## ---- include = FALSE--------------------------------------------------------- knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) ## ----setup, eval=FALSE-------------------------------------------------------- # library(shiny) # library(Seurat) # library(ggrepel) # library(shinydashboard) # library(schex) # library(iSEE) ## ---- eval=FALSE-------------------------------------------------------------- # pbmc_small <- make_hexbin(pbmc_small, nbins = 10, dimension_reduction = "PCA") # df_label <- make_hexbin_label(pbmc_small, "RNA_snn_res.0.8") ## ---- eval=FALSE-------------------------------------------------------------- # app <- shinyApp( # server= function(input, output){ # # output$all_genes <- renderUI({ # selectInput(inputId = "gene", label = "Gene", # choices = rownames(pbmc_small)) # }) # # output$plot1 <- renderPlot({ # plot_hexbin_meta(pbmc_small, "RNA_snn_res.0.8", action="majority", # title="Clusters") + guides(fill=FALSE) + # ggrepel::geom_label_repel(data=df_label, # aes(x=x, y=y, label=label), colour="black", # label.size=NA, fill=NA) # # }) # # output$plot2 <- renderPlot({ # plot_hexbin_feature(pbmc_small, type=input$type, feature=input$gene, # action=input$action, title=input$gene) # }) # # # }, # ui= dashboardPage(skin = "purple", # dashboardHeader(), # dashboardSidebar( # uiOutput("all_genes"), # radioButtons("type", "Type of expression:", # c("Raw" = "counts", # "Normalized" = "data")), # radioButtons("action", "Summarize using:", # c("Proportion not 0" = "prop_0", # "Mean" = "mean", # "Median" = "median")) # # ), # dashboardBody( # fluidRow( # box(plotOutput("plot1", width = 450, height=400), width=6), # box(plotOutput("plot2", width = 500, height=400), width=6)) # ) # ) # ) ## ----convert, eval=FALSE------------------------------------------------------ # pbmc_small <- as.SingleCellExperiment(pbmc_small) # pbmc_small <- make_hexbin(pbmc_small, nbins=10, dimension_reduction = "PCA") ## ---- eval=FALSE-------------------------------------------------------------- # plot_hexbin_gene_new <- function(sce, rows=NULL, rownames=character(0), # columns=NULL, type="logcounts", action="prop_0"){ # # plot_hexbin_feature(sce, type=type, feature=rownames, action=action) # } ## ---- eval=FALSE-------------------------------------------------------------- # schex_plot_gene <- customDataPlotDefaults(pbmc_small, 1) # schex_plot_gene$Function <- "plot_hexbin_gene_new" # schex_plot_gene$Arguments <- "type counts\naction prop_0\nrownames ODC1" # schex_plot_gene$ColumnSource <- "NULL" # schex_plot_gene$RowSource <- "NULL" # schex_plot_gene$DataBoxOpen <- TRUE # # # app <- iSEE( # pbmc_small, # customDataArgs=schex_plot_gene, # initialPanels=DataFrame( # Name=c("Custom data plot 1"), # Width=c(12)), # customDataFun=list(plot_hexbin_gene_new=plot_hexbin_gene_new) # )