SimFFPE

This package is for version 3.14 of Bioconductor; for the stable, up-to-date release version, see SimFFPE.

NGS Read Simulator for FFPE Tissue


Bioconductor version: 3.14

The NGS (Next-Generation Sequencing) reads from FFPE (Formalin-Fixed Paraffin-Embedded) samples contain numerous artifact chimeric reads (ACRS), which can lead to false positive structural variant calls. These ACRs are derived from the combination of two single-stranded DNA (ss-DNA) fragments with short reverse complementary regions (SRCRs). This package simulates these artifact chimeric reads as well as normal reads for FFPE samples on the whole genome / several chromosomes / large regions.

Author: Lanying Wei [aut, cre]

Maintainer: Lanying Wei <lanying.wei at uni-muenster.de>

Citation (from within R, enter citation("SimFFPE")):

Installation

To install this package, start R (version "4.1") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("SimFFPE")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("SimFFPE")
An introduction to SimFFPE PDF R Script
Reference Manual PDF
NEWS Text

Details

biocViews Alignment, DataImport, MultipleComparison, SequenceMatching, Sequencing, Software
Version 1.6.0
In Bioconductor since BioC 3.11 (R-4.0) (4 years)
License LGPL-3
Depends Biostrings
Imports dplyr, foreach, doParallel, truncnorm, GenomicRanges, IRanges, Rsamtools, parallel, graphics, stats, utils, methods
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package SimFFPE_1.6.0.tar.gz
Windows Binary SimFFPE_1.6.0.zip (32- & 64-bit)
macOS 10.13 (High Sierra) SimFFPE_1.6.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/SimFFPE
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/SimFFPE
Bioc Package Browser https://code.bioconductor.org/browse/SimFFPE/
Package Short Url https://bioconductor.org/packages/SimFFPE/
Package Downloads Report Download Stats
Old Source Packages for BioC 3.14 Source Archive