Changes in version 1.6.0: NEW FEATURES o buildSsTypePwms() function supports the possibility to select begin and end point of splice sites sequences from which PWMs are built. It also supports the possibility to paste splice sites to build PWM. BUG FIXES o interest.sequential() and interest() corrections to their object output option. o annotateU12() modified to work correctly with the new changes in Biostrings package. o buildSsTypePwms() corrected and modified to better suit data for all species from SpliceRack and U12DB. Changes in version 1.4.0: NEW FEATURES o interest() and interest.sequential() functions now support "IntSpan" method, allows counting intron- spanning reads. o psi() function is added. It calculates Psi values. o annotateU12() fucntion supports DNAStringSet objects as its refGenome input. o Improved vignette document. SIGNIFICANT USER-VISIBLE CHANGES o eBayesInterest() removed. BUG FIXES o interest.sequential() and interest() corrections to their object output option. o annotateU12() modified to work correctly with the new changes in Biostrings package.