## ----style, results = 'asis'-------------------------------------------------- BiocStyle::markdown() knitr::opts_chunk$set(fig.wide = TRUE, fig.retina = 3, error=FALSE, eval=TRUE) ## ----rawDiagLogo, out.width="50%", fig.cap="The octopussy `rawDiag` package logo by Lilly van de Venn."---- knitr::include_graphics("octopussy.png") ## ----installRawrr, eval=FALSE------------------------------------------------- # if (!require("BiocManager", quietly = TRUE)) # install.packages("BiocManager") # # BiocManager::install("rawrr") ## ----checkInstallRequirements------------------------------------------------- rawDiag::checkRawrr rawDiag::checkRawrr() rawrr::installRawrrExe() ## ----listrawrrAssemblyPath---------------------------------------------------- rawrr::rawrrAssemblyPath() rawrr::rawrrAssemblyPath() |> list.files() ## ----fetchFromExperimentHub--------------------------------------------------- library(ExperimentHub) ExperimentHub::ExperimentHub() -> eh normalizePath(eh[["EH3222"]]) -> EH3222 normalizePath(eh[["EH4547"]]) -> EH4547 (rawfileEH3222 <- paste0(EH3222, ".raw")) if (!file.exists(rawfileEH3222)){ file.copy(EH3222, rawfileEH3222) } (rawfileEH4547 <- paste0(EH4547, ".raw")) if (!file.exists(rawfileEH4547)){ file.copy(EH4547, rawfileEH4547) } c(rawfileEH3222, rawfileEH4547) -> rawfile ## ----header------------------------------------------------------------------- (rawfile |> lapply(FUN = rawrr::readFileHeader) -> rawFileHeader) ## ----readEH4547OrbitrapTrailerTable------------------------------------------- rawfile |> lapply(FUN = rawDiag::readRaw) |> Reduce(f = rbind) -> x #BiocParallel::bplapply(FUN = rawDiag::readRaw) |> ## ----listFUN------------------------------------------------------------------ library(rawDiag) ls("package:rawDiag") |> grep(pattern = '^plot', value = TRUE) -> pm pm |> knitr::kable(col.names = "package:rawDiag plot functions") ## ----plotALL, fig.width = 10, fig.height = 5---------------------------------- pm |> lapply(FUN = function(plotFUN) { lapply(c('trellis'), function(method) { message("plotting", plotFUN, "using method", method, "...") do.call(plotFUN, list(x, method)) }) }) ## ----shinyApp----------------------------------------------------------------- rawfile |> dirname() |> rawDiag::buildRawDiagShinyApp() -> app ## ----runApp, eval=FALSE------------------------------------------------------- # shiny::runApp(app) ## ----FAQ1, eval=FALSE--------------------------------------------------------- # file.path(Sys.getenv("HOME"), "Downloads") |> # setwd() # # list.files() |> # grep(pattern = '*.raw$', value = TRUE) |> # lapply(FUN = rawDiag::readRaw) |> # Reduce(f = rbind) -> x ## ----sessioninfo, eval=TRUE--------------------------------------------------- sessionInfo()