-10-31) - C++ code cleaned regarding parameter updates (no spikes case) - Updated email in description file and welcome message - 'BASiCS_MCMC' modified so that "log-normal" is the default value for PriorDelta version 0.7.10: (2016-10-31) - Minor changes in 'BASiCS_MCMC' to have more meaningful variable names - Minor changes in 'BASiCS_MCMC' to make no-spikes case functional version 0.7.11: (2016-10-31) - Comments added to clarify no spikes case in 'BASiCS_MCMC' function. version 0.7.12: (2016-11-01) - Change in defaul value of 'PriorDelta' (v 0.7.9) has been reverted for reproducibility issues. Message added to the start of the function so that users are aware of making the change. - PriorDelta = 'gamma' is now allowed for no spikes case version 0.7.13: (2016-11-02) - Typo on the arguments of 'HiddenBASiCS_MCMCcppNoSpikes' function has been fixed ('Constrain' instead of 'ConstrainType') - Validity check '(y(i) > 1e-3)' moved later on in 'deltaUpdateNoSpikes' to avoid breaking the code when it is not really necessary! version 0.7.14: (2016-11-03) - Debug message removed from 'muUpdateNoSpikes' function. version 0.7.15: (2016-11-07) - ConstrainType = 1 (untrimmed) re-introduced for no-spike case (illustration purposes only) - RefGene removed from AR report - Added console report of RefGene choice version 0.7.16: (2016-11-07) - ConstrainType = 3 (stochastic ref) re-introduced for no-spikes case version 0.7.17: (2016-11-07) - Error fixed in the definition of RefGenes version 0.7.18: (2016-11-08) - Function 'BASiCS_LoadChain' added to simplify the loading of pre-computed chains - Imports from 'testthat' library added to NAMESPACE file - Small changes in 'BASiCS_D' classes to allow offset version 0.7.19: (2016-11-14) - RefGene information to be stored as .txt file version 0.7.20: (2016-11-14) - 'newBASiCS_Data' function modified to allow no spikes case version 0.7.21: (2016-11-15) - 'ConstrainType' = 4, 5 added (exclude genes with capture in less than 'ConstrainLimit'100% of the cells) version 0.7.22: (2016-11-16) - Fixed bug for 'ConstrainType' = 5 (affects no spike case only) version 0.7.23: (2016-11-21) - Cleaning-up of identifiability constrain options for the no spike case version 0.7.24: (2016-11-23) - Median changed to mean when calculating identifiability constrain for the no spike case version 0.7.25: (2017-01-11) - Fixed typo in vignette file (thanks to Dmitriy Zhukov for pointing this out) version 0.7.26: (2017-02-09) - Update of 'BASiCS_Filter' function to include a 'BatchInfo' argument (thanks to Dmitriy Zhukov for suggesting this) - Vignette file updated accordingly. - Update of 'newBASiCS_Data' function to prevent issues related to unused levels when the 'BatchInfo' argument is set as a 'factor' (thanks to Kevin Rue-Albrecht for pointing out this issue) version 0.7.27: (2017-05-09) - Bug fixed in 'BASiCS_Filter' function to stop the code crashing when there are genes with zero counts across all cells. version 0.7.28: (2017-07-21) - Fixed dependency to 'data.table' to prevent errors in 'BASiCS_LoadChain' function(thanks to Yongchao Ge for pointing out this issue). This change affects the DESCRIPTION file as well as the 'BASiCS_LoadChain' function. version 0.7.29: (2017-07-24) - 'BASiCS:::HiddenBASiCS_MCMC_Start' function modified regarding the definition of starting values for mu (to avoid adding +1 when it's not necessary) - 'BASiCS:::HiddenBASiCS_MCMC_Start' modified to have better starting values for delta (this is based on eq 2 from Vallejos et al, 2016) version 0.7.30: (2017-07-26) - Typo fixed in 'BASiCS:::HiddenBASiCS_MCMC_Start' function (thanks to @bdulken for pointing out this issue) version 1.0.0: (2017-07-29) - Initial big merge with @nilseling changes (in preparation for Bioconductor submission) - For reference, here is a summary of the changes implemented by @nilseling: * Creation of individual .R for each R function (but all 'Hidden' functions are in one file) * BASiCS_Data class replaced by SummarizedExperiment class (Bioconductor) * Contact email updated to 'cvallejos@@turing.ac.uk' * Methods for BASiCS_Data and BASiCS_D_Data objects removed (these classes no longer exist) * Adjust newBASiCS_Data function to create a SummarizedExperiment object * 'cat' calls replaced by 'message' calls * Clean up of BASiCS_D_TestDE function (removed unused args) * Clean up of BASiCS_DetectHVG_LVG function (using SummarizedExperiment class) * Clean up of BASiCS_MCMC function (using SummarizedExperiment class) * Clean up of BASiCS_Sim function (using SummarizedExperiment class; extra arg 'muSpikes') * Clean up of BASiCS_DetectHVG_LVG function (using SummarizedExperiment class) * Clean up of BASiCS_VarianceDecomp function (using SummarizedExperiment class) * Clean up of BASiCS_VarThresholdSearchHVG function (using SummarizedExperiment class) * Clean up of BASiCS_DenoisedCounts function (using SummarizedExperiment class) * Clean up of BASiCS_DenoisedRates function (using SummarizedExperiment class) * Clean up of HiddenBASiCS_MCMC_Start function (using SummarizedExperiment class) * Clean up of makeExampleBASiCS_Data function (using SummarizedExperiment class) * Removed methods associated to BASiCS_D_MCMC class (no longer in use) * NAMESPACE + Rd files updated accordingly * DESCRIPTION file updated accordingly * Vignette file updated accordingly version 1.0.1: (2017-07-31) - Minor changes function-by-function (revision of documentation + minor bugs) * newBASiCS_Data : added ref to GB paper + default par value doc + Nils as author * BASiCS_Filter : added ref to GB paper + default par value doc * makeExampleBASiCS_Data : added ref to GB paper + default par value doc + Nils as author * BASiCS_MCMC : added ref to GB paper + Nils as author + fix print of system.time + updated examples - Major changes function by function * makeExampleBASiCS_Data : `Case1` param replaced by `Example` (vignette + unit test updated accordingly) * BASiCS_MCMC : default value for PriorDelta set to 'log-normal' * HiddenBASiCS_MCMC_Start : new starting values for delta (as in main repo) version 1.0.2: (2017-08-01) - Minor changes function-by-function (revision of documentation + minor bugs) * BASiCS_MCMC : 'MCMC_Output' replaced by 'Chain' in examples - Major changes function-by-function * BASiCS_MCMC : chains stored a single .Rds files, with BASiCS_Chain format (rather than separate .txt files) * BASiCS_LoadChain : updated accordingly * BASiCS_DetectHVG : evidence threhold to be defined by EFDR only - Vignette updated accordingly version 1.0.3: (2017-08-01) - Minor changes function-by-function (revision of documentation + minor bugs) * BASiCS_DetectHVG / LVG: added vertical/horizontal lines in EFDR/EFND plot, subtasks moved to hidden functions (avoids repeated code) - Major changes function-by-function * BASiCS_DetectLVG : evidence threhold to be defined by EFDR only * BASiCS_DetectHVG / LVG: fix on default prob threshold = 0.5 when optimal is < 0.5 - Examples in BASiCS_MCMC updated accordingly - Vignette updated accordingly version 1.0.4: (2017-08-01) - Minor changes function-by-function (revision of documentation + minor bugs) * BASiCS_VarThresholdSearchHVG / LVG : EFDR criteria updated as in BASiCS_DetectHVG / LVG * BASiCS_TestDE : added examples - Major changes function-by-function * BASiCS_D_TestDE : no longer in use message added - Examples in BASiCS_MCMC updated accordingly - Vignette updated accordingly - Updated DESCRIPTION file (authors and description) - Updated welcome message (with link to new wiki) version 1.0.5: (2017-08-01) - Major changes function-by-function * BASiCS_TestDE : plotting functionality for offset added - ACF plot added to BASiCS_Chain plots version 1.0.6: (2017-08-02) - 'matrixStats' added within 'Imports' in DESCRIPTION (to use 'colMedians' function) - Minor changes function-by-function (revision of documentation + minor bugs) * BASiCS_TestDE : Improved offset plots - Major changes function-by-function * BASiCS_TestDE : Ref/Test notation changed to Group1/2 + output table names changed (to Mean/Disp) + offset corrected objects as output + separated output tables Mean/Disp + post prob threshold moved to hidden function (avoids duplicated code) version 1.0.7: (2017-08-03) - Multiple changes in BASiCS_TestDE: * Added examples of table viewing in doc * Added chain extracts to run examples * Results for differential dispersion to exclude differential mean genes * EFDR / EFNR control plot added * MA plots added - Main hidden functions moved into individual files - 'graphics' + 'KernSmooth' added as dependence to use 'smoothScatter' - 'smooth' argument changed to 'SmoothPlot' in plot functions/methods - Added reference :: to basic functions (e.g. boxplot, var, acf, etc) - devtools::check() OK - 1 warning : BiocStyle in vignette version 1.0.8: (2017-08-03) - 'Data' arg removed from args in: 'HiddenHeaderDetectHVG_LVG' + 'HiddenVarDecomp' + 'BASiCS_DetectHVG' + 'BASiCS_DetectLVG' + 'BASiCS_VarThresholdSearchHVG' + 'BASiCS_VarThresholdSearchLVG' - 'object' arg renamed as 'Chain' in 'BASiCS_DetectHVG' + 'BASiCS_DetectLVG' + 'BASiCS_VarThresholdSearchHVG' + 'BASiCS_VarThresholdSearchLVG' - Examples in BASiCS_MCMC updated to use built-in chain - Removal of 'smoothPlot' usage in plots for 'BASiCS_Summary' class (avoids issues with log) - Volcano plots added in BASiCS_TestDE function - Resolved issues with log scale in 'smoothScatter' - Minor bugs fixed in BASiCS_TestDE - 'EviThreshold' arg replaced by 'ProbThreshold' in 'BASiCS_HVG' + 'BASiCS_LVG' - Vignette updated accordingly version 1.0.9: (2017-08-04) - newBASiCS_Data : added check to ensure SpikeInfo is a data.frame - BASiCS_TestDE : default value for EpsilonM & EpsilonD has changed - Vignette + examples updated accordingly version 1.0.10: (2017-08-06) - BASiCS_TestDE : changes in output messages - Gene name added in plot method for BASiCS_Chain objects version 1.0.11: (2017-08-06) - BASiCS_VarianceDecomp : small bugs fixed (unused Data object) - colnames fixed on cell-specific slots ChainSC and ChainRNA - Rcpp::checkUserInterrupt() activated in C++ code for interruptions - 'grDevices' added as dependency version 1.0.11: (2017-08-13) - 'WithSpikes' argument removed from 'BASiCS_LoadChain' function version 1.0.12: (2017-08-14) - 'SummarizedExperiment' class replaced by 'SingleCellExperiment' - Updated vignette version 1.0.13: (2017-08-15) - Minor changes to documentation - Minor changes to vignette version 1.1.0: (2017-08-16) - Minor changes required to pass BiocCheck (used formatR and BiocChecks) version 0.99.0: (2017-08-28) - Minor changes to complete Bioconductor submission checklist - Version re-numbered according to Bioconductor guidelines version 0.99.2: (2017-09-18)- After @nturaga review - 'newBASiCS_Data': format + checks moved to `HiddenChecksBASiCS_Data` function - `HiddenChecksBASiCS_Data`: use of colMeans2, rowMeans2 - Format in welcome message - 'newBASiCS_Chain': format version 0.99.3: (2017-09-21) - Extra unit test (parameter estimates for a given seed) - Format + vectorisation in `HiddenThresholdSearchTestDE` function - `HiddenProbDE` removed as no longer needed - Additional unit test for differential test added - Format for `BASiCS_TestDE` function - Format for `HiddenBASiCS_MCMC_Start` function - Format for `HiddenChecksBASiCS` function - Format for `BASiCS_D_TestDE` - Format for all S4 methods version 0.99.3: (2017-09-21) - 2^ added to FC in `BASiCS_TestDE` TableDisp - Format for data documentation - Format for classes definition - Format + vectorization for `BASiCS_Filter` - Format for `BASiCS_LoadChain` version 0.99.3: (2017-09-22) - Removal of second example in `makeExampleBASiCS_Data` (no longer required due to DataSC, DataRNA) - Format for `makeExampleBASiCS_Data` - Format for `BASiCS_Sim` version 0.99.3: (2017-09-22) - Format + matrixStats usage in `BASiCS_VarianceDecomp` - Unit test for HVG/LVG detection added - Minor bug in `BASiCS_TestDE` resolved (related to colMeans2 use) - Format + matrixStats usage in `HiddenVarDecomp` - `message` replaced by `cat` in Methods.R - Removal of no-longer-needed Hidden functions - Format in `HiddenHeaderDetectHVG_LVG` - Format in `HiddenPlot1DetectHVG_LVG` + `HiddenPlot2DetectHVG_LVG` - Format + matrixStats usage in `BASiCS_DetectHVG` + `BASiCS_DetectLVG` - Format in `BASiCS_VarThresholdSearchHVG` - Format in `HiddenThresholdSearchDetectHVG_LVG` version 0.99.3: (2017-09-22) - Calculations in `BASiCS_DenoisedRates` moved to C++ to increase speed version 0.99.3: (2017-09-24) - Format in `BASiCS_DenoisedCounts` - Edits in `HiddenBASiCS_MCMCcpp` and associated C++ functions * Dimensions for mu storage upto q0 only * Creation of generic storage values * Formating of long lines * Removal of no-longer-required functions and debug lines version 0.99.3: (2017-09-25) - Edits in `HiddenBASiCS_MCMCcpp` and associated C++ functions * Optimization of MH updates version 0.99.3: (2017-09-25) - Edits in `HiddenBASiCS_MCMCcpp` and associated C++ functions * Batch & no-batch versions merged in a single function * Avoids double transponse for chain storage version 0.99.4: (2017-09-25) - Format in `BASiCS_MCMC` - Minor bug fixed in `BASiCS_DetectLVG` plots - Added examples in `BASiCS_DenoisedCounts` + `BASiCS_DenoisedRates` - Removal of `Example` argument in `makeExampleBASiCS_D_Data` - Format in vignette - R CMD CHECK passed - R CMD BiocCheck passed version 0.99.5: (2017-09-26) - Change of default `PriorDelta` value in `BASiCS_MCMC` - Unit tests modified accordingly version 0.99.7: (2017-10-05) - After @nturaga review - `inst` folder has been deleted - Removal of `...` arg in `segments` - `plot` method for `BASiCS_Summary` version 0.99.8: (2017-10-10) - version bump to trigger a new Bioconductor build version 0.99.9: (2017-10-16) - Updated URL and BugReports in DESCRIPTION version 0.99.10: (2017-10-17) - Updated README with BioC installation instructions and @nturaga acknowledgment version 0.99.12: (2017-10-17) - Update of NEWS with changes by @neling - Small typo fix in class validity from @neling last commit - Changes in `BASiCS_Chain` and `BASiCS_Summary` classess to contain a single `list` slot where all parameters are stored. This allows greater flexibility to incorporate ongoing developments of BASiCS but does not affect user interface. - `BASiCS_DenoisedCounts`, `BASiCS_DenoisedRates`, `BASiCS_DetectHVG`, `BASiCS_DetectLVG`, `BASiCS_MCMC`, `BASiCS_TestDE`, `HiddenVarDecomp`, `newBASiCS_Chain` functions and all methods updated accordingly - `ChainSC` and `ChainRNA` example objects updated accordingly - Unit tests updated accordingly - Wiki page updated accordingly - `updateObject` generic method imported from `BiocGenerics` - New generic methods definition moved to AllGenerics.R - Change in class definition to store current packageVersion - `BASiCS_LoadChain` function updated to deal with old class definition and .txt storage. - Shorter welcome message. - Sanity checks in `BASiCS_TestDE` moved to separate file. - Updated code indentation in `BASiCS_TestDE` - Warning in `BASiCS_DetectHVG` and `BASiCS_DetectLVG` when user provides posterior probability thresholds as input - Unused argument removed from `HiddenEFDR` and `HiddenEFNR` functions - Minor changes to pass R CMD check - Check library passes R CMD BiocCheck - Tidy up NAMESPACE - Tidy up NEWS - Merge Devel_class into master branch version 0.99.13: (2017-10-17) - @neling email added to DESCRIPTION + author contributions version 0.99.14: (2017-10-19) - Added checks to ensure input `SingleCellExperiment` object has all info version 1.0.0: (2017-10-30) - Bioconductor 3.6 release version 1.1.0: (2017-10-30) - Version number bump after bioconductor 3.6 release version 1.1.1: (2017-11-08) - 'ggplot2' added to imports - Default value for SpikeInfo changed to NULL in `newBASiCS_Data` function - Cleaning of `HiddenBASiCS_MCMCcppNoSpikes` - Cleaning of full conditionals for no-spikes implementation - Fixed bug in stochastic reference implementation - Added unit test for no-spikes case (estimation) - Minor tyle changes to merge regression case - 'Eta' parameter removed from `Summary` method (regression case only) - Corrected HPD interval calculation for epsilon and sigma2 (regression case) - Added mark for 'ExcludedFromRegression' genes in `BASiCS_TestDE` version 1.1.2: (2017-11-08) - Skeleton for sampler with integrated out s version 1.1.3: (2017-11-08) - Collapsed sampler implemented (to be tested) - Unit tests reverted to original case - Version number bump - Store `TableRef` on the `StoreDir` directory (no-spike case only) version 1.1.4: (2017-11-12) - Only include genes that are expressed in at least 2 cells per condition for differential residual dispersion testing (regression case only) version 1.1.5: (2017-11-15) - Removal of collapsed sampler prototype (from C++ and R) - moved to `CollapsedSampler` branch - Number of genes used for stochastic reference increased to 10% of genes (no spikes only) version 1.1.6: (2017-11-15) - Added prototype for regression + no-spikes sampler version 1.1.7: (2017-11-20) - Nils added as author in cpp file - Updated unit test for no-spikes case (changes due to change in stoch ref) - Change in `BASiCS_MCMC` so that only biological counts go to C++ - Added factorizations in `muUpdateReg` and `deltaUpdateReg` (C++ code) version 1.1.8: (2017-11-21) - `muUpdateRegNoSpikes` and `deltaUpdateRegNoSpikes` added to C++ code - Clean-up of C++ code (repeated debug checks made into function) - `lambdaUpdateReg` created as a separate function - Vectorization of calculations related to regression implementation - Global quantities (e.g. inv(V0)) taken outside the look (regression case) - `sigma2UpdateReg` created as a separate function - `betaUpdateReg` created as a separate function - Fixed typo in sigma2 updates (regression case); unit test updated accordingly version 1.1.9: (2017-11-24) - `means` only updated when needed (regression case); unit test updated accordingly - `deltaUpdateNoSpikes` + `deltaUpdateRegNoSpikes` removed as no longer required - Simplification of terms in `deltaUpdateReg` - Small changes to unify code format in C++ file - Notation change: `phi` replaced by `s` in no-spikes implementation - Unit test changed accordingly (no-spike) - Minor style changes in `BASiCS_MCMC` - `BASiCS_MCMC` function broken into smaller functions (easier to read). This creates the following hidden functions: `HiddenBASiCS_MCMC_InputCheck`, `HiddenBASiCS_MCMC_ExtraArgs`, `HiddenBASiCS_MCMC_NoSpikesParams`, `HiddenBASiCS_MCMC_OutputStore` and `HiddenBASiCS_MCMC_RefFreqStore` - Merge between no-spikes and regression case completed (code to be tested) - Minor change in storage of reference frequency (no spikes only) version 1.1.10: (2017-11-27) - `HiddenBASiCS_MCMC_Start` updated to remove regression-related hyper-params - `HiddenBASiCS_MCMC_ExtraArgs` updated to include regression-related hyper-par - `BASiCS_MCMC` modified accordingly - Storage of adaptive variances fixed for the no-spike case version 1.1.11: (2017-11-24) - Minor typo in the call to `HiddenBASiCS_MCMCcppNoSpikes` has been resolved version 1.1.12: (2017-11-28) - Additional changes to improve usage of no-spikes case version 1.1.13: (2017-11-28) - Fixed storage of `RefGene` in no-spikes when `StochasticRef = FALSE` version 1.1.14: (2017-11-29) - Minor bug resolved for no-spikes case when original data has spikes - New unit test to assess that case version 1.1.15: (2017-11-29) - Typo fixed in `AtLeast2Cells` (`BASiCS_MCMC` function) definition for no-spikes + regression case when original data has spikes version 1.1.16: (2017-11-29) - Change in `BASiCS_MCMC` no-spikes so that only genes with zero total counts are excluded from the identifiability constrain version 1.1.17: (2017-12-07) - Stop scaling of size factors for no-spikes case (`HiddenBASiCS_MCMC_Start`) - Unit testing updated accordingly - Extra unit test to ensure that no-spikes results match for objects with/without spikes when setting `WithSpikes = FALSE` - Modified constrain for no-spikes cases (to deal with non-zero genes) - Default value of `ConstrainType` set to 1 in `BASiCS_MCMC` (no-spikes only) version 1.1.18: (2017-12-08) - Minor typo in `HiddenBASiCS_MCMC_NoSpikesParam` resolved version 1.1.19: (2017-12-09) - extended vignette to describe regression and non-spike case - changed markdown to rmarkdown engine to compile vignette to include table of contents version 1.1.20: (2017-12-11) - Updated dpi option in vignette version 1.1.21: (2017-12-13) - `ConstrainProp` added as an optional argument for `BASiCS_MCMC` (no-spikes) version 1.1.22: (2017-12-15) - Documentation on ChainSCReg and ChainRNAReg datasets added - Fixed showFit method - Updated dpi option in vignette to reduce .html size - Default value for `ConstrainProp` set to 0.20 (no-spikes) - Unit tests updated accordingly (no-spikes) version 1.1.23: (2017-12-18) - Minor improvements to documentation version 1.1.24: (2017-12-18) - Reduced no of iterations in unit test for quicker testing - library `hexbin` added to imports (required for `showFit`) - Vignette edits (simplification of Quick Start) version 1.1.26: (2017-12-20) - Vignette bibliography moved to .bib file - Added diagram to summarise different implementations - Reduced size of example objects - Unit tests updated accordingly - `subset` method created for `BASiCS_Chain` objects - Removal of parameter `lambda` from `BASiCS_Chain` objects (for storage) - R/Methods.R + R/Classes.R updated accordingly - `newBASiCS_Chain` updated accordingly. - Code format for `showFit` version 1.1.27: (2017-12-20) - Minor typo resolved in `displayChainBASiCS` method - Reduced size of example objects - Unit tests updated accordingly - In `BASiCS_TestDE` function: `PsiE` argument renamed as `EpsilonR` version 1.1.28: (2017-12-20) - Vignette header as in BiocStyle - In `BASiCS_TestDE` function: `ProbThresholdE` argument renamed as `ProbThresholdR` - In `BASiCS_TestDE` function: `ProbThresholdE` argument renamed as `ProbThresholdR` - Updated documentation for example `BASiCS_Chain` objects - Minor additional changes to the documentation version 1.1.29: (2017-12-21) - `showFit` generic renamed as `BASiCS_showFit` (to avoids potential conflicts) version 1.1.30: (2018-01-22) - Commit to triger a new built version 1.1.31: (2018-01-22) - Updated documentation for `BASiCS_showFit` method version 1.1.32: (2018-01-29) - Fix to ensure that `BASiCS_MCMC` function adds cell labels for $\phi$ - `makeExampleBASiCS_Data` now produces valid `colnames` (cell labels) - Fixed colnames of $\phi$ parameter in all data examples - Extended validity test for `BASiCS_Chain` class - `rownames` and `colnames` methods created for `BASiCS_Chain` class - Added example and unit test for `BASiCS_VarianceDecomp` function version 1.1.33: (2018-01-30) - In `HiddenBASiCS_MCMC_Start`, `positive = TRUE` added to `scran::computeSumFactors` when estimated size factor contain invalid values. Thanks to Mike Morgan for suggesting this solution. version 1.1.34: (2018-01-31) - `HiddenBASiCS_MCMC_Start` edited to account for new default parameter values in `scran::computeSumFactors` call - Unit tests updated accordingly - Imports/Suggests packages are now in alphabetic order - Minor style changes suggested by BiocCheck version 1.1.35: (2018-03-18) - New unit tests (more modular): example data, starting values MCMC, MCMC sampler for fixed starting values (spikes/no-spikes; regression/no-regression) version 1.1.36: (2018-03-18) - Fixed bug in `newBASiCS_Data` to define a default value for `Tech` when spike-ins are not available. Thanks to Muad Abd El Hay (@Cumol) for pointing out this issue. version 1.1.37: (2018-03-20) - `phi` removed from the output of `BASiCS_MCMC` for no-spikes case - Unit tests updated accordingly - Checks for parameter names added to unit tests version 1.1.38: (2018-03-20) - Validity checks updated for `BASiCS_Chain` and `BASiCS_Summary` classes. This accounts for different parameter configurations - `Summary` method to exclude `RefFreq` for the no-spikes case - Unit tests fo no-spikes case updated accordingly version 1.1.39: (2018-03-20) - Denoised counts/rates added to unit test for regression case - `BASiCS_DenoisedCounts` adapted for no-spikes case - `BASiCS_DenoisedRates` adapted for no-spikes case version 1.1.40: (2018-03-20) - Fixes small bug in `BASiCS_DenoisedRates`, no-spikes case - Adds denoised rates/counts check to unit tests for no-spikes case version 1.1.41: (2018-03-20) - `BASiCS_MCMC`: Default value for `WithSpikes` set as FALSE when there are no spike-ins in available in the data version 1.1.42: (2018-03-20) - `OrderVariable` default value set as "GeneIndex" in `BASiCS_TestDE` - Fixed usage of `OrderVariable` in `BASiCS_TestDE` regression case - Associated unit test updated accordingly - Missing denoised rates/counts check added to unit tests for no-spikes case - CITATION file has been added version 1.1.43: (2018-03-20) - Batch information moved to `colData` instead of `metadata` when building a `SingleCellExperiment` object via the `newBASiCS_Data` function. This facilitates data subsetting. - `BASiCS_MCMC` and `HiddenBASiCS_MCMC_InputCheck` updated accordingly - `SummarizedExperiment::colData` and `S4Vectors::DataFrame` added as imports in NAMESPACE version 1.1.44: (2018-03-21) - `HiddenVarDecomp` adapted to no-spikes case - Unit test for `BASiCS_VarDecomp` expanded accordingly - Specification of `colnames` and `rownames` added to `newBASiCS_Data` - `colnames` and `rownames` are now specified as part of the output of `BASiCS_DenoisedCounts` and `BASiCS_DenoisedRates` version 1.1.45: (2018-03-21) - Messages within C++ code moved to functions version 1.1.46: (2018-03-21) - Split C++ code into individual functions - One file per main function and individual files for utility function - Created header files for main functions and utility functions version 1.1.47: (2018-03-21) - Reduced number of genes in data examples - Unit test updated accordingly - Nils added as maintainer in DESCRIPTION version 1.1.48: (2018-03-22) - Excluded epsilon object and RefFreq object from validity test in Chain Class version 1.1.49: (2018-03-22) - Fixed link to `BASiCS_Chain` documentation in `BASiCS_showFit` doc version 1.1.50: (2018-03-26) - Removed OffSet correction for phi in the BASiCS_TestDE function version 1.1.51: (2018-03-26) - Adjusted BASiCS_TestDE function to output data.frames containing same genes. version 1.1.52: (2018-04-09) - Added extra sentence to vignette describing the BASiCS_Data generation when SpikeInfo is not available version 1.1.53: (2018-04-13) - Fixed bug in plotting for TestDE function - Added extra code in vignette to construct BASiCS_Data with no spikes - Added plots to show changes in residual variability during testing version 1.1.54: (2018-04-15) - CITATION file updated to include latest BASiCS bioRxiv preprint version 1.1.55: (2018-04-16) - Speed-up of `BASiCS_TestDE` by avoiding unnecessary calculations version 1.1.56: (2018-04-16) - Minor changes in `BASiCS_MCMCcppNoSpikes.cpp` to avoid warnings in BioC version 1.1.57: (2018-04-16) - Minor changes in NEWS file version 1.1.58: (2018-04-19) - Updates in vignette to fully incorporate the regression and non-spike BASiCS models. version 1.1.59: (2018-04-19) - Documentation of `RefFreq` in `BASiCS_Chain` class. version 1.1.60: (2018-04-19) - Updated DESCRIPTION file to introduce residual over-dispersion testing version 1.1.61: (2018-04-19) - INCOMPLETE WORK - `Regression` is now a compulsory parameter in `BASiCS_MCMC`, hidden functions and SOME unit tests updated accordingly. version 1.1.62: (2018-04-21) - `Regression` is now a compulsory parameter in `BASiCS_MCMC`, hidden functions, documentation and unit tests updated accordingly. - Minor updates in vignette to clarify default parameter in `newBASiCS_Data` - `BASiCS_Filter` adapted to no-spikes case and minor bug resolved - Added unit test for `BASiCS_Filter` - Minor bug fixed for no-spikes case in `BASiCS_DenoisedCounts` version 1.1.63: (2018-04-27) - Extra warning message added when `scran` normalisation fails to produce positive size factors version 1.3.1: (2018-05-21) - Unit tests created for selected C++ functions (`Hidden_rDirichlet` and `Hidden_muUpdate`) - Updated unit tests in spike-in parameter estimation to have tolerance (TEMPORARY FIX) - Minor updates in `makeExampleBASiCS_Data` and `newBASiCS_Data` function to allow different spike-in types (as in `SingleCellExperiment`) version 1.3.2: (2018-05-21) - Version bump only version 1.3.3: (2018-05-21) - Missing line added to test_data_examples.R version 1.3.5: (2018-08-31) - Updated reference to latest paper version 1.3.6: (2018-09-04) - Updated CITATION file version 1.3.7: (2018-09-05) - Commit to activate Travis/codecov version 1.3.8: (2018-09-05) - Updated README.md (Travis/codecov badges) + updated citation to Cell Systems version 1.3.9: (2018-09-27) - Changed raw data accessor to 'counts' instead of 'assay' - makeExampleBASiCS_Data now generates 30 cells instead of 20 - Updated unit tests to match these 30 cells version 1.3.10: (2018-09-28) - Prevent BASiCS_Chain from crashing when StoreAdapt=TRUE for the regression case - Prevent plot function errors - Updated documentation using roxygen2 version 1.3.11: (2018-09-30) - Alan added as contributor version 1.3.13: (2018-09-30) - Some Rd files generated again version 1.3.15: (2018-10-07) - Fixed bug when storing chains in the non-spikes case version 1.3.16: (2018-10-08) - Reduce checks for Data that was not generated with the newBASiCS_Data function version 1.3.17: (2018-10-11) - New unit tests for different argument settings for the BASiCS_MCMC function version 1.3.18: (2018-10-15) - `BASiCS_D_TestDE` has been deprecated (replaced by `BASiCS_TestDE`) - `SingleCellExperiment::` added to all calls of `isSpike` - Extra input check in `BASiCS_MCMC` to avoid issues due to multiple types of spike-ins being present in the data. version 1.3.19: (2018-10-18) - Replacement of `R::lgammafn` by `std::lgamma` in c++ code (great speed-up! with thanks to Shian Su (@Shians)) version 1.3.20: (2018-10-18) - Minor updates to README and vignette (e.g. to include BASiCS sticker) version 1.3.21: (2018-10-19) - Restores use of `R::lgammafn` due to errors in Bioconductor build report version 1.3.22: (2018-10-19) - Update of DESCRIPTION to require C++11 - Once again, replacement of `R::lgammafn` by `std::lgamma` in c++ code version 1.3.23: (2018-10-18) - Updated vignette to include simple SCE example without spike-in information version 1.3.24: (2018-10-21) - Version bump to trigger new build version 1.5.1: (2018-11-29) - `ImmunoOncology` added in biocViews as requested by BioC core team version 1.5.2: (2018-12-01) - Fixes use of `BatchInfo` when it cannot be parsed to numeric - Refactors the use of `...` argument (several functions) version 1.5.3: (2018-12-06) - Removes `...` argument from `BASiCS_VarianceDecomp` - Adds missing argument (`beside`) to documentation of `BASiCS_VarianceDecomp` version 1.5.4: (2019-02-12) - Replaces `HPDinterval` by `coda::HPDinterval` in `BASiCS_showFit` method - Same as above when calling `mcmc` - Changes `BASiCS_showFit` such that fit line is on top of the dots - Additional optional arguments (`markExcludedGenes`, `colour`, `GenesSel` and `colourGenesSel`) added to `BASiCS_showFit` for more flexibility version 1.5.5: (2019-03-14) - Updated vignette to include recommended settings earlier. version 1.5.6: (2019-03-20) - merge from RELEASE_3_8 branch - Bug fix when not providing BatchInfo as factor - Extra check if batch names contain spaces - Added line breaks after individual error messages - Bug fix that corrects for the number of factors in BatchInfo vector - Small bug fix in 'test_mcmc_arguments.R' version 1.5.7: (2019-03-22) - Added default value for 'BatchInfo' within 'BASiCS_MCMC' version 1.5.8: (2019-03-22) - Changes DESCRIPTION to have a single maintainer - Density plot added to plot Method for BASiCS_Chain objects - Output plots for plot Method for BASiCS_Chain objects version 1.5.9: (2019-03-24) - Alan O'Callaghan is now author - `diagPlot` and `diagHist` methods added to facilitate diagnosis of convergence and mixing for MCMC chains - Added explicit calls to `ggplot2` in plot methods. - `BASiCS_DetectHVG` and `BASiCS_DetectLVG` edited based on the "regression" approach (i.e. using epsilon to detect HVGs/LVGs). - In `BASiCS_DetectHVG` and `BASiCS_DetectLVG`, options for `OrderVariable` were simplified (only original order and based on probability) - Temporary changes in no-spikes version have been restored version 1.5.10 (2019-03-26) - Corrected ordering in TestDE outpout so that genenames always match - Small fixes for R CMD check - Ordering in `BASiCS_DetectHVG` and `BASiCS_DetectLVG` can only be done on GeneNames, GeneIndex and Prob version 1.5.11 (2019-03-26) - Edits in BASiCS_Package.R so that `roxygen2` creates `NAMESPACE` - `testthat` moved from Imports to Suggests version 1.5.12 (2019-03-26) - R (>= 3.6) is now a requirement version 1.5.13 (2019-03-27) - `BASiCS_D_TestDE` from deprecated to defunct (follows BioC guidelines) version 1.5.14 (2019-03-27) - Brackets removed from `setGeneric` call (as per Hadley Wickham recommendation) - Removes full import of `ggplot2` in `BASiCS_Package.R` - `BASiCS_Package.R` is now in alphabetical order version 1.5.15 (2019-03-27) - Extended message to suggest additional QC when MCMC sampler fails version 1.5.16 (2019-03-30) - `SingleCellExperiment` moved to depends (DESCRIPTION) - Explicit calls to external packages (e.g. `SingleCellExperiment::`) - Unit tests for `BASiCS_diagHist` and `BASiCS_diagPlot` - `BASiCS_diagHist`, `BASiCS_diagPlot` and `BASiCS_showFit` moved from methods to functions - Internal functions marked as 'Hidden' - `BASiCS_diagHist`, etc moved into separate .R files version 1.5.17 (2019-03-30) - Minor changes in dependencies to pass R CMD check - Minor typo fix in documentation of `newBASiCS_Data` - `@export` call added to all methods version 1.5.19 (2019-03-31) - `stats4` added to imports in order to import `plot` S4 generic. - `KernSmooth` removed from DESCRIPTION. - Maintainer field removed from DESCRIPTION (use Author@R only) - Updates to travis integration to include BiocCheck version 1.5.20 (2019-04-01) - Updated documentation for `BASiCS_MCMC`, `BASiCS_DetectHVG`, `BASiCS_DetectLVG` and `BASiCS_VarThresholdSearchHVG_LVG` version 1.5.21 (2019-04-01) - Uses built-in chain in examples for `BASiCS_diagHist` and `BASiCS_diagPlot` version 1.5.22 (2019-04-02) - `HiddenCheckValidCombination`, `HiddenGeneParams` and `HiddenGeneParams` moved from `Methods.R` to `HiddenUtils.R` - `sapply` replaced by `vapply` in `HiddenCheckValidCombination`, `HiddenThresholdSearchDetectHVL_LVG.R` and `HiddenThresholdSearchTestDE.R` - Use of `set.seed` removed from `makeExampleBASiCS_Data` - Unit tests updated accordingly - Adds missing running examples to Rds in order to pass R CMD BioCheck version 1.5.23 (2019-04-02) - Minor edit to the documentation of `makeExampleBASiCS_Data` (explain why a fixed seed is no longer used) version 1.5.24 (2019-04-05) - Minor edit to the documentation of `makeExampleBASiCS_Data` (fixed link to `set.seed` documentation as it led to WARNINGS in Bioc-devel) version 1.5.25 (2019-04-07) - Minor change in `muUpdateNoSpikes` (C++ function in `utils_MCMCNoSpikes.cpp`) to avoid WARNINGS in bioc-devel related to integers vs unsigned integers version 1.5.26 (2019-04-08) - Similar changes applied to `utils_MCMCcppReg.cpp` and `utils_MCMCcppRegNoSpikes.cpp`. version 1.5.27 (2019-04-08) - Edits `BASiCS_TestDE` to avoid low posterior probability thresholds derived from the EFDR calibration. This alters some of the related 'Hidden' functions. - In `BASiCS_TestDE`, default values for `EFDR_M`, `EFDR_D` and `EFDR_R` were changed to 0.05. - Unit tests updated accordingly version 1.5.28 (2019-04-09) - Edited version of `BASiCS_Sim` to allow no spikes and multiple batches - Documentation updated accordingly version 1.5.29 (2019-04-10) - Unit test associated to `BASiCS_Sim` updated to use a fixed seed and to check exact values being reproducible version 1.5.30 (2019-04-11) - `BASiCS_effectiveSize` has been created as a wrapper of `coda::effectiveSize`. This is to resolve issues when `Param = 'epsilon'` as it contains `NA` for very lowly expressed genes that are excluded from the mean/over-dispersion trend. version 1.5.31 (2019-04-15) - Updated vignette to include ERCC calculation version 1.5.32 (2019-04-17) - Minor change in `BASiCS_MCMC` to avoid storing adaptive variances in the `BASiCS_Chain` object when `StoreAdapt = TRUE`. - New unit test to ensure the correct output in `BASiCS_Chain@parameters` - Minor change in `Summary` method for `BASiCS_Chain` class to avoid calculating posterior summaries when `designMatrix` is part of the `parameters` slot - Added `return` to the docummentation of `BASiCS_effectiveSize`. version 1.5.33 (2019-04-19) - Updated ERCC formula in the vignette. version 1.5.34 (2019-04-20) - Fixes typo in vignette